Extraction, separation and characterization of lipopeptides and phospholipids from corn steep water

Rodríguez‐López, Lorena; Rincón-Fontán, M.; Vecino, Xanel; Cruz, J.M.

doi:10.1016/j.seppur.2020.117076

Cited by 32 publications

(47 citation statements)

References 46 publications

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“…In a previous work (Rodríguez‐López et al, 2020) it was demonstrated that CSL contains sporulated Bacillus bacteria that are able to produce BS spontaneously in CSL during the steeping process. The BS contained in CSL was identified by Rodríguez‐López et al (2020) as a lipopeptide composed of fatty acids (C16‐Methyl palmitate and C18‐1n9Cis‐9‐Oleic acid methyl ester/ trans‐9‐elaidic acid methyl ester) and amino acids (glutamic acid/glutamine, aspartic acid/asparagine, glycine, alanine, arginine, proline, and leucine/isoleucine). Moreover, in this BS extract the presence of phospholipids compatible with phosphatidylethanolamine and phosphatidylcholine were also detected, these were separated and purified by Rodríguez‐López et al (2020).…”

Section: Resultsmentioning

confidence: 99%

“…The BS contained in CSL was identified by Rodríguez‐López et al (2020) as a lipopeptide composed of fatty acids (C16‐Methyl palmitate and C18‐1n9Cis‐9‐Oleic acid methyl ester/ trans‐9‐elaidic acid methyl ester) and amino acids (glutamic acid/glutamine, aspartic acid/asparagine, glycine, alanine, arginine, proline, and leucine/isoleucine). Moreover, in this BS extract the presence of phospholipids compatible with phosphatidylethanolamine and phosphatidylcholine were also detected, these were separated and purified by Rodríguez‐López et al (2020). In addition, the BS producer strain in CSL was isolated in a previous work, being characterized as an endospore‐forming Bacillus strain, similar to Bacillus mycoides (López‐Prieto et al, 2019a).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, in this work the BS extracts contained in CSL were obtained following the methodology used in previous works (Rodríguez‐López et al, 2020; Vecino et al, 2015) and identified applying different analytical methods described in the experimental section.…”

Section: Resultsmentioning

confidence: 99%

“…FTIR was used to identify the functional groups and chemical bonds of the four BS extracts obtained from different CSL lots (FEED and COPAM), already characterized in a previous work (Rodríguez‐López et al, 2020). For the analysis 1 mg of each BS extract was ground and pressed with 10 mg potassium bromide (7500 kg for 30 s) to obtain translucent pellets and recorded on a Nicolet 6700 FTIR system (Thermo Fisher Scientific, Massachusetts, USA), following the procedure established in previous works (López‐Prieto et al, 2019c; Vecino et al, 2015).…”

Section: Experiments Sectionmentioning

confidence: 99%

“…The presence of lipopeptides in the BS extracts were corroborated by spectrometry/collision‐induced dissociation (ESI), with a mass spectrometer Bruker FTMS APEXIII (Fremont, CA, USA) in positive mode, using the same protocol established in previous works (Rodríguez‐López et al, 2020).…”

Section: Experiments Sectionmentioning

confidence: 99%

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Towards more Ecofriendly Pesticides: Use of Biosurfactants Obtained from the Corn Milling Industry as Solubilizing Agent of Copper Oxychloride

López-Prieto

Moldes

Cruz

et al. 2020

J Surfact & Detergents

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Copper oxychloride (Cu-Oxy) is a widely used copper-based fungicide. A drawback of the product is its very low water solubility (around 0.2%) which limits its fungicide efficiency due to the low-penetrating capacity in the fungal mycelia. In this study, we have explored the use of biosurfactants (BS), surface-active compounds obtained by microbial production, to enhance the solubility of Cu-Oxy in agrochemical formulations. A BS extracted from corn steep liquor (CSL), a residue from the corn-milling industry, was evaluated as an additive to increase the solubility of Cu-Oxy in water. Different types of synthetic surfactants (Tween ® 80, sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB)) were included for comparison. Results showed that it was possible to dissolve around 90% of Cu-Oxy at concentration of 4 g L −1 in 20 min of contact time when the BS was added at the concentration of 20 g L −1. In contrast, the synthetic surfactants were only able to dissolve less than 0.5% of Cu-Oxy under the same conditions. In addition, a factorial design was carried out in order to evaluate the influence of BS concentration, Cu-Oxy concentration and contact time, on the solubilization of Cu-Oxy. Maximum percentage of solubilization (96.5%) was achieved at concentrations of 16.1 g L −1 of BS, 2 g L −1 of Cu-Oxy, and 20 min of contact time. These results allowed to conclude that BS extracted from CSL could be included in agrochemical formulations as solubilizing agents improving the stability of hydrophobic active principles and reducing the concentration needed of chemical pesticides, giving eco-friendlier formulations.

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Section: Resultsmentioning

confidence: 99%