Extraction optimization and in vitro and in vivo anti-postprandial hyperglycemia effects of inhibitor from Phoenix dactylifera L. parthenocarpic fruit

Abed, Hanen El; Chakroun, Mouna; Fendri, Imen; Makni, Mohamed; Bouaziz, Mohamed; Drira, Noureddine; Mejdoub, Hafedh; Khemakhem, Bassem

doi:10.1016/j.biopha.2017.01.129

Cited by 32 publications

(33 citation statements)

References 39 publications

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“…αglycosidase blocks the intestine glucose in the small intestine and α-amylase block the hydrolysis of complex starches for oligosaccharides. This inhibition system reduces the rate of digestion of carbohydrates [9,20]. Regular intake of natural antioxidants such as glutathione and phenolic reduces the risk of several chronic diseases including cancer, diabetes and cardiovascular disease [21,22].…”

Section: Resultsmentioning

confidence: 99%

Effects of dates (Phoenix Dactylifera Linn) on Blood Glucose Levels in Type-2 Diabetes Mellitus Patients

Wijayanti¹,

Pamungkasari²,

Sugiarto³

2019

Proceedings of the Proceedings of the 1st Seminar and Workshop on Research Design, for Education, Social Science, Arts, and Hum

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Type-2 diabetes mellitus (DM) burdens health care costs when complications occurred. Dates (Phoenix dactylifera Linn) contain almost complete nutrients, fiber and rich in antioxidants, which can control blood glucose levels. This study aimed to determine the effect of dates on blood glucose levels. This was the experimental study with Randomized Control Trial Pretest and Posttest. There were 36 outpatients of type-2 DM, divided into two groups, control and treatment. 50 grams of Lulu dates were given to the treatment group a day, while the control group were not. In 14 days, all groups were continued to consume oral anti-diabetic drugs. Daily intake data were obtained from 24-hr recall questionnaires, nutritional status data obtained from anthropometric measurements while data of DM from blood glucose levels. Data analysis using the Paired t-test. Results showed that the difference in the decrease of blood glucose levels, in control group (8.67 mg/dl, p-value=0.067) were lower than the treatment group (22.94 mg/dl, p-value=0.04). There were no significant decrease of blood glucose levels between control and treatment groups (p-value=0.223). In this study, consumption of dates has no significant effect on blood glucose levels, but further study are needed to know how dates can improve blood glucose levels.

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Section: Resultsmentioning

confidence: 99%

Effects of dates (Phoenix Dactylifera Linn) on Blood Glucose Levels in Type-2 Diabetes Mellitus Patients

Wijayanti¹,

Pamungkasari²,

Sugiarto³

2019

Proceedings of the Proceedings of the 1st Seminar and Workshop on Research Design, for Education, Social Science, Arts, and Hum

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“…The effect of substrate concentration was maximal at 1.0% of starch solution in case of leaves and stems ( Figure 5 ). This concentration was also frequently used for amylase assay in previous researches [ 55 , 56 ]. However, the substrate concentration starting to increase from 0.5% was significantly followed by the enzyme activity increase with enhancement of 23.81 and 31.74%, respectively, for leaves and stems.…”

Section: Resultsmentioning

confidence: 99%

Optimization, Purification, and Starch Stain Wash Application of Two Newα-Amylases Extracted from Leaves and Stems ofPergularia tomentosa

Lahmar

Abed

Khemakhem

et al. 2017

BioMed Research International

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A continuous research is attempted to fulfil the highest industrial demands of natural amylases presenting special properties. New α-amylases extracted from stems and leaves of Pergularia tomentosa, which is widespread and growing spontaneously in Tunisia, were studied by the means of their activities optimization and purification. Some similarities were recorded for the two identified enzymes: (i) the highest amylase activity showed a promoted thermal stability at 50°C; (ii) the starch substrate at 1% enhanced the enzyme activity; (iii) the two α-amylases seem to be calcium-independent; (iv) Zn2+, Cu2+, and Ag2+ were considered as important inhibitors of the enzyme activity. Following the increased gradient of elution on Mono Q-Sepharose column, an increase in the specific activity of 11.82-fold and 10.92-fold was recorded, respectively, for leaves and stems with the presence of different peaks on the purification profiles. Pergularia amylases activities were stable and compatible with the tested commercial detergents. The combination of plant amylase and detergent allowed us to enhance the wash performance with an increase of 35.24 and 42.56%, respectively, for stems and leaves amylases. Characterized amylases were reported to have a promoted potential for their implication notably in detergent industry as well as biotechnological sector.

