Cytotoxic, apoptotic, and genetic evaluations of Nigella sativa essential oil nanoemulsion against human hepatocellular carcinoma cell lines

Abdrabou, Ahmed; Edris, Amr E.

doi:10.1186/s12645-021-00101-y

Cited by 17 publications

(9 citation statements)

References 51 publications

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“…The nucleic acid sequences of the forward (F) and reverse (R) primers of Bax and Bcl‐2 genes compared with GAPDH, as a housekeeping gene, were shown as designed in recent Abd‐Rabou's published paper. [33]…”

Section: Methodsmentioning

confidence: 99%

“…The nucleic acid sequences of the forward (F) and reverse (R) primers of Bax and Bcl-2 genes compared with GAPDH, as a housekeeping gene, were shown as designed in recent Abd-Rabou's published paper. [33] Real-time PCR mixture consisted of 10 μL of SYBR PCR Master Mix, 1 μL of each primer (400 nM), 1 μL cDNA, and 7 μL Rnase-free water in a total volume of 20 μL. Amplification conditions and cycle counts were a temperature of 95 C for 15 min for the initial activation, followed by 40 cycles of denaturation at 95 C for 30 s, annealing at 55 C for 30 s, and elongation at 72 C for 30 s. Melting curves were performed after real-time PCR to demonstrate the specific amplification of each gene versus GAPDH as an internal control to normalize the level of target gene expression.…”

Section: Real-time Pcr Reactionsmentioning

confidence: 99%

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Spectral studies, thermal investigations, and anticancer activity of some divalent metal complexes derived from 2‐(4‐bromophenylamino)acetohydrazide ligand

Emara

El‐Sayed

Khalaf‐Allah

et al. 2022

Applied Organom Chemis

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A series of biologically active metal complexes was yielded from the reactions of 2‐(4‐bromophenylamino)acetohydrazide ligand (HL) with Co(II), Ni(II), Cu(II), Cd(II) chlorides, CuBr2, Cu(CH3COO)2, and Cu(ClO4)2 salts. The structure and stereochemistry of the afforded complexes were elucidated by means of elemental analyses, molar conductance, magnetic susceptibility, thermal analyses (TG/DTG), and spectral techniques (mass, IR, UV‐Vis, and 1H NMR). Elemental analyses along with molar conductance data demonstrated that all metal complexes are nonelectrolyte and were formed with 1L:1M, 1L:2M, and 2L:1M stoichiometry. IR spectral data provided an evidence for the neutral bidentate behavior of the hydrazide ligand toward metal ions via carbonyl oxygen and amino nitrogen atoms (ON donor). Magnetic susceptibility along with electronic absorption spectral results authenticated that all complexes have an octahedral geometry except, CuCl2 complex (3), which possessed a square planar one. The hydrazide ligand and some of its synthesized metal complexes were screened in vitro for their antitumor and apoptotic activity. The results showed that CuBr2 complex (4) has promising anticancer activities against breast MCF7, liver HepG2, and colorectal HCT 116 cancerous cells through induction of proapoptotic gene (Bax) and inhibit the antiapoptotic gene (Bcl‐2), thus increase the Bax/Bcl‐2 ratio. Moreover, these newly synthesized compounds were most potent and can be used as a therapeutic chemoprevention against these cancerous cells compared with doxorubicin (DOX), the currently available anticancer drug against solid tumors.

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Section: Methodsmentioning

confidence: 99%

Section: Real-time Pcr Reactionsmentioning

confidence: 99%

Spectral studies, thermal investigations, and anticancer activity of some divalent metal complexes derived from 2‐(4‐bromophenylamino)acetohydrazide ligand

Emara

El‐Sayed

Khalaf‐Allah

et al. 2022

Applied Organom Chemis

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“…Abd-Rabou noted that nanoemulsions of Nigella sativa-EO inhibited the proliferation of hepatocellular carcinoma cells more effectively than pure EO after a 24-hour incubation. Speci cally, these nanoemulsions demonstrated inhibition rates of ~ 29% and ~ 15% against HepG2 and Huh-7 cells, respectively 57 . Such antiproliferative effects are likely linked to the presence of terpinen-4-ol 58 and cinnamaldehyde 59 , key chemical components found in TEO and CEO.…”

