Extraction of β‐Glucan from Oat Bran in Laboratory Scale

Abstract

Effects of various enzymes and extraction conditions on yield and molecular weight of β‐glucans extracted from two batches of commercial oat bran produced in Sweden are reported. Hot‐water extraction with a thermostable α‐amylase resulted in an extraction yield of ≈76% of the β‐glucans, while the high peak molecular weight was maintained (1.6 × 106). A subsequent protein hydrolysis significantly reduced the peak molecular weight of β‐glucans (by pancreatin to 908 × 103 and by papain to 56 × 103). These results suggest that the protein hydrolyzing enzymes may not be pure enough for purifying β‐glucans. The isolation scheme consisted of removal of lipids with ethanol extraction, enzymatic digestion of starch with α‐amylase, enzymatic digestion of protein using protease, centrifugation to remove insoluble material, removal of low molecular weight components using dialysis, precipitation of β‐glucans with ethanol, and air‐drying.

Extraction and chemical characterization of rye arabinoxylan and the effect of β-glucan on the mechanical and barrier properties of cast arabinoxylan films

“…The measured b-glucan content was 7.5% which is also in the range of values reported earlier (Immerstrand et al, 2009;Johansson et al, 2000).…”

“…The oat bran was obtained using a disc milling process similar to that applied to rye bran, which provided a bran material containing 47% starch (Table 1), comparable to that reported in previous publications (Butt, Tahir-Nadeem, Khan, Shabir, & Butt, 2008;Immerstrand et al, 2009;Westerlund, Andersson, & Åman, 1993). …”

Gastrointestinal Release of β‐Glucan and Pectin Using an In Vitro Method

“…Several authors (Beer et al 1997;Monro 1991Monro , 1993Monro , 2000 have questioned the use of these methods for different types of fibers due to their limited relevance to gut-soluble fibers, and a new method for the assay of integrated total dietary fiber is applying a protocol more consistent with the human digestive system (McCleary et al 2010). However, our attempts with the same method for oat bran resulted in a low yield of βglucan (Immerstrand et al 2009). However, our attempts with the same method for oat bran resulted in a low yield of βglucan (Immerstrand et al 2009).…”

Extraction of β‐Glucan from Oats for Soluble Dietary Fiber Quality Analysis

“…We have also detected β-glucan hydrolase activity in xylanase preparations (data not shown). Immerstrand et al (2009) noted β-glucan hydrolase activity in pepsin and pancreatin preparations. Ironically, our own tests with pepsin and pancreatin treatments indicated no β-glucan hydrolase activities in the preparations we used (data not shown).…”