“…Water was distilled before use twice from a quartz flask after being passed through a column packed with charcoal and an ion exchange resin. 4.6 x 125 mm). An additional reciprocal pump (JASCO, SP-024-2) with two plunger units, each of which worked separately, was used to introduce the NaCIO and OPA reagents into the eluate through dumper column (44,,-, 88,um glass beads, 2.0 x 250mm).…”