Optimization of protein extraction from <i>Chlorella Vulgaris</i> via novel sugaring‐out assisted liquid biphasic electric flotation system

Koyande, Apurav Krishna; Chew, Kit Wayne; Lim, Jun-Wei; Lee, Sze Ying; Show, Pau Loke

doi:10.1002/elsc.201900068

Cited by 27 publications

(5 citation statements)

References 31 publications

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“…The study was performed in a small-scale LBF system of 50 mL. The initial parameters were set to 25 mL of 250 g/L glucose, 25 mL of 100% acetonitrile, 1:1 bottom phase and top phase ratio, 5 min flotation time and 50% microalgae biomass concentration determined from the previous work [10,18,19].…”

Section: Optimization Of Operating Parametersmentioning

confidence: 99%

Lipid Recovery from Microalgae Biomass Using Sugaring-Out Extraction in Liquid Biphasic Flotation System

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Chew

et al. 2023

Fermentation

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The increase in global temperature calls for ambitious action to reduce the release of greenhouse gases into the atmosphere. The transportation sector contributes up to 25% of the total emissions released, mainly from the burning of vehicle fuel. Therefore, scientists from all around the world are focusing on finding a sustainable alternative to conventional vehicle fuel. Biofuel has attracted much attention, as it shows great potential for the replacement of traditional fossil fuels. However, the main bottlenecks of biofuel are the ongoing controversial conflict between food security with biofuel production. Therefore, this study focuses on a sustainable extraction of lipids from microalgae for the production of biofuel using a liquid biphasic flotation system coupled with sugaring-out method. This is the first study to combine the methods of liquid biphasic flotation system with the sugaring-out technique. It represents a holistic study of optimum and effective conditions needed to extract lipids from the system and to understand the reliability of sugar solution as the agent of cell disruption. At the 15-min flotation time, 150 g/L of fructose solution with a 1:2 mass separating agent-acetonitrile ratio successfully extracted up to 74% of lipid from Chlorella sorokiniana CY-1. Two types of fatty acid methyl esters were recovered from the study, with C5:0 being the main component extracted.

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Section: Optimization Of Operating Parametersmentioning

confidence: 99%

Lipid Recovery from Microalgae Biomass Using Sugaring-Out Extraction in Liquid Biphasic Flotation System

Aron

Chew

et al. 2023

Fermentation

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“…However, it is possible to integrate electric cell disruption and LBF in just one step (i.e., extraction followed by purification), the so-called liquid biphasic electric flotation (LBEF) [209]. Koyande et al [210] were able to increase separation efficiency (nearly 10%) and protein recovery (nearly 20%) by replacing LBF with LBEF in protein extraction from C. vulgaris.…”

Section: Aqueous Two-phase Systemsmentioning

confidence: 99%

Microalgae Biomolecules: Extraction, Separation and Purification Methods

et al. 2020

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Several microalgae species have been exploited due to their great biotechnological potential for the production of a range of biomolecules that can be applied in a large variety of industrial sectors. However, the major challenge of biotechnological processes is to make them economically viable, through the production of commercially valuable compounds. Most of these compounds are accumulated inside the cells, requiring efficient technologies for their extraction, recovery and purification. Recent improvements approaching physicochemical treatments (e.g., supercritical fluid extraction, ultrasound-assisted extraction, pulsed electric fields, among others) and processes without solvents are seeking to establish sustainable and scalable technologies to obtain target products from microalgae with high efficiency and purity. This article reviews the currently available approaches reported in literature, highlighting some examples covering recent granted patents for the microalgae’s components extraction, recovery and purification, at small and large scales, in accordance with the worldwide trend of transition to bio-based products.

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“…The proteins are solubilized in organic solvents containing surfactants. The proteins are transferred from the aqueous phase to the organic phase via electrostatic interactions between proteins and surfactants [92]. The parameters driving this process are pH, concentration and type of salts utilized and type of organic solvents [25].…”

Section: Protein Fractionmentioning

confidence: 99%

Bio-processing of algal bio-refinery: a review on current advances and future perspectives

et al. 2019

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Microalgae biomass contains various useful bio-active components. Microalgae derived biodiesel has been researched for almost two decades. However, sole biodiesel extraction from microalgae is time-consuming and is not economically feasible due to competitive fossil fuel prices. Microalgae also contains proteins and carbohydrates in abundance. Microalgae are likewise utilized to extract high-value products such as pigments, anti-oxidants and long-chain polyunsaturated fatty acids which are useful in cosmetic, pharmaceutical and nutraceutical industry. These compounds can be extracted simultaneously or sequentially after biodiesel extraction to reduce the total expenditure involved in the process. This approach of bio-refinery is necessary to promote microalgae in the commercial market. Researchers have been keen on utilizing the bio-refinery approach to exploit the valuable components encased by microalgae. Apart from all the beneficial components housed by microalgae, they also help in reducing the anthropogenic CO 2 levels of the atmosphere while utilizing saline or wastewater. These benefits enable microalgae as a potential source for bio-refinery approach. Although life-cycle analysis and economic assessment do not favor the use of microalgae biomass feedstock to produce biofuel and co-products with the existing techniques, this review still aims to highlight the beneficial components of microalgae and their importance to humans. In addition, this article also focuses on current and future aspects of improving the feasibility of bio-processing for microalgae bio-refinery.

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Optimization of protein extraction from Chlorella Vulgaris via novel sugaring‐out assisted liquid biphasic electric flotation system

Cited by 27 publications

References 31 publications

Lipid Recovery from Microalgae Biomass Using Sugaring-Out Extraction in Liquid Biphasic Flotation System

Lipid Recovery from Microalgae Biomass Using Sugaring-Out Extraction in Liquid Biphasic Flotation System

Microalgae Biomolecules: Extraction, Separation and Purification Methods

Bio-processing of algal bio-refinery: a review on current advances and future perspectives

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