Extraction of testosterone and epitestosterone in human urine using aqueous two-phase systems of ionic liquid and salt

He, Chiyang; Li, Shehong; Liu, Huwei; Li, Kèan; Liu, Feng

doi:10.1016/j.chroma.2005.05.065

Cited by 267 publications

(206 citation statements)

References 33 publications

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“…While He et al [20] indicated that temperature had not significant influence on the distribution behavior of steroids, Pei et al [23], on the other hand, reported that temperature greatly influences the extraction efficiency of proteins. As a result it can be established that the partition coefficients dependence on temperature will largely depend on the solute and ternary system under study.…”

Section: Effect Of Temperature In Vanillin Partitioningmentioning

confidence: 99%

“…The effect of temperature on the partition coefficients of vanillin was studied with four ILs: [C 4 [20,23,[35][36], using IL-based ATPS, the temperature was shown to be either a negligible or a significant factor in the extraction of various types of biomolecules. While He et al [20] indicated that temperature had not significant influence on the distribution behavior of steroids, Pei et al [23], on the other hand, reported that temperature greatly influences the extraction efficiency of proteins.…”

Section: Effect Of Temperature In Vanillin Partitioningmentioning

confidence: 99%

“…It was already shown that ATPS formed by K 3 PO 4 and ILs are extremely advantageous for the partition of several biomolecules yielding larger partition coefficients than those conventionally obtained with polymer-inorganic salts or polymer-polymer ATPS [6,10,13]. The extractive potential of biomolecules using IL-based ATPS was previously studied for distinct compounds, such as testosterone and epitestosterone, alkaloids, antibiotics, bovine serum albumin, penicillin G, ltryptophan and food coulorants [6,10,13,[20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

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Extraction of vanillin using ionic-liquid-based aqueous two-phase systems

Cláudio

Freire

et al. 2010

Separation and Purification Technology

183

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a b s t r a c tTo explore proper extractive solvents and to design an optimized separation process it is highly imperative to understand the molecular-based phenomena governing the solutes partitioning. Moreover, the development of new techniques for the biomolecules separation and purification, while maintaining their functional characteristics unchanged, is still ongoing. Therefore, in this work, the partition coefficients of vanillin, a compound with well-known organoleptic properties, were determined using improved ionicliquid-based aqueous two-phase systems (ATPS). Three main parameters were evaluated through the vanillin partitioning process: the ionic liquid (IL) cation and anion structure, the temperature of equilibrium and the available concentration of vanillin in the global system. In all systems and conditions tested, vanillin preferentially migrates for the IL-rich phase. In addition, the three studied parameters largely influence the vanillin partitioning. In an attempt to elucidate the thermodynamics of the partitioning process, the standard molar thermodynamic functions of transfer of vanillin were also determined based on the temperature dependence data. These data indicate that the partition of vanillin results from an interplay between enthalpic and entropic contributions where both the IL anion and more complex cations play an essential role. Moreover, viscosities and densities of both aqueous phases were experimentally measured at the mass fraction compositions for which the partition coefficients were determined. The results gathered in this work indicate that IL-based ATPS can be further employed in the extraction and purification of vanillin from different matrices, as confirmed by the large partition coefficients obtained and improved low viscosity systems.

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Section: Effect Of Temperature In Vanillin Partitioningmentioning

confidence: 99%

Section: Effect Of Temperature In Vanillin Partitioningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extraction of vanillin using ionic-liquid-based aqueous two-phase systems

Cláudio

Freire

et al. 2010

Separation and Purification Technology

183

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“…Some research groups have also studied the aggregation and micelles formation of ionic liquids in aqueous solution [32]. As RTILs can dissolve many kinds of organic, organometallic and inorganic compounds [33,34], their applications in analytical chemistry have also received attentions recently [35][36][37][38][39]. He et al determined the testosterone and epitestosterone in human urine using cloud extraction method based on aqueous two-phase systems consisting of ionic liquid 1-butyl-3-methylimidazolium chloride, coupled with HPLC/spectrophotometry detection, the detection limit of 1 ng/mL was obtained [38].…”

