Extraction, isolation, immunoregulatory activity, and characterization of Alpiniae oxyphyllae fructus polysaccharides

Yang, Xin; Yang, Yuhui; Chen, Huricha; Xu, Teng; Li, Chengheng; Zhou, Ruigang; Gao, Linglin; Mengmeng, Han; He, Xiao-Tao; Chen, Yun

doi:10.1016/j.ijbiomac.2019.11.051

Cited by 17 publications

(12 citation statements)

References 44 publications

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“…The right shift phenomenon implied that the polarity of the microenvironment around Trp residues in α-glucosidase changed and became more hydrophilic after the addition of MHP [38]. Many studies showed that the polysaccharide with moderate molecular weight and high water solubility has the biological activity, while that with double helix or triple helix structure has better biological activity than other polysaccharides [14]. In the present study, MHP had a certain triple helix structure.…”

Section: In Vitro Antioxidant Activitymentioning

confidence: 54%

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Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from Malus hupehensis

Xue

Xiao

et al. 2021

Molecules

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Malus hupehensis (M. hupehensis), an edible and medicinal plant with significant antioxidant and hypoglycemic activity, has been applied to new resource foods. However, the structural characterization and biological effects of its polysaccharides (MHP) are less known. The optimum extraction parameters to achieve the highest extraction efficiency (47.63%), the yield (1.68%) and purity of MHP (89.6%) by ultrasonic-assisted aqueous two-phase system (ATPS) were obtained under the liquid-to-solid ratio of 23 g/mL, ultrasonic power of 65 W, and ultrasonic time of 33 min. According to the analysis results, MHP was composed of Man, GlcA, Rha, GalA, Glc, Gal, Xyl, Ara, and Fuc, in which Ara and Gal were the main components, and the content of GlcA was the lowest. In in vitro activity analysis, MHP showed a significant antioxidant capacity, and an inhibition activity of α-glucosidase and the advanced glycation end products (AGEs) formation in the BSA/Glc reaction model. MHP interacted with α-glucosidase and changed the internal microenvironment of the enzyme, and inhibited the AGEs formation, which provides more evidence for the antihyperglycemic mechanism of MHP. The results suggest that ATPS is an efficient and environmentally friendly solvent system, and M. hupehensis has broad application prospects in functional foods, healthcare products, and pharmaceuticals.

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Section: In Vitro Antioxidant Activitymentioning

confidence: 54%

“…The extraction and application of polysaccharides is the interesting research point of the medicine and biology field. The structural and biological properties of polysaccharides are closely related to their molecular weight, monosaccharide composition, and three-dimensional structures [14].…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from Malus hupehensis

Xue

Xiao

et al. 2021

Molecules

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“…Finally, the anion-exchange resins (D301 and D315) were respectively combined in series with the strongly basic anion-exchange resin and the strongly acidic cation-exchange resin column with the best screening effect. The extract of Jerusalem artichoke was separated by three ion-exchange columns. , …”

Section: Materials and Methodsmentioning

confidence: 99%

“…The extract of Jerusalem artichoke was separated by three ionexchange columns. 45,46 According to the determination method stipulated by the National Soft Drink Association (NSDA), the absorbance of the solution was determined at the wavelength of 420 and 720 nm, respectively. The actual color value A of the solution was the difference between A 420 and A 720 , and the decolorization rate of solution before and after resin treatment was defined as where A 420 and A 720 represent the absorbance of the solution measured at 420 and 720 nm, respectively.…”

Section: ■ Conclusionmentioning

confidence: 99%

Extraction and Purification of Inulin from Jerusalem Artichoke with Response Surface Method and Ion Exchange Resins

et al. 2022

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Inulin is used as an important food ingredient, widely used for its fiber content. In this study the operational extraction variables to obtain higher yields of inulin from Jerusalem artichoke tubers, as well as the optimal conditions, were studied. Response surface methodology and Box–Behnken design were used for optimization of extraction steps. The optimal extraction conditions were as follows: extraction temperature 74 °C, extraction time 65 min, and ratio of liquid to solid 4 mL/g. Furthermore, series connection of ion-exchange resins were used to purify the extraction solution where the optimal resin combinations were D202 strongly alkaline anion resin, HD-8 strongly acidic cation resin, and D315 weakly alkaline resin while the decolorization rate and decreased salinity reached 99.76 and 93.68, respectively. Under these conditions, the yield of inulin was 85.4 ± 0.5%.

