Extraction optimization of polyphenols, oxindole alkaloids and quinovic acid glycosides from cat's claw bark by Box–Behnken design

Kaiser, Samuel; Verza, Simone Gasparín; Moraes, Renata Cougo; Pittol, Vanessa; Peñaloza, Evelyn Maribel Condori; Pavei, Cabral; Ortega, George González

doi:10.1016/j.indcrop.2013.04.026

Cited by 38 publications

(14 citation statements)

References 20 publications

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“…The extraction from cat’s claw bark was performed with hydro-ethanolic solutions 50% (v/v) by 2 h dynamic maceration in magnetic stirring plate (300 rpm) (RO 15 Power, IKA, Germany) at room temperature (23 ± 1 °C) (Kaiser et al. 2013a ), followed by filtration and concentration under vacuum at 40 °C up to half of their original weight (BüchiR-114, Germany) aiming to eliminate the ethanol. The concentrated extract was stand overnight in cold (10 ± 1 °C).…”

Section: Methodsmentioning

confidence: 99%

In vitro synergism of a water insoluble fraction of Uncaria tomentosa combined with fluconazole and terbinafine against resistant non- Candida albicans isolates

Moraes

Carvalho

Lana

et al. 2016

Pharmaceutical Biology

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Context:Uncaria tomentosa D.C. (Rubiaceae) has several biological activities, including activity against resistant Candida strains. The synergistic interaction with terbinafine or fluconazole can be an important alternative to overcome this resistance.Objectives: The potential synergy between a water insoluble fraction (WIF) from Uncaria tomentosa bark and the antifungals terbinafine (TRB) and fluconazole (FLZ) against non-Candida albicans resistant strains was investigated.Materials and methods: TRB and FLZ, alone and combined with WIF, were tested by the checkerboard procedure using the micro-dilution technique against seven isolates of Candida glabrata and C. krusei. The molecular interactions occurring outside the cell wall were evaluated by scanning electron microscopy, Fourier transform infrared (FT-IR) and differential scanning calorimetry (DSC) analysis.Results: The checkerboard inhibitory assay demonstrated synergy for WIF:TRB and WIF:FLZ combinations, respectively. The best synergistic cell damage was demonstrated unequivocally for the associations of WIF and TRB (1.95:4.0 μg/mL) and WIF and FLZ (1.95:8.0 μg/mL). The comparison of the FT-IR spectra of the antifungal alone, and in combination with WIF, allows recognizing clear differences in 3000, 1600, 1400, and 700–800 cm−1 bands. Additionally, modifications on TRB and FLZ thermograms were clearly noticed after their combination with WIF.Conclusions: DSC and infrared analysis demonstrated intermolecular interactions between WIF and either TRB or FLZ. Hence, quite likely the synergistic effect is related to interaction events occurring outside the cell wall between antifungal and cat’s claw proanthocyanidins. A direct action on the cell wall is suggested, without connection with the ABC efflux pump mechanism.

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Section: Methodsmentioning

confidence: 99%

In vitro synergism of a water insoluble fraction of Uncaria tomentosa combined with fluconazole and terbinafine against resistant non- Candida albicans isolates

Moraes

Carvalho

Lana

et al. 2016

Pharmaceutical Biology

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“…Pharm Sci Asia 2017; 44 (3), [154][155][156][157][158][159][160][161] techniques are collected for designing experiments, building models, evaluating the effects of factors and searching optimum condition of factors for desirable responses 9 .It has been successfully reported that RSM using Box-Behnken design can be used to optimize the extraction conditions from plants to obtain active compounds 9,12 . No such study has been carried out on the extraction of antioxidants from P. barbata leaves.…”

Section: Original Articlementioning

confidence: 99%

Optimization of antioxidant extraction from Persicaria barbata leaves using response surface methodology (RSM)

Jaroennon¹,

Sithisarn²,

Rojsanga³

2017

Pharm Sci Asia

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Optimization of antioxidant capacity from Persicaria barbata leaf extract was conducted using response surface methodology (RSM). The conditions investigated were 90 -150 min extraction time (x 1 ), 60 -75%v/v ethanol concentration (x 2 ) and 30 -50 v/w liquid-to-solid ratio (x 3 ) while a fixed extraction temperature at 95 °C was chosen. The quality of fit to the second-order polynomial models was confirmed based on the coefficient of determination of 0.9581. Box-Behnken experimental design indicated that models can significantly (p< 0.05) express more than 80% ( > 0.80) of the response variation. The order of factors influencing the response value of antioxidant capacity was ethanol concentration, extraction time and liquid-to-solid ratio, respectively. The optimal extract condition for maximum antioxidant capacity was 120 min of extraction time, 60% (v/v) of ethanol concentration, 40: 1 mL/g of liquid-to-solid ratio and extraction temperature at 95 °C. The ascorbic acid equivalent antioxidant capacity (AEAC) value obtained from experiment was 93.825 ± 0.15 mg AEAC/ g of dried plant.

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“…The oxindole alkaloid content was determined using an HPLC-PDA method (Kaiser et al, 2013) employing mitraphylline (Sigma-Aldrich, USA) as external standard. Alkaloid content was calculated by the sum of individual alkaloid concentrations (ex: speciophylline, uncarine F, mitraphylline, isomitraphylline, pteropodine, isopteropodine).…”

Section: Content Assay Of Oxindole Alkaloids By Hplc-pdamentioning

confidence: 99%

Antifungal activity of Uncaria tomentosa (Willd.) D.C. against resistant non-albicans Candida isolates

Moraes

Lana

Kaiser

et al. 2015

Industrial Crops and Products

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Extraction optimization of polyphenols, oxindole alkaloids and quinovic acid glycosides from cat's claw bark by Box–Behnken design

Cited by 38 publications

References 20 publications

In vitro synergism of a water insoluble fraction of Uncaria tomentosa combined with fluconazole and terbinafine against resistant non- Candida albicans isolates

In vitro synergism of a water insoluble fraction of Uncaria tomentosa combined with fluconazole and terbinafine against resistant non- Candida albicans isolates

Optimization of antioxidant extraction from Persicaria barbata leaves using response surface methodology (RSM)

Antifungal activity of Uncaria tomentosa (Willd.) D.C. against resistant non-albicans Candida isolates

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