Section: In Vitro Biological Characterisation Of the Lblfunctionalise...mentioning

confidence: 90%

Synergistic nanocoating: Layer-by-Layer functionalised PCL membranes enhanced with manuka honey and essential oils for advanced wound healing

Gallo,

Girón-Hernández,

Honey

et al. 2024

Preprint

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Chronic wounds represent a significant global health concern, statistically impacting 1–2% of the population in developed countries throughout their lifetimes. These wounds cause considerable discomfort for patients and necessitate substantial expenditures of time and resources for treatment. Among the emerging therapeutic approaches, medicated dressings incorporating bioactive molecules, including natural compounds, are particularly promising. Hence, the objective of this study was to develop novel antimicrobial dressings for wound treatment. Specifically, polycaprolactone membranes were manufactured using the electrospinning technique and subsequently coated with natural polyelectrolytes (chitosan as a polycation and a mixture of manuka honey with essential oils nanoemulsions as a polyanion) employing the Layer-by-Layer assembly technique. Physico-chemical and morphological characterisation was conducted through QCM-D, FTIR-ATR, XPS, and SEM analyses. The results from SEM and QCM-D demonstrated successful layer deposition and coating formation. Furthermore, FTIR-ATR and XPS analyses distinguished among different coating compositions. The coated membranes were tested in the presence of fibroblast cells, demonstrating biocompatibility and expression of genes coding for VEGF, COL1, and TGF-β1, which are associated with the healing process (assessed through RT-qPCR analysis). Finally, the membranes exhibited excellent antibacterial activity against both Staphylococcus aureus and Pseudomonas aeruginosa, with higher bacterial strain inhibition observed when cinnamon essential oil nanoemulsion was incorporated. Taken together, these results demonstrate the potential application of nanocoated membranes for biomedical applications, such as wound healing.

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“…It has crucial signaling functions in physiological and pathological processes such as brain signaling, immunological responses, and blood pressure regulation [79]. Excessive nitric oxide production has been linked to various harmful illnesses, including trauma, dermatitis, and cancer [80]. The nitric oxide scavenging activity of the synthesized AgNPs is given in Figure 6.…”

Section: Nitric Oxide Scavenging Activitymentioning

confidence: 99%

Investigation of Anti-hepatocarcinogenic Effects of Senna auriculata Silver Nanoparticle and Evaluation of Their Antioxidant Potential

Poornasundari,

Arivoli,

Sankarasivaraman

2023

cast

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Plant-based biogenic nanoparticle synthesis has appeared as a feasible alternative to conventional approaches to chemical synthesis. As a result, several environmentally benign methods for the quick production of silver nanoparticles have been published in recent years. The methods employ aqueous extracts of plant components like leaves, bark, and roots. In the present study, silver nanoparticles were synthesized from an aqueous leaf extract of S. auriculata. UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive x-ray analysis (EDAX) were employed to validate the synthesized nanoparticles. The UV analysis showed a peak range between 390-420 nm. FTIR showed the functional groups present in the silver nanoparticles (AgNPs). Moreover, the synthesized AgNPs were tested for their antimicrobial activity against both gram-negative and gram-positive bacterial strains. The antioxidant properties were studied with DPPH, hydrogen peroxide, and nitric oxide scavenging activity assays, and a cytotoxic assay was conducted against the Huh-7 cell line by a MTT study. The results showed that the synthesized silver nanoparticles possessed strong antibacterial, antioxidant, and cytotoxic activities against the Huh-7 cell line, indicating that the silver nanoparticles might be used in the pharmaceutical industry and for novel biological applications.

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Cytotoxic, apoptotic, and genetic evaluations of Nigella sativa essential oil nanoemulsion against human hepatocellular carcinoma cell lines

Cited by 17 publications

References 51 publications

Spectral studies, thermal investigations, and anticancer activity of some divalent metal complexes derived from 2‐(4‐bromophenylamino)acetohydrazide ligand

Spectral studies, thermal investigations, and anticancer activity of some divalent metal complexes derived from 2‐(4‐bromophenylamino)acetohydrazide ligand

Synergistic nanocoating: Layer-by-Layer functionalised PCL membranes enhanced with manuka honey and essential oils for advanced wound healing

Investigation of Anti-hepatocarcinogenic Effects of Senna auriculata Silver Nanoparticle and Evaluation of Their Antioxidant Potential

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