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“…The aqueous ethanolic extract from P. dactylifera parthenocarpic date was prepared using previously described protocol [ 8 ]. Briefly, the dried powdered of healthy palm parthenocarpic dates (15 g) was macerated in 100 mL 71% ethanol solution (pH = 8.5) for 24 h at 59°C under stirring condition.…”

Section: Methodsmentioning

confidence: 99%

“…Dates and their constituents act as potent antioxidant, anti-inflammatory, and antitumoral and provide a suitable alternative therapy in various diseases cure [ 2 ]. According to the Inter Professional Fruits Group (GIFruits), dates production in Tunisia reached 246.000 tons in 2015, 40% of which is disposed or recycled as animal feed [ 8 ]. These are mainly dates attacked by pests, fermented and parthenocarpic dates (Sish).…”

Section: Introductionmentioning

confidence: 99%

“…These are mainly dates attacked by pests, fermented and parthenocarpic dates (Sish). The previous study has shown that the aqueous ethanolic extract from P. dactylifera parthenocarpic dates contains several bioactive components such as p-coumaric acid hexose, rosmadial, quercetin, quercetin-3,7-di-O-glucoside, and ganodermic acid [ 8 ]. Therefore, the objective of this study was to evaluate the antioxidant, the anti-inflammatory, and the antiproliferative activities of the aqueous ethanolic extract from P. dactylifera parthenocarpic dates.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant, Anti-Inflammatory, and Antitumoral Effects of Aqueous Ethanolic Extract fromPhoenix dactyliferaL. Parthenocarpic Dates

Abed

Chakroun

Abdelkafi-Koubaa

et al. 2018

BioMed Research International

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The aim of this study was to evaluate the antioxidant, the anti-inflammatory, and the antitumoral activities of the aqueous ethanolic extract from Phoenix dactylifera L. parthenocarpic dates. The antioxidant activity was carried using DPPH radical scavenging activity. The result showed that parthenocarpic dates had strongly scavenging activity on DPPH reaching 94% with an IC50 value of 0.15 ± 0.011 mg/mL (p < 0.05). The anti-inflammatory potential was determined by the inhibitory effect of the aqueous ethanolic extract on phospholipase A2 activity as well as on carrageenan-induced paw oedema in mice. The in vitro study showed that the extract inhibited the phospholipase A2 activity with an IC50 value of 130 μg/mL and the in vivo study showed a significantly decrease in the paw oedema after 1 h compared to the control group. Finally, the antiproliferative activity of the aqueous ethanolic extract was assessed by MTT test against MCF-7 and MDA-MB-231 cancer cell lines. This extract was effective in inhibiting MDA-MB-231 and MCF-7 cancer cells growth with IC50 values of 8 and 18 mg/mL, respectively, after 72 h treatment. These results confirm the ethnopharmacological significance of Phoenix dactylifera L. parthenocarpic dates, which could add support for its pharmaceutical use.

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Extraction optimization and in vitro and in vivo anti-postprandial hyperglycemia effects of inhibitor from Phoenix dactylifera L. parthenocarpic fruit

Cited by 32 publications

References 39 publications

Effects of dates (Phoenix Dactylifera Linn) on Blood Glucose Levels in Type-2 Diabetes Mellitus Patients

Effects of dates (Phoenix Dactylifera Linn) on Blood Glucose Levels in Type-2 Diabetes Mellitus Patients

Optimization, Purification, and Starch Stain Wash Application of Two Newα-Amylases Extracted from Leaves and Stems ofPergularia tomentosa

Antioxidant, Anti-Inflammatory, and Antitumoral Effects of Aqueous Ethanolic Extract fromPhoenix dactyliferaL. Parthenocarpic Dates

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