Extraction of IgE-Binding Components of <i>Helicobacter pylori</i> by Immunoblotting Analysis in Chronic Urticaria Patients

Abstract

Background: An association between Helicobacter pylori and chronic urticaria has been suspected previously. An IgE-mediated pathway might be a possible link between H. pylori infection and chronic urticaria, and therefore we wanted to prepare an optimal H. pylori antigen to detect H. pylori-specific IgE antibodies in chronic urticaria patients. Methods:H. pylori antigen extracts were prepared in different ways to find the optimal antigen extract to be used in the assays. Immunoblotting was used to detect IgE-binding bands. The results were applied in an H. pylori RAST assay for specific H. pylori IgE antibodies in patient sera. Results: In immunoblotting, the largest number of IgE-stained bands were visualized in the washing fluids and sonicated extracts, while strong heating and denaturing treatments destroyed the epitopes for IgE binding, suggesting that they belonged to the flagellar structures of H. pylori. However, in H. pylori-specific RAST analysis, specific IgE was found only in 1 of 25 H. pylori-infected patients. Conclusions: Our findings suggest that although IgE-binding epitopes were found in H. pylori, H. pylori-specific IgE antibodies are not common in chronic urticaria, and the clinical significance of the IgE response is unclear.

Systematic review and meta‐analysis: Effect ofHelicobacter pylorieradication on chronic spontaneous urticaria

“…The direct effects of HP, including immunoglobulins, HP virulence genes, autoimmune reactions, have been considered in CSU development. Immunoglobulin (Ig) G, IgA, and IgE antibodies against HP antigens were detected in some studies . The release of proinflammatory cytokines, granulocyte attraction, as well as B‐ and T‐cell–mediated responses, is elevated in HP‐positive patients, which may all contribute to the development of urticarial lesions .…”

“…Immunoglobulin (Ig) G, IgA, and IgE antibodies against HP antigens were detected in some studies. [34][35][36] The release of proinflam- disease. [40][41][42][43] Moreover, enhanced gastric permeability may also play a role; CSU is associated with a pseudo-allergy to food and increased permeability of the gastric mucosa, since damage caused by HP may give rise to inappropriate immunorecognition of food allergens.…”

Effect of Helicobacter pylori eradication in the treatment of Japanese patients with chronic idiopathic urticaria

“…Although several studies have been performed to identify immunogloblin-binding antigenic structures of H. pylori, 13,14 little is known about the antigenic structures that are responsible for CIU. Another possible mechanism is the increase of gastric 15,16 Recognition of food allergens may occur more easily in gastric mucosa infected with H. pylori.…”

Alzheimer′s Disease: APP, Gamma Secretase, APOE, CLU, CR1, PICALM, ABCA7, BIN1, CD2AP, CD33, EPHA1, and MS4A2, and Their Relationships with Herpes Simplex, C. Pneumoniae, Other Suspect Pathogens, and the Immune System

“…Mice immunised with inactivated herpes simplex develop IgE-specific antibodies to the virus [109]. High levels of IgE are also observed in man following recurrent herpes simplex infection [110] and human IgE antibodies are also known to interact with herpes family viruses including HSV-1 and 2 and the Epstein-Barr and cytomegalovirus [111] and also to C. pneumoniae, H. pylori, and B. burgdorferi [ [112][113][114][115]. IgE-related allergic responses are also involved in C. neoformans infection [116].…”