Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp.

Song, Hong; He, Meilin; Gu, Chuankun; Wang, Dong; Liang, Yuqi; Yan, Junmei; Wang, Changhai

doi:10.3390/polym10030292

Cited by 64 publications

(34 citation statements)

References 57 publications

(103 reference statements)

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“…The hot water extraction (HWE) was performed according to a previously reported method with some modifications [31]. Briefly, the snow chrysanthemum powders (1.0 g) were firstly refluxed with 10 mL of 80% ( v / v ) ethanol at 80 °C for 2 h to remove most of the small molecules.…”

Section: Methodsmentioning

confidence: 99%

Extraction Optimization and Effects of Extraction Methods on the Chemical Structures and Antioxidant Activities of Polysaccharides from Snow Chrysanthemum (Coreopsis Tinctoria)

Guo

Yuan

et al. 2019

Polymers

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In order to explore snow chrysanthemum polysaccharides (SCPs) as functional food ingredients and natural antioxidants for industrial applications, both microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) were firstly optimized for the extraction of SCPs. Furthermore, the effects of conventional hot water extraction, UAE, and MAE on the chemical structures and antioxidant activities of SCPs were investigated. The maximum extraction yields of SCPs extracted by UAE (4.13 ± 0.24%) and MAE (4.26 ± 0.21%) were achieved at the optimized extraction parameters as follows: ultrasound amplitude (68%) and microwave power (500 W), ultrasound extraction time (21 min) and microwave extraction time (6.5 min), and ratio of liquid to raw material (42.0 mL/g for UAE and 59.0 mL/g for MAE). In addition, different extraction methods significantly affected the contents of uronic acids, the molecular weights, the molar ratio of constituent monosaccharides, and the degree of esterification of SCPs. SCPs exhibited remarkable DPPH (IC50 ≤ 1.702 mg/mL), ABTS (IC50 ≤ 1.121 mg/mL), and nitric oxide (IC50 ≤ 0.277 mg/mL) radical scavenging activities, as well as reducing power (≥ 80.17 ± 4.8 μg Trolox/mg), which suggested that SCPs might be one of the major contributors toward the antioxidant activities of snow chrysanthemum tea. The high antioxidant activities (DPPH, IC50 = 0.693 mg/mL; ABTS, IC50 = 0.299 mg/mL; nitric oxide, IC50 = 0.105 mg/mL; and reducing power, 127.79 ± 2.57 μg Trolox/mg) observed in SCP-M extracted by the MAE method might be partially attributed to its low molecular weight and high content of unmethylated galacturonic acids. Results suggested that the MAE method could be an efficient technique for the extraction of SCPs with high antioxidant activity, and SCPs could be further explored as natural antioxidants for industrial application.

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Section: Methodsmentioning

confidence: 99%

Extraction Optimization and Effects of Extraction Methods on the Chemical Structures and Antioxidant Activities of Polysaccharides from Snow Chrysanthemum (Coreopsis Tinctoria)

Guo

Yuan

et al. 2019

Polymers

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“…Microalgal polysaccharides also showed potent activity toward different free radical scavenging [126][127][128]. Sun et al [54] obtained two polysaccharides (P-0 and S-0) from algae Pavlova viridis and Sarcinochrysis marina Geitler, and found that the degraded polysaccharides (P-2 and S-3) showed stronger antioxidant activity than the undegraded polysaccharides (P-0 and S-0) ( Table 1).…”

Section: Microalgal Polysaccharidesmentioning

confidence: 99%

The Antioxidant Activity of Polysaccharides Derived from Marine Organisms: An Overview

et al. 2019

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Marine-derived antioxidant polysaccharides have aroused extensive attention because of their potential nutritional and therapeutic benefits. However, the comprehensive comparison of identified marine-derived antioxidant polysaccharides is still inaccessible, which would facilitate the discovery of more efficient antioxidants from marine organisms. Thus, this review summarizes the sources, chemical composition, structural characteristics, and antioxidant capacity of marine antioxidant polysaccharides, as well as their protective in vivo effects mediated by antioxidative stress reported in the last few years (2013–2019), and especially highlights the dominant role of marine algae as antioxidant polysaccharide source. In addition, the relationships between the chemical composition and structural characteristics of marine antioxidant polysaccharides with their antioxidant capacity were also discussed. The antioxidant activity was found to be determined by multiple factors, including molecular weight, monosaccharide composition, sulfate position and its degree.

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“…The reagent used to dissolve the alginate oligosaccharides was set as the blank, and Vc was used as the positive control. The percentage of scavenged ABTS radical was calculated with the following equation:where A is the absorbance of the blank at 734 nm, and B is the final absorbance of each sample at 734 nm.The scavenging activity of hydroxyl radical was measured in accordance with previously reported methods(Song et al, 2018) with slight modifications. In brief, FeSO 4 (0.1 ml, 9.0 mM), deionized water (0.6 ml), and ethanol salicylate (0.1 ml, 9.0 mM) were mixed with a polysaccharide solution (0.2 ml).…”

mentioning

confidence: 99%

Cloning, expression, and characterization of a new pH‐ and heat‐stable alginate lyase from Pseudoalteromonas carrageenovora ASY5

et al. 2019

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Alginate lyase is important in marine alginate degradation, and its enzymatic hydrolysates are excellent antioxidants. Here, we cloned a new alginate lyase, that is, Alg823, from the Gram‐negative marine bacterium Pseudoalteromonas carrageenovora ASY5. The optimal temperature and pH of Alg823 were 55°C and pH 8.0, respectively. After 30 min of incubation at 50°C, Alg823 could maintain over 75.0% of the maximum enzyme activity, suggesting its thermostability. The recombinant alginate lyase retained more than 80.0% of the maximum enzyme activity after it was treated at pH 6.0–10.0 and 4°C for 24 hr, indicating its excellent pH stability. Mg2+, Ca2+, Na+, and K+ could promote enzyme activity. Alginate oligosaccharides obtained by degradation with Alg823 displayed an excellent ability to scavenge ABTS, hydroxyl, and DPPH radicals. Alg823 showed potential for novel applications in alginate oligosaccharide production because of its pH tolerance and heat adaptation. Practical applications Alginate oligosaccharides produced by alginate degradation possess favorable properties, such as low molecular weight, high stability, and co‐dissolution with water. These oligosaccharides also have many biological activities. As such, they have been widely explored. Alginate oligosaccharides are prepared via three methods, namely, physical, chemical, and enzymatic methods. In chemical method, operational processes are difficult to thereby possibly damaging the unique structure of polysaccharides and causing environmental pollution. Although physical methods can overcome some of the shortcomings of chemical methods, their reaction is still difficult to control, and products are complicated. Conversely, enzymatic methods can has advantages of mild conditions, single product, and less pollution. Furthermore, oligosaccharides prepared by enzymatic methods are more biologically active than those prepared by other methods. Thus, finding novel alginate lyase with high activity and stability is important for research and commercial purposes.

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Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp.

Cited by 64 publications

References 57 publications

Extraction Optimization and Effects of Extraction Methods on the Chemical Structures and Antioxidant Activities of Polysaccharides from Snow Chrysanthemum (Coreopsis Tinctoria)

Extraction Optimization and Effects of Extraction Methods on the Chemical Structures and Antioxidant Activities of Polysaccharides from Snow Chrysanthemum (Coreopsis Tinctoria)

The Antioxidant Activity of Polysaccharides Derived from Marine Organisms: An Overview

Cloning, expression, and characterization of a new pH‐ and heat‐stable alginate lyase from Pseudoalteromonas carrageenovora ASY5

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