Extraction, partial purification and characterization of alkaline protease from rainbow trout (Oncorhynchus Mykiss) viscera

Andevari, Ghasem Taghizadeh; Rezaei, Masoud; Tabarsa, Mehdi; Rustad, Turid

doi:10.1016/j.aquaculture.2018.10.052

Cited by 11 publications

(1 citation statement)

References 34 publications

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“…The partial purification of ginger rhizome crude extract was performed by using ammonium sulfate precipitation technique as mentioned by Andevari et al (2019). The (NH 4 ) 2 SO 4 saturations to a concentration of 30, 40, 50, 60, 70, and 80% (w/v) were carried out simultaneously.…”

Section: Crude Extract Partial Purificationmentioning

confidence: 99%

Utilization of Partially Purified Milk Clotting Protease from Ginger Rhizome in the Manufacturing of Fresh Cheese

Maskey,

Pandey,

Shrestha

et al. 2023

Himalayan J. Sci. Technol.

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This study was carried out to utilize the partially purified ginger rhizome protease in fresh cheesemaking. The crude ginger rhizome extract was partially purified by employing 30-80% (NH4)2SO4 saturations (w/v). The maximum milk clotting activity was achieved at 50% saturation, exhibiting the purification fold of 1.64 and activity recovery of 85.76%. The milk pH and temperature for optimum time of coagulation and milk clotting activity were optimized by using response surface methodology (RSM). A numerical optimization study revealed that the optimum milk pH and temperature for producing cheese were 6.5 and 50 C respectively. The cheese produced by ginger protease had significantly (p<0.05) higher moisture, ash and yield, but lower fat content than the rennet cheese. No significant difference (p>0.05) was found in the calcium and protein content of both cheeses. The prepared cheese using ginger protease had significantly (p<0.05) higher spreadability, but lower aftertaste than the rennet cheese. However, non-significant difference (p>0.05) was observed in the flavor and overall acceptance between the two cheeses. Hence, this study demonstrated that the ginger rhizome protease has the potential to be utilized as an effective milk coagulating enzyme in the manufacturing of fresh cheese.

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Section: Crude Extract Partial Purificationmentioning

confidence: 99%