Extraction of 3′,4′-anhydrovinblastine from Catharanthus roseus

Goodbody, Anne E.; Watson, C.D.; Chapple, Clint; Vukovic, John; Misawa, Masanaru

doi:10.1016/0031-9422(88)80430-8

Cited by 25 publications

(11 citation statements)

References 14 publications

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“…However, since the most recently published isolation procedures6 for VBL and VCR emphasize acidic extraction conditions, it appeared that the ‘anthocyanin’ acidic extraction method would serve our purpose, i.e. to screen rapidly whether a cell culture or regenerated plant produces VBL and/or VCR.…”

Section: Resultsmentioning

confidence: 99%

Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow‐injection electrospray ionization mass spectrometry

Favretto

Piovan

Filippini

et al. 2001

Rapid Comm Mass Spectrometry

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A semiquantitative determination of two bis-indole antitumor alkaloids, vincristine and vinblastine, has been performed by flow-injection electrospray ionization mass spectrometric analysis of the extracts of Catharanthus roseus. Leaves and flowers of two different phenotypes (pink flower and white flower) obtained from somatic embryogenesis were thus examined and compared with the field-grown mother plant. Different amounts of vincristine and vinblastine were detected depending on the examined samples.

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Section: Resultsmentioning

confidence: 99%

Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow‐injection electrospray ionization mass spectrometry

Favretto

Piovan

Filippini

et al. 2001

Rapid Comm Mass Spectrometry

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“…Recently, an ABC transporter, CrTPT2, whose primary function consists in enhancing the transport and hence, the accumulation of catharanthine in the leaf epidermal surface, has been identified [ 6 ]. However, the physical separation of catharanthine and vindoline observed by Yu and DeLuca [ 6 ] is probably a limiting factor in very young leaves, where AVLB was actually shown to be absent [ 8 ], but definitely not in developed leaves, where the dimer AVLB was repeatedly reported to be abundant [ 8 , 9 , 10 , 11 , 12 ]. In fact, Carqueijeiro et al [ 12 ] demonstrated that catharanthine, vindoline and AVLB were accumulated in the vacuoles of mesophyll cells by a specific proton antiport system, dependent on the transtonoplast pH gradient generated by V-H + -ATPase and V-H + -PPase using vacuoles isolated from leaves of adult plants.…”

Section: Introductionmentioning

confidence: 99%

Indole Alkaloids from Catharanthus roseus: Bioproduction and Their Effect on Human Health

2015

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Catharanthus roseus is a medicinal plant belonging to the family Apocynaceae which produces terpenoid indole alkaloids (TIAs) of high medicinal importance. Indeed, a number of activities like antidiabetic, bactericide and antihypertensive are linked to C. roseus. Nevertheless, the high added value of this plant is based on its enormous pharmaceutical interest, producing more than 130 TIAs, some of which exhibit strong pharmacological activities. The most striking biological activity investigated has been the antitumour effect of dimeric alkaloids such as anhydrovinblastine, vinblastine and vincristine which are already in pre-, clinical or in use. The great pharmacological importance of these indole alkaloids, contrasts with the small amounts of them found in this plant, making their extraction a very expensive process. To overcome this problem, researches have looked for alternative sources and strategies to produce them in higher amounts. In this sense, intensive research on the biosynthesis of TIAs and the regulation of their pathways has been developed with the aim to increase by biotechnological approaches, the production of these high added value compounds. This review is focused on the different strategies which improve TIA production, and in the analysis of the beneficial effects that these compounds exert on human health.

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“…α‐3′,4′‐Anhydrovinblastine (AVLB) is a dimeric monoterpenoid indole alkaloid present in Catharanthus roseus (L.) G. Don leaves [1–4], where it is believed to be the metabolic precursor of the anticancer drugs vinblastine (VLB) and vincristine (VCR) (Scheme. 1 ).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of α‐3′,4′‐anhydrovinblastine synthase (peroxidase‐like) from Catharanthus roseus (L.) G. Don

et al. 1998

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An H P O P -dependent enzyme capable of coupling catharanthine and vindoline into K K-3P,4P-anhydrovinblastine (AVLB) was purified to apparent homogeneity from Catharanthus roseus leaves. The enzyme shows a specific AVLB synthase activity of 1.8 nkat/mg, and a molecular weight of 45.40 kDa (SDS-PAGE). In addition to AVLB synthase activity, the purified enzyme shows peroxidase activity, and the VIS spectrum of the protein presents maxima at 404, 501 and 633 nm, indicating that it is a high spin ferric heme protein, belonging to the plant peroxidase superfamily. Kinetic studies revealed that both catharanthine and vindoline were substrates of the enzyme, AVLB being the major coupling product.z 1998 Federation of European Biochemical Societies.

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Extraction of 3′,4′-anhydrovinblastine from Catharanthus roseus

Cited by 25 publications

References 14 publications

Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow‐injection electrospray ionization mass spectrometry

Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow‐injection electrospray ionization mass spectrometry

Indole Alkaloids from Catharanthus roseus: Bioproduction and Their Effect on Human Health

Purification and characterization of α‐3′,4′‐anhydrovinblastine synthase (peroxidase‐like) from Catharanthus roseus (L.) G. Don

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