Extraction of p-hydroxyacetophenone and catechin from Norway spruce needles. Comparison of different extraction solvents

Abstract

The phenolic compounds p-hydroxyacetophenone and catechin have been extracted from Norway spruce needles with pure methanol, 80 and 50% (v/v) aqueous methanol, pure acetonitrile, 80% (v/v) aqueous acetonitrile, and pure water. Extraction efficiency of the individual solvents was compared. Although 80% aqueous methanol is the solvent most frequently used for extraction of soluble phenolic compounds from needles, it was found that pure methanol is a more suitable extraction solvent. Surprisingly, a two-step procedure based on the extraction of crushed needles with water then re-extraction with methanol proved a good alternative to direct extraction with methanol. Extraction of uncrushed spruce needles might indicate that relatively more p-hydroxyacetophenone than catechin was located in the surface layer of the needle.

Decomposition of spruce litter needles of different quality by Setulipes androsaceus and Thysanophora penicillioides

“…Litter needles represent the main source of p-HAP in the soil ecosystem (Vrchotová et al 2004), although p-HAP may also be rinsed from canopy needles by rainwater, and thus be introduced by percolating through the soil (Hongve et al 2000;Vosmanská et al 2005;Muscolo and Sidari 2006). In the winter, p-HAP may also rise through pores in the soil and enrich the snow cover (Gallet and Pellisier 1997).…”

“…Catechin and p-hydroxyacetophenone (p-HAP) were extracted following the methods of Vosmanská et al (2005). We used a two-step extraction (water, methanol) in an ultrasonic bath.…”

Evaluation of a liquid chromatography method for compound‐specific δ13C analysis of plant carbohydrates in alkaline media

“…The cation 'H' (H + form, removal of amino acids) and anion 'A' (HCO 3 – form, removal of organic acids) exchange cartridges allowed the neutral fractions to pass through. The function of the third 'P' cartridge was to remove phenolic compounds present in the plant water extracts . If these compounds were not removed, they would irreversibly bind to the CarboPac resin.…”