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“…Alpiniae oxyphyllae fructus (AOF), which is the dry grown-up fruit of Alpinia oxyphylla Miq. Has a long history of application in traditional clinical Chinese medicine ( Yang et al, 2020 ). In traditional medicine, AOF has been widely utilized for treatment of emesis, diarrhea, frequent urination, spermatorrhea and so on ( Li R. et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated untargeted and targeted metabolomics to reveal therapeutic effect and mechanism of Alpiniae oxyphyllae fructus on Alzheimer’s disease in APP/PS1 mice

Zhou

Liu²,

Zhang³

et al. 2023

Front. Pharmacol.

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Introduction:Alpiniae oxyphyllae Fructus (AOF) has been abundantly utilized for the treatment of diarrhea, dyspepsia, kidney asthenia, and abdominal pain in China. AOF is effective for treating AD in clinical trials, but its exact mode of action is yet unknown.Methods: In this study, metabolomics was combined to ascertain the alterations in plasma metabolism in APP/PS1 transgenic mice, the therapy of AOF on model mice, and the dynamic variations in 15 bile acids (BAs) concentration.Results: 31 differential biomarkers were finally identified in APP/PS1 group vs. the WT group. The levels of 16 metabolites like sphinganine (Sa), lyso PE (20:2), lysoPC (17:0), glycocholic acid (GCA), deoxycholicacid (DCA) were increased in APP/PS1 group, and those of 15 metabolites like phytosphingosine, cer (d18:0/14:0), and fumaric acid were reduced in APP/PS1 group. After AOF treatment, 29 of the 31 differential metabolites showed a tendency to be back-regulated, and 15 metabolites were significantly back-regulated, including sphinganine (Sa), lyso PE (20:2), glycocholic acid (GCA), deoxycholic acid (DCA). The relationship between BAs level and AD had been received increasing attention in recent years, and we also found notable differences between DCA and GCA in different groups. Therefore, a BAs-targeted metabonomic way was established to determine the level of 15 bile acids in different groups. The consequence demonstrated that primary BAs (CA, CDCA) declined in APP/PS1 model mice. After 3 months of AOF administration, CA and CDCA levels showed an upward trend. Conjugated primary bile acids (TCA, GCA, TCDCA, GCDCA), and secondary bile acids (DCA, LCA, GDCA, TDCA, TLCA GLCA) ascended in APP/PS1 group. After 3 months of AOF treatment, the levels of most BAs decreased to varying degrees. Notably, the metabolic performance of DCA and GCA in different groups was consistent with the predictions of untargeted metabolomics, validating the correctness of untargeted metabolomics.Discussion: According to metabolic pathways of regulated metabolites, it was prompted that AOF ameliorated the symptom of AD mice probably by regulating bile acids metabolism. This study offers a solid foundation for further research into the AOF mechanism for the therapy of AD.

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Extraction, isolation, immunoregulatory activity, and characterization of Alpiniae oxyphyllae fructus polysaccharides

Cited by 17 publications

References 44 publications

Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from Malus hupehensis

Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from Malus hupehensis

Extraction and Purification of Inulin from Jerusalem Artichoke with Response Surface Method and Ion Exchange Resins

Integrated untargeted and targeted metabolomics to reveal therapeutic effect and mechanism of Alpiniae oxyphyllae fructus on Alzheimer’s disease in APP/PS1 mice

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