Extraction of total alkaloids, peimine and peiminine from the flower of Fritillaria thunbergii Miq using supercritical carbon dioxide

Ruan, Xiuxiu; Yang, Li; Li, Zhaohui; Liu, Ben; Wang, Qiang

doi:10.1016/j.jcou.2017.01.024

Cited by 31 publications

(15 citation statements)

References 36 publications

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“…Since bulbs constitute the most critical parts of Fritillaria plants and are the primary source of isosteroidal alkaloids in Fritillaria species used in traditional Chinese medicine (TCM), the production of bulbs in F. cirrhosa by tissue culture technology is highly desirable. The objective of the present study was to investigate the effects of LED lights on the growth and development of embryogenic callus and the analysis of alkaloid contents in cultures; however, the development of bulblets in somatic embryos is an important observation in the study because in natural conditions, one bulb typically develops into a single seedling and it takes about 5–6 years to grow into an appropriate size [ 46 ]. It has also been reported that isosteroidal alkaloids in F. cirrhosa bulbs are greatly influenced by environmental conditions, plant age, and harvest times [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

Chen

Lee

et al. 2020

Plants

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Investigations were carried out to study the effects of light-emitting diode (LED) lights on growth and development of isosteroidal alkaloids in embryogenic calli of Fritillaria cirrhosa D. Don, an important traditional Chinese medicine herb. Calli were cultured in glass bottles, each containing 100 mL of Murashige and Skoog’s basal medium supplemented with 2% sucrose and 0.4% gellan gum powder, a gelling agent. These bottles were incubated in a specially designed plant growth chamber equipped with eight different LED lights consisting of single or combinations of four different light spectra emitting blue (450 nm), green (525 nm), red (660 nm), and far-red (730 nm) light. After three months of incubation, morphological changes in embryogenic calli were recorded, and LC-MS/MS analysis of cultures was carried out for peimisine, sipeimine, peiminine, and peimine. The highest number of somatic embryos and the maximum fresh weight was recorded in calli incubated under red (9R), infrared (9IR), and a combination of red+blue+infrared (3R3B3IR), respectively, in decreasing order. The highest contents of peimisine, peiminine, and peimine were recorded under red (9R) and infrared (9IR) lights, respectively. Eight LED lights had significant effects on the morphogenesis of embryogenic calli of F. cirrhosa D. Don and contents of isosteroidal alkaloids.

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Section: Resultsmentioning

confidence: 99%

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

Chen

Lee

et al. 2020

Plants

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“…SFE technique is highly selective for alkaloids, the solubility of alkaloids in Sc CO 2 increases on increasing the solvent density up to 813-850 kg/m 3 , and after that it may decrease. The use of basified alcohol mixtures as co-solvents enhances alkaloid yield from plant materials [110,113].…”

Section: Extraction Of Alkaloidsmentioning

confidence: 99%

A critical analysis of extraction techniques used for botanicals: Trends, priorities, industrial uses and optimization strategies

Belwal

Ezzat

Rastrelli

et al. 2018

TrAC Trends in Analytical Chemistry

349

182

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Plant extracts have been long used by the traditional healers for providing health benefits and are nowadays suitable ingredient for the production of formulated health products and nutraceuticals. Traditional methods of extraction such as maceration, percolation, digestion, and preparation of decoctions and infusions are now been replaced by advanced extraction methods for increased extraction efficiency and selectivity of bioactive compounds to meet up the increasing market demand. Advanced techniques use different ways for extraction such as microwaves, ultrasound waves, supercritical fluids, enzymes, pressurized liquids, electric field, etc. These innovative extraction techniques, afford final extracts selectively rich in compounds of interest without formation of artifacts, and are often simple, fast, environmentally friendly and fully automated compared to existing extraction method. The present review is focused on the recent trends on the extraction of different bioactive chemical constituents depending on the nature of sample matrices and their chemical classes including anthocyanins, flavonoids, polyphenols, alkaloids, oils, etc. In addition, we review the strategies for designing extraction, selection of most suitable extraction methods, and trends of extraction methods for botanicals. Recent progress on the research based on these advanced methods of extractions and their industrial importance are also discussed in detail.

