Extraction of collagen-II with pepsin and ultrasound treatment from chicken sternal cartilage; physicochemical and functional properties

Akram, Ayesha Noreen; Zhang, Chunhui

doi:10.1016/j.ultsonch.2020.105053

Cited by 58 publications

(46 citation statements)

References 53 publications

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“…6 , the EAI of oxhide gelatin samples increased after 0–400-W ultrasonication ( P < 0.05) and peaked with 300-W ultrasonication—indicating that 300-W ultrasonication improved the migration ability of oxhide gelatin from water to the oil–water interface. The results were consistent with those of Akram and Zhang [48] , who found that the EAI increased after ultrasonication.…”

Section: Resultssupporting

confidence: 92%

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Ultrasonication promotes extraction of antioxidant peptides from oxhide gelatin by modifying collagen molecule structure

Gao

Wang

et al. 2021

Ultrasonics Sonochemistry

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Section: Resultssupporting

confidence: 92%

“…In collagen, thermal stability differences are correlated with structure diversification [48] . The changes in DSC thermograms in our control and treated group are presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrasonication promotes extraction of antioxidant peptides from oxhide gelatin by modifying collagen molecule structure

Gao

Wang

et al. 2021

Ultrasonics Sonochemistry

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“…In the control samples of gizzards, at the initial time, the matrix surface is more uniform and visually denser (Figure 6-B) and the collagen fibers have a multi-layered aggregated structure (Figure 6(a2)-C). A similar structure of collagen was observed by Akram and Zhang [31] when studying the microstructure of collagen extracted from chicken sternal cartilage. The destruction of structural components under the influence of ultrasound has been noted by several authors, which confirms a similar effect of exposure to the fibrillar proteins of a complex of proteolytic enzymes of bacteria [31][32][33].…”

Section: Scanning Electron Microscopy (Sem)supporting

confidence: 78%

Optimization of Microbial Hydrolysis Parameters of Poultry By-Products Using Probiotic Microorganisms to Obtain Protein Hydrolysates

2021

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In connection with the active growth of poultry processing, the issue of rational use of poultry by-products is urgent. The paper proposes hydrolysis of the gizzards of broiler chickens and hens of the parent stock with bifidobacteria liquid concentrate (BLC) and Propionix liquid concentrated starter culture (Propionix LCSC). The effect of enzymatic treatment on changes in the structural components of the gizzards was studied using scanning electron microscopy (SEM) and determination of the dispersed composition. As a result of the research, the multiple regression equations and the response surfaces were obtained, which describe the optimal parameters of the gizzard´s hydrolysis process. The temperature and the time of hydrolysis are factors that have a significant effect on the degree of hydrolysis. The results of the structural and microscopic analysis confirm the high hydrolysability of the by-products by changing its structure and increasing the number of smaller protein particles. It was found experimentally that the gizzards of hens are more susceptible to the action of probiotic microorganisms’ enzymes compared to the gizzards of broiler chickens.

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“…The sample was ≈100-fold concentrated by ultrafiltration on a Microcon Centrifugal filter unit with a 10 kDa molecular cut-off (MRCPRT010, Millipore, Burlington, MA, USA) to obtain the final collagen at 10 mg/mL. Collagen from GSCM and Type I collagen from the cattle dermis were isolated using a protocol described in [82], while Type II collagen was isolated from the tracheal cartilage by a protocol described in [83] omitting the use of pepsin. An amount of 10 µg of the proteins were diluted with an SDS-loading buffer supplemented with 100 mM DTT (20710, SERVA, Heidelberg, Germany) and heated at 95 • C for 5 min.…”

Section: Collagen Molecular Weight Estimation (Sds-page)mentioning

confidence: 99%

A Collagen Basketweave from the Giant Squid Mantle as a Robust Scaffold for Tissue Engineering

et al. 2021

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The growing applications of tissue engineering technologies warrant the search and development of biocompatible materials with an appropriate strength and elastic moduli. Here, we have extensively studied a collagenous membrane (GSCM) separated from the mantle of the Giant squid Dosidicus Gigas in order to test its potential applicability in regenerative medicine. To establish the composition and structure of the studied material, we analyzed the GSCM by a variety of techniques, including amino acid analysis, SDS-PAGE, and FTIR. It has been shown that collagen is a main component of the GSCM. The morphology study by different microscopic techniques from nano- to microscale revealed a peculiar packing of collagen fibers forming laminae oriented at 60–90 degrees in respect to each other, which, in turn, formed layers with the thickness of several microns (a basketweave motif). The macro- and micromechanical studies showed high values of the Young’s modulus and tensile strength. No significant cytotoxicity of the studied material was found by the cytotoxicity assay. Thus, the GSCM consists of a reinforced collagen network, has high mechanical characteristics, and is non-toxic, which makes it a good candidate for the creation of a scaffold material for tissue engineering.

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Extraction of collagen-II with pepsin and ultrasound treatment from chicken sternal cartilage; physicochemical and functional properties

Cited by 58 publications

References 53 publications

Ultrasonication promotes extraction of antioxidant peptides from oxhide gelatin by modifying collagen molecule structure

Ultrasonication promotes extraction of antioxidant peptides from oxhide gelatin by modifying collagen molecule structure

Optimization of Microbial Hydrolysis Parameters of Poultry By-Products Using Probiotic Microorganisms to Obtain Protein Hydrolysates

A Collagen Basketweave from the Giant Squid Mantle as a Robust Scaffold for Tissue Engineering

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