“…In both SeNP-Ionic and SeNP-Covalent modifications, the amount of selenium incorporated into the PhG NPs was determined by hydrolyzing PhG NPs with 70% nitric acid and quantifying by an UV-vis colorimetric assay using standard curves generated from known concentrations of Na Sel as described previously. 31 The prepared SeNP (SeNP-Ionic or SeNP-Covalent) were further characterized for particle size using previously described methods. 29 Figure 1 Illustration of the synthesis of the two selenium formulations.…”
Purpose
Selenium is an essential trace element that supports animal health through the antioxidant defense system by protecting cells from oxidative-related damage. Using inorganic selenium species, such as sodium selenite (Na Sel), as a food supplement is cost-effective; however, its limitation as a nutritional supplement is its cytotoxicity. One strategy to mitigate this problem is by delivering inorganic selenium using a nanoparticle delivery system (SeNP).
Methods
Rainbow trout intestinal epithelial cells, bovine turbinate cells and bovine intestinal myofibroblasts were treated with soluble Na Sel or SeNPs. Two SeNP formulations were tested; SeNP-Ionic where inorganic selenium was ionically bound to cationic phytoglycogen (PhG) NPs, and SeNP-Covalent, where inorganic selenium was covalently bound to PhG NPs. Selenium-induced cytotoxicity along with selenium bioavailability were measured.
Results
SeNPs (SeNP-Ionic or SeNP-Covalent) substantially reduced cytotoxicity in all cell types examined compared to similar doses of soluble inorganic selenium. The SeNP formulations did not affect selenium bioavailability, as selenium-induced glutathione peroxidase (GPx) activity and GPx1 transcript levels were similarly elevated whether cells were treated with soluble Na Sel or SeNPs. This was the case for all three cell types tested.
Conclusion
Nanoparticle-assisted inorganic selenium delivery, which demonstrated equal bioavailability without causing deleterious cytotoxic side effects, has potential applications for safely supplementing animal diets with inorganic selenium at what are usually toxic doses.
“…In both SeNP-Ionic and SeNP-Covalent modifications, the amount of selenium incorporated into the PhG NPs was determined by hydrolyzing PhG NPs with 70% nitric acid and quantifying by an UV-vis colorimetric assay using standard curves generated from known concentrations of Na Sel as described previously. 31 The prepared SeNP (SeNP-Ionic or SeNP-Covalent) were further characterized for particle size using previously described methods. 29 Figure 1 Illustration of the synthesis of the two selenium formulations.…”
Purpose
Selenium is an essential trace element that supports animal health through the antioxidant defense system by protecting cells from oxidative-related damage. Using inorganic selenium species, such as sodium selenite (Na Sel), as a food supplement is cost-effective; however, its limitation as a nutritional supplement is its cytotoxicity. One strategy to mitigate this problem is by delivering inorganic selenium using a nanoparticle delivery system (SeNP).
Methods
Rainbow trout intestinal epithelial cells, bovine turbinate cells and bovine intestinal myofibroblasts were treated with soluble Na Sel or SeNPs. Two SeNP formulations were tested; SeNP-Ionic where inorganic selenium was ionically bound to cationic phytoglycogen (PhG) NPs, and SeNP-Covalent, where inorganic selenium was covalently bound to PhG NPs. Selenium-induced cytotoxicity along with selenium bioavailability were measured.
Results
SeNPs (SeNP-Ionic or SeNP-Covalent) substantially reduced cytotoxicity in all cell types examined compared to similar doses of soluble inorganic selenium. The SeNP formulations did not affect selenium bioavailability, as selenium-induced glutathione peroxidase (GPx) activity and GPx1 transcript levels were similarly elevated whether cells were treated with soluble Na Sel or SeNPs. This was the case for all three cell types tested.
Conclusion
Nanoparticle-assisted inorganic selenium delivery, which demonstrated equal bioavailability without causing deleterious cytotoxic side effects, has potential applications for safely supplementing animal diets with inorganic selenium at what are usually toxic doses.
“…[32][33][34][35][36][37][38][39][40][41][42] Extractive spectrophotometric determination of selenium (IV) has been reported using a sensitive reagent allied with direct extractionspectrophotometric determination at quantitative level using selective solvent. [43][44][45] The reported methods were sensitive with some merits but they are also associated with serious drawbacks viz: narrow beer's range, need of temperature controlled oxidation condition for catalytic and catalytic kinetic determination, laborious procedure, interferences from foreign ions, need of heating for complex formation, long time equilibration for quantitative extraction, less stability of the complex and rarely available reagent. Thus the detailed survey of literature explains that there is a wide range for the work on development of a sensitive methodology for the quantitative recovery of selenium (IV) with minimum drawbacks.…”
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