Extraction procedures for the liquid chromatographic determination of thiamin, riboflavin and vitamin B6 in foodstuffs

Simultaneous determination of water‐soluble vitamins in selected food matrices by liquid chromatography/electrospray ionization tandem mass spectrometry

“…Furthermore, they may be bound tightly but non-covalently to proteins and polysaccharides. From an analysis of the scientific literature, [7][8][9] it transpires that these vitamins are most often determined in the free form, involving hydrolysis of the phosphorylated forms and/or those bound to matrix. Consequently, their extraction from different foods is performed by acid hydrolysis (0.1 M HCl/H 2 SO 4 , at 1008C or in an autoclave at 1218C) followed by enzymatic digestion (takadiastase and b-amylase).…”

Effect of Shelf life on Germination Percentage and Some Nutritional Value of Germinated Native Black Rice

“…Briefly,thiamin was extracted according the method of Ndaw et al (2000) [12]. Before analysis, the samples were filtered through a 0.45 um Millex filter (Millipore).…”

Simultaneous Determination of Water-Soluble Vitamins (Vitamin B1, B2, B3, B6and C) in Dietary Supplements by High-Performance Liquid Chromatography

“…The extraction procedure of vitamins from foods was necessary for the use of successive acid or enzymatic hydrolysis steps by viñas et al (2003) and Ndaw et al (2000). But vitamins in dietary supplements are easily extracted without specific preparation such as enzymatic or acid hydrolysis because vitamins are added as an isolation type into dietary supplements.…”

“…Because the extraction of vitamin B1 from foodstuffs normally requires the use of acid or enzymatic hydrolysis steps (Ndaw et al 2000;Viñas et al 2003), better result might be obtained by using mobile phase adjusted pH 3.5 with acid. Regardless of vitamin types, capsule type dietary supplements had better extraction efficiency in mobile phase than other solvents.…”