Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage

Zhao, Ting; Zhou, Yi; Mao, Guanghua; Zou, Ye; Zhao, Jianwu; Bai, Shiqi; Yang, Liuqing

doi:10.1002/jsfa.5937

Cited by 35 publications

(23 citation statements)

References 29 publications

(29 reference statements)

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“…However, the latter possesses a more compact matrix and scarce chondrocytes, a description that does not correspond, to our knowledge, to any cartilage described so far in vertebrates. It is noticeable that most of the literature concerning sturgeon cartilage relies on studies aiming to evaluate the commercial or industrial potential of cartilage and its components (collagen, chondroitin sulfate…) (Mathews and Decker, ; Zhao et al, ; Liang et al, ), rather than on morphological, histological or ultrastructural studies. Further studies should, however, be undertaken in order to describe more accurately this typical cartilage type and particularly how matrix compactness and cell size change during ontogeny, from a “classic” cell‐rich hyaline cartilage in early stages to this original matrix‐rich cartilage.…”

Section: Discussionmentioning

confidence: 99%

Vertebral Development and Ossification in the Siberian Sturgeon (Acipenser Baerii), with New Insights on Bone Histology and Ultrastructure of Vertebral Elements and Scutes

Leprévost

Azaı̈s

Trichet

et al. 2016

The Anatomical Record

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In order to improve our knowledge on the vertebral development, structure and mineralization in Acipenseriformes, we undertook a study in a growth series of reared Siberian sturgeons (Acipenser baerii) using in toto clear and stain specimens, histological and ultrastructural observations, X-ray micro-tomography, and solid state NMR analyses. Scutes were also studied to compare the tissue structure and mineralization of endoskeletal and dermal skeletal elements. This study completes and clarifies previous investigations on vertebral development and architecture in sturgeons, and brings original data on the structure of (i) the perichondral bone that is progressively deposited around the vertebral elements during ontogeny, (ii) the typical cartilage composing these elements, and (iii) the scutes. In addition we provide data on the mineralization process, on the nature of the bone mineral phase, and on the growth dynamics of the vertebral elements. Anat Rec, 300:437-449, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Vertebral Development and Ossification in the Siberian Sturgeon (Acipenser Baerii), with New Insights on Bone Histology and Ultrastructure of Vertebral Elements and Scutes

Leprévost

Azaı̈s

Trichet

et al. 2016

The Anatomical Record

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“…Thus, lots of effort has been put to develop reliable and safe alternative routes to produce specific forms of CS (Kim, Yang, Park, & Boons, ). Recently, many studies have been conducted to extract CS from Prionace glauca (Vazquez, Blanco, Fraguas, Pastrana, & Perez‐Martin, ), Chinese sturgeon cartilage (Zhao et al, ), fish bone (He et al, ), and skate cartilage (Lignot, Lahogue, & Bourseau, ). In addition, several chemical methods for synthesis of chondroitin (Kobayashi, Fujikawa, & Ohmae, ; Lopin & Jacquinet, ; Sears & Wong, ), CS and its oligosaccharides (Eller, Collot, Yin, Hahm, & Seeberger, ; Lubineau & Bonnaffé, ) have been established.…”

Section: Introductionmentioning

confidence: 99%

A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

Zhou

Huang

et al. 2018

Biotech & Bioengineering

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Chondroitin sulfate has been widely used in both medical and clinical applications. Commercial chondroitin sulfate has been mainly acquired from animal tissue extraction. Here we report a new two-step biological strategy for producing chondroitin sulfate A and chondroitin sulfate C. First, the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source was systematically optimized and the titer of chondroitin was significantly enhanced to 7.15 g/L. Then, specific sulfation transformation systems were successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV), chondroitin 4-sulfotransferase (C4ST) and chondroitin 6-sulfotransferase (C6ST). Chondroitin sulfate A and C were enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The present biological strategy has great potential to be scaled up for biosynthesis of chondroitin sulfate A and C from cheap carbon sources.

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“…On a dry basis, the protein content of sturgeon cartilage in this study was 52.73%, which was different from Siberian sturgeon cartilage (49.11%) (39) and cartilage powder of Acipenser sinensis (58.72%) (40). The differences in protein content may be the results of the species and separation processing technology (41)(42)(43).…”

Section: Discussionmentioning

confidence: 53%

Anti-inflammatory and Antioxidant Activity of Peptides From Ethanol-Soluble Hydrolysates of Sturgeon (Acipenser schrenckii) Cartilage

Chu

et al. 2021

Front. Nutr.

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Research has shown that cartilage containing chondroitin sulfate and protein presents versatile bioactivities. Chondroitin sulfate in cartilage is beneficial to activate the immune system while the protein/peptide has not been fully understood. The current study investigated the antioxidant and anti-inflammatory properties of ethanol-soluble hydrolysates of sturgeon cartilage (ESCH) prepared through hot-pressure, enzymatic hydrolysis and ethanol extraction. UV spectrum, IR and agarose gel electrophoresis results suggested the successful exclusion of chondroitin sulfate from peptides. Nitric oxide (NO) floods in cells activated by inflammation. It was inhibited when administrated with ESCH. To further explain the observed anti-inflammatory activity, ESCH was separated with Sephadex G-15 into 3 components, among which F3 showed a higher NO inhibition rate and significantly reduced the production of the proinflammatory cytokine IL-6. In addition, the yield of IL-10 increased. Western blotting suggested that F3 downregulated the NO content and IL-6 level by suppressing Mitogen-activated protein kinases (MAPK) channels. Moreover, both ESCH and F3 showed DPPH and ABTS free radical scavenging abilities which was possibly related to the anti-inflammatory property. These results indicated that ESCH behaved anti-inflammatory and antioxidant activities. Cartilage may be a good source to produce anti-inflammatory peptides.

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Extraction, purification and characterisation of chondroitin sulfate in Chinese sturgeon cartilage

Abstract: The results of this study provide a basis for promoting the utilisation of Chinese sturgeon resources and are significant for the development and utilisation of CS from Chinese sturgeon cartilage in the food industry.

Cited by 35 publications

References 29 publications

Vertebral Development and Ossification in the Siberian Sturgeon (Acipenser Baerii), with New Insights on Bone Histology and Ultrastructure of Vertebral Elements and Scutes

Vertebral Development and Ossification in the Siberian Sturgeon (Acipenser Baerii), with New Insights on Bone Histology and Ultrastructure of Vertebral Elements and Scutes

A microbial–enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

Anti-inflammatory and Antioxidant Activity of Peptides From Ethanol-Soluble Hydrolysates of Sturgeon (Acipenser schrenckii) Cartilage

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