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“…powder (particle size in the range of 0.210-0.149 mm) equal to 17.44 mg/g, using scCO 2 and 10% methanol as solvent at 333 K/8 MPa. Likewise, other alkaloids have been extracted by SFE-co such as the extraction of isoquinolidine alkaloids from C. majus L. terrestrial parts using isopropanol/diethylamine mixture as a cosolvent with an extraction yield equal to 15.80 mg/g (Gañán et al, 2016); peimine and pimine (isosteroidal alkaloids with quinolizidine nucleous) extraction using 90.3% ethanol as a cosolvent with an extraction yield of 2.9 mg/g (Ruan et al, 2017); matrine, oxysophocarpine and oxymatrine (quinolizidine alkaloids) extraction from Sophora flavescens Ait using 75% ethanol as a cosolvent with an extraction yield of 57.61 mg/g (a modifier ammonia-ethanol was added before SFE-co) (Ling, Zhang, Cui, & Zhang, 2007); nuciferine (isoquinoline alkaloid) extraction from Nelumbo nucifera Gaertn using 10% (vol/vol) diethylamine and 1% (vol/vol) water in methanol as cosolvent with an extraction yield of 0.33 mg/g (Xiao et al, 2010); and pyrrolidine alkaloids extraction from Piper amalago L. using 5% methanol as a cosolvent with an extraction yield of 3.8 mg/g (Carrara et al, 2017).…”

Section: Comparison Between Different Extraction Processmentioning

confidence: 99%

Supercritical fluid extraction with cosolvent of alkaloids from Lupinus mutabilis Sweet and comparison with conventional method

Rosas‐Quina

Mejía‐Nova

2021

J Food Process Engineering

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The purpose of this study was to evaluate the use of supercritical fluid extraction with cosolvent (SFE-co) as an alternative process in the extraction of lupin alkaloids. A sample of crushed Lupinus mutabilis Sweet seeds, with a high content of alkaloids, was treated using aqueous extraction (AQE) and SFE-co, where the effect of the operating conditions and the solvent in the SFE-co were evaluated applying a factorial design. Results showed that both methods reduced the content of total alkaloids in the lupin seed, ensuring its safe consumption. SFE-co got an extraction yield (39.19 ± 0.14 mg/g) comparable with AQE (39.22 ± 0.16 mg/g) at a temperature of 323 K, a pressure of 27 MPa, using as a solvent supercritical carbon dioxide (scCO 2) and dilute ethanol solution, but limited to a fraction of the treated sample. SFE-co, unlike AQE, was able to keep the nutritional value of the lupin seeds, reducing the time process from 235 hr (AQE) to 2.5 hr, assuring the non-use of an excessive amount of water and hazardous solvents. Moreover, the fractions formed in the extraction vessel by SFE-co allowed the understanding of the extraction mechanism of different components from a complex matrix related to their chemical polarity. Practical Applications This study shows that it is possible to remove lupin alkaloids with green technology as supercritical fluid extraction with cosolvents, reaching extraction yields comparable with the conventional method (aqueous extraction), keeping the nutritional value of the sample, saving time and resources. However, working with a larger quantity of material than the usual used in a laboratory showed an inhomogeneous extraction of alkaloids from the sample, limiting the optimal extraction yield to one of the fractions formed. This reveals a future problem in the use of this technology at an industrial level, where the characteristics of the extracts are not only important but also the material treated since, in the lupin industry, the debittered seed and the released alkaloids are useful in the food and pharmaceutical industries, respectively. 1 | INTRODUCTION Lupins are legumes whose cultivation has grown around the world, being the Lupinus albus, Lupinus luteus, Lupinus angustifoilius, and Lupinus mutabilis, the major species cultivated (Carvajal-Larenas, Linnemann, Nout, Koziol, & van Boekel, 2016). Among these species, L. mutabilis Sweet has aroused special interest in the research area due to its high nutritional value, registering a higher value of protein

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Extraction of total alkaloids, peimine and peiminine from the flower of Fritillaria thunbergii Miq using supercritical carbon dioxide

Cited by 31 publications

References 36 publications

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

A critical analysis of extraction techniques used for botanicals: Trends, priorities, industrial uses and optimization strategies

Supercritical fluid extraction with cosolvent of alkaloids from Lupinus mutabilis Sweet and comparison with conventional method

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