Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from Malus hupehensis

Abstract

Malus hupehensis (M. hupehensis), an edible and medicinal plant with significant antioxidant and hypoglycemic activity, has been applied to new resource foods. However, the structural characterization and biological effects of its polysaccharides (MHP) are less known. The optimum extraction parameters to achieve the highest extraction efficiency (47.63%), the yield (1.68%) and purity of MHP (89.6%) by ultrasonic-assisted aqueous two-phase system (ATPS) were obtained under the liquid-to-solid ratio of 23 g/mL, ultrasonic power of 65 W, and ultrasonic time of 33 min. According to the analysis results, MHP was composed of Man, GlcA, Rha, GalA, Glc, Gal, Xyl, Ara, and Fuc, in which Ara and Gal were the main components, and the content of GlcA was the lowest. In in vitro activity analysis, MHP showed a significant antioxidant capacity, and an inhibition activity of α-glucosidase and the advanced glycation end products (AGEs) formation in the BSA/Glc reaction model. MHP interacted with α-glucosidase and changed the internal microenvironment of the enzyme, and inhibited the AGEs formation, which provides more evidence for the antihyperglycemic mechanism of MHP. The results suggest that ATPS is an efficient and environmentally friendly solvent system, and M. hupehensis has broad application prospects in functional foods, healthcare products, and pharmaceuticals.

Optimization of ultrasound‐assisted aqueous two‐phase extraction of polysaccharides from seabuckthorn fruits using response methodology, physicochemical characterization and bioactivities

“…2(a), when the other factors (extraction time of 30 min and liquid‐to‐material ratio of 60:1) were fixed, the maximum yield of 31.17 ± 0.04% was gained at the ultrasonic power of 400 W. This phenomenon may be explained that the proper ultrasonic power promotes the fragmentation of plant tissue and increases the solubility of the polysaccharide. Once the power density surpasses a certain range, the polysaccharides will be easily denatured and degraded 16 . Furthermore, when the ultrasonic power (400 W) and liquid‐to‐material ratio (60 mL/g) were kept constant, SP yield reached the maximum (32.61 ± 0.14%) at 40 min and then declined above 40 min (Fig.…”

“…It can be seen from Figure 5(a) that the absorption peaks at 3421, 2923, 1748, and 1617 cm −1 belonged to the stretching vibrations of O–H, C–H, CO and COO − 41 . However, the intensities of peaks at 1748, 1617 and 1441 cm −1 in PUSP were weaker than those in PLSP, which was due to the relatively low uronic acid content in PUSP 16,27 . The absorption peak at 1102 cm −1 indicated the presence of the pyranose ring 18,27 .…”

“…In particular, ATPS of ethanol/salt aqueous has attracted a lot of attention in extracting polysaccharides from plants owing to low viscosity, easy demixing and solvent recycling 14,15 . Based on the biphasic extraction property, the upper‐phase and the lower‐phase polysaccharides can be extracted at the same time from the sample using ATPE 10,14,16 …”

A comprehensive perspective of traditional Arabic or Islamic medicinal plants as an adjuvant therapy against COVID-19

“…Aloe vera, xxxx , Assessment of phytochemical, xxxx , E-Flora, xxxx , Fenugreek, xxxx , Ficus carica, xxxx , Inhibition, xxxx , Khan, J, xxxx , Li et al, 2021a , Li et al, 2021b , Medail et al, 2001 , Advances, 2013 , Tae Velthuis et al, 2010 , Wang et al, 2020a , Wang et al, 2020b , World Health Organization (WHO), 2005 , Zhou et al, 2020a , Zhou et al, 2020b .…”

Bioactive Substances and Biological Functions in Malus hupehensis: A Review

“…The results show that the monosaccharides of MH consisted of Man (3.22%), GlcA (1.85%), Rha (4.25%), GalA (5.08%), Glc (6.69%), Gal (38.46%), Xyl (4.68%), Ara (30.90%), and Fuc (4.87%). The results of Congo red test and circular dichroism show that the polysaccharide of MH had asymmetric and triple helix structure [ 29 ]. It is worth noting that there are relatively few studies on