Extraction of peroxidase from waste Brassica oleracea used for the treatment of aqueous phenol in synthetic waste water

Deva, Ashutosh Nath; Arun, C.; Arthanareeswaran, G.; Sivashanmugam, P.

doi:10.1016/j.jece.2014.04.014

Cited by 20 publications

(7 citation statements)

References 22 publications

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“…This result was an agreement with some studies dealing with phenol removal by peroxidise [54,55]. The above results show that the adequate molar ratio of hydrogen peroxide dose to the initial phenol concentration ([H 2 O 2 ]/[Phenol]) is 2:1 in aqueous phase and 4:1 in silicone oil (47V20); from this, BRP-CLEAs require a higher dose of hydrogen peroxide in organic medium compared to that needed in aqueous phase.…”

Section: Effect Of the Hydrogen Peroxide Concentrationsupporting

confidence: 91%

A combination of absorption and enzymatic biodegradation: phenol elimination from aqueous and organic phase

Tandjaoui

Abouseoud

Couvert

et al. 2017

Environmental Technology

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Peroxidase from Brassica rapa was immobilized as cross-linked enzyme aggregates (CLEAs) and used to treat air containing phenol as a model molecule of volatile organic compounds (VOCs). Prior to an enzymatic treatment, phenol was absorbed into an aqueous or organic phase (silicone oil) to reach concentrations ranging from 20 to 160 mg/L. The process was carried out by introducing a desired weighing of BRP-CLEAs into preparations and reaction was started by injecting HO solution to the medium. Optimization of the reaction conditions in the organic solvent revealed an optimal contact time of 60 min, 60 mg/L of phenol concentration and 3 mM HO, leading to a maximum removal yield of 70% for 3.4 UI/mL of BRP-CLEAs. These results were compared to those obtained in an aqueous medium that showed 90% of degradation yield after 40 min in the following conditions, 90 mg/L of initial phenol amount, 2 mM of HO and 2.5 UI/mL of BRP-CLEAs. Parameters of the Michaelis-Menten model, Km and Vmax, were also determined for the reaction in both phases. Phenol removal by BRP-CLEAs in silicone oil succeeded with 70% of conversion yield. It is promising regarding the transposition of such enzymatic process to hydrophobic VOCs.

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Section: Effect Of the Hydrogen Peroxide Concentrationsupporting

confidence: 91%

A combination of absorption and enzymatic biodegradation: phenol elimination from aqueous and organic phase

Tandjaoui

Abouseoud

Couvert

et al. 2017

Environmental Technology

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“…First, in the range from 0.5 to 6 mM, a significant amount of 2-CP was degraded by BRP-CLEAs and maximal conversion (31%) was obtained at an initial rate of 0.247 mg L -1 min -1 . A dramatic decrease of the rate and the yield of conversion were then observed beyond 6 mM of H2O2.This phenomenon was also encountered during phenol degradation by peroxidases, as a consequence of the partial inactivation of peroxidases due to the excess of hydrogen peroxide (Bódalo et al, 2008;Deva et al, 2014).…”

Section: Effect Of the Hydrogen Peroxide Concentrationmentioning

confidence: 81%

A new combined green method for 2-Chlorophenol removal using cross-linked Brassica rapa peroxidase in silicone oil

et al. 2016

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This study proposes a new technique to treat waste air containing 2-Chlorophenol (2-CP), namely an integrated process coupling absorption of the compound in an organic liquid phase and its enzymatic degradation. Silicone oil (47V20) was used as an organic absorbent to allow the volatile organic compound (VOC) transfer from the gas phase to the liquid phase followed by its degradation by means of Cross-linked Brassica rapa peroxidase (BRP) contained in the organic phase. An evaluation of silicone oil (47V20) absorption capacity towards 2-CP was first accomplished by determining its partition coefficient (H) in this solvent. The air-oil partition coefficient of 2-CP was found equal to 0.136 Pa m(3) mol(-1), which is five times lower than the air-water value (0.619 Pam(3) mol(-1)). The absorbed 2-CP was then subject to enzymatic degradation by cross-linked BRP aggregates (BRP-CLEAs). The degradation step was affected by four parameters (contact time; 2-CP, hydrogen peroxide and enzyme concentrations), which were optimized in order to obtain the highest conversion yield. A maximal conversion yield of 69% and a rate of 1.58 mg L(-1) min(-1)were obtained for 100 min duration time when 2-CP and hydrogen peroxide concentrations were respectively 80 mg L(-1) and 6 mM in the presence of 2.66 UI mL(-1) BRP-CLEAs. The reusability of BRP-CLEAs in silicone oil was assessed, showing promising results since 59% of their initial efficiency remained after three batches.

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“…O setor industrial concentra as atividades que mais contribuem para a contaminação ambiental. O grande consumo de água leva à produção de vários rejeitos de produtos líquidos contendo substâncias tóxicas recalcitrantes, as quais acabam por ser despejadas em corpos hídricos de forma inadequada (SALLES, P. T. F. PELEGRINI, N. N. B. PELEGRINI, 2006).…”

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Gestão De Resíduos Sólidos Urbanos E Rotas De Tratamento: Um Panorama Do Brasil E Do Mundo

Araújo¹,

Lange²,

Torres³

2021

Coleção Desafios Das Engenharias: Engenharia Sanitária

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Extraction of peroxidase from waste Brassica oleracea used for the treatment of aqueous phenol in synthetic waste water

Cited by 20 publications

References 22 publications

A combination of absorption and enzymatic biodegradation: phenol elimination from aqueous and organic phase

A combination of absorption and enzymatic biodegradation: phenol elimination from aqueous and organic phase

A new combined green method for 2-Chlorophenol removal using cross-linked Brassica rapa peroxidase in silicone oil

Gestão De Resíduos Sólidos Urbanos E Rotas De Tratamento: Um Panorama Do Brasil E Do Mundo

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