Extraction of Amana edulis Induces Liver Cancer Apoptosis

Abstract

HCC is one of the fastest-rising causes of cancer-related death. Novel therapeutic approaches for treatment are warranted. The goal of this study is to find effective components from Chinese herbal medicines, which is an important alternative source of anticancer medicine. To this end, six different herbs were selected from various traditional literatures. Soxhlet extractor was used to distill the strong polar and weak polar components of each herb. The inhibitive effect of each component was determined using liver cancer cells BEL7404. From total of 12 extractions, it was found that the combined crude lysate of Amana edulis from water and ethanol system had the best efficacy. At the concentration of 0.1 mg/mL, this component has the highest inhibition rate up to 70%. To investigate the underlying molecular reasons, we observed that the component can significantly induce the liver cancer cells apoptosis and retard the cell reproduction at G2/M stage. Verification experiments showed that this component also has apparent inhibitive effects on other liver cancer cells, such as HepG2 and Huh7. On the other hand, it has less effectiveness on another cell line HepaRG, which retains many characteristics of primary human hepatocytes. The results suggested that there might be highly efficient antihepatoma ingredient in the water and ethanol extraction of Amana edulis. The pure substances remain to be isolated and further research on their targets is required.

Promoting liver cancer cell apoptosis effect of Tribulus terrestris L. via reducing sphingosine level was confirmed by network pharmacology with metabolomics

“…Adjuvant therapy, including chemotherapy, radiation and targeted therapy, are used for advanced PLC patients. In addition, accumulated studies showed that traditional Chinese medicine (TCM) is an alternative treatment for PLC because of its effective therapeutic outcome and fewer side effects compared to chemo-radiotherapy [ 5 , 6 ]. For example, Bei-Jia-Jian-Wan and Xiao-Chai-Hu-Tang are two of the most frequently used Chinese herbal medicine for PLC.…”

Cytotoxic, apoptotic, and genetic evaluations of Nigella sativa essential oil nanoemulsion against human hepatocellular carcinoma cell lines

“…Different therapeutic strategies can be used for treatment of liver cancer including hormonal, biochemical, chemotherapeutic and molecular targeted therapies (Shaaban et al 2014). In addition, early and recent studies showed the promising participation of phytochemicals and herbal medicine in liver cancer therapy strategies (Abdel-Hamid et al 2018;Fan et al 2018;Li et al 2014;Li and Martin 2011). Formulation of phytochemicals in the form of nanoparticles enhances its therapeutic activity due to different factors and mechanisms which are fully discussed (Manivannan et al 2021;Beg et al 2020).…”

Tuliposides H–J and Bioactive Components from the Bulb of Amana edulis

“…Tulipa edulis (Miq.) Baker belongs to the Liliaceae family and is a folk medicinal plant used to treat cancer diseases [ 1 , 2 ]. However, there have been few phytochemical and biological studies of this species reported to date.…”

“…However, there have been few phytochemical and biological studies of this species reported to date. For example, 95% and 50% EtOH extracts could induce apoptosis in human gastric (SGC-7901) [ 1 ] and hepatoma (BEL7404, HepG2, and Huh7) [ 2 ] carcinoma cells, respectively. The polysaccharides prepared from A. edulis have anti-oxidant properties, including DPPH, OH − , and ABTS + scavenging activities [ 3 , 4 , 5 ].…”