Section: Introductionmentioning

confidence: 99%

“…As RTILs can dissolve many kinds of organic, organometallic and inorganic compounds [33,34], their applications in analytical chemistry have also received attentions recently [35][36][37][38][39]. He et al determined the testosterone and epitestosterone in human urine using cloud extraction method based on aqueous two-phase systems consisting of ionic liquid 1-butyl-3-methylimidazolium chloride, coupled with HPLC/spectrophotometry detection, the detection limit of 1 ng/mL was obtained [38]. Generally, cloud extraction method was a simple, rapid and effective sample pretreatment technique; however, some limitations still existed, such as the RTILs-rich phase was dense and it was not easy to be injected into HPLC for analysis; high concentration of ionic liquid in sampling solution may produce large interferential peaks.…”

Section: Introductionmentioning

confidence: 99%

Analysis of phthalates via HPLC-UV in environmental water samples after concentration by solid-phase extraction using ionic liquid mixed hemimicelles

Cai

Shi

et al. 2008

Talanta

155

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Ionic liquid mixed hemimicelles-based solid-phase extraction for the preconcentration of five phthalates in environmental water sample was investigated in this paper. A comparative study on the use of room temperature ionic liquids (RTILs) 1-hexyl-3-methylimidazolium bromide ([C 6 mim]Br) and 1-dodecyl-3-methylimidazolium bromide ([C 12 mim]Br)-coated silica as sorbents was presented. Owing to having bigger adsorption amounts for analytes [C 12 mim], Br-coated silica was selected as SPE material and the five analytes di-ethyl-phthalate (DEP), di-n-propyl-phthalate (DnPP), di-n-butyl-phthalate (DnBP), di-cyclohexyl-phthalate (DcHP) and di-(2-ethylhexyl)-phthalate (DEHP) can be quantitatively extracted under optimal conditions. The analytes retained on the cartridge were desorbed completely with 3 mL methanol (pH 2). Predominant factors influencing the extraction efficiency, such as RTILs concentration, pH value, ionic strength and breakthrough volume were discussed. The proposed method had been applied to determining the five phthalates in four environmental water samples and concentration factor of 600 was achieved easily. Detection limits obtained ranged between 0.12 and 0.17 g/L. The accuracy of this method was evaluated by recovery measurement on spiked samples, and good recovery results (85-107%) with relative standard deviation (R.S.D.) of below 6% were achieved.

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Analytical Applications of Ionic Liquids

Acree

Grubbs

2012

Encyclopedia of Analytical Chemistry

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Analytical methods involving ionic liquids (ILs) have significantly increased in recent years, in part because of the unique solubilization and physical properties on this class of organic compounds. This article provides an overview of the different applications of ionic liquids in analytical chemistry. The presentation is divided into a section describing the properties of liquids that are of interest to analytical chemists, followed by nine sections pertaining to simple biphasic liquid‐liquid extractions, liquid‐phase microextraction methods, ionic liquid‐based solid‐phase microextractions (SPME), thin layer chromatographic (TLC) and high‐performance liquid chromatographic (HPLC) methods, electromigration methods, gas‐liquid chromatographic (GLC) methods, supported ionic liquid membrane separations, mass spectrometric methods, electrochemical methods, and spectroscopic methods. Representative samples of each application are given, along with specific references to the ionic liquid used and type of sample analyzed.

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Extraction of testosterone and epitestosterone in human urine using aqueous two-phase systems of ionic liquid and salt

Cited by 267 publications

References 33 publications

Extraction of vanillin using ionic-liquid-based aqueous two-phase systems

Extraction of vanillin using ionic-liquid-based aqueous two-phase systems

Analysis of phthalates via HPLC-UV in environmental water samples after concentration by solid-phase extraction using ionic liquid mixed hemimicelles

Analytical Applications of Ionic Liquids

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