Extraction of fixed, stained protein bands form gels for micro amino acid analysis using o-phthaldialdehyde

Disaccharide Analysis and Molecular Mass Determination to Microgram Level of Single Sulfated Glycosaminoglycan Species in Mixtures Following Agarose-Gel Electrophoresis

“…If a given macromolecule in the gel is to be subjected to further analysis, suitable methods must be found for removing that component from the gel matrix. Currently, this analytical approach is generally used to separate and recover proteins for further analysis, such as amino acid evaluation, from unfixed or fixed, unstained or stained bands from polyacrylamide gel (32). D'Arcy et al reported a separation of CS oligosaccharides from a preparative polyacrylamide gel on a milligram scale (33), whereas separation of GAGs by monodimensional cellulose acetate electrophoretic system and recovery of single bands for quantitative (34) and qualitative (35) analysis was developed.…”

A Low Picomole Fluorometric Detection System for Amino Acid Analysis

“…Water was distilled before use twice from a quartz flask after being passed through a column packed with charcoal and an ion exchange resin. 4.6 x 125 mm). An additional reciprocal pump (JASCO, SP-024-2) with two plunger units, each of which worked separately, was used to introduce the NaCIO and OPA reagents into the eluate through dumper column (44,,-, 88,um glass beads, 2.0 x 250mm).…”

Amino acid sequence of the N‐terminal domain of calf thymus histone H2A.Z

“…Hydrolysates were fractionated on an Aminco Aminalyzer high-performance liquid chromatography system as in [17] except that the column was run at 49°C without ammonia traps. Individual amino acids were quantitated by comparing integrated peak areas with those of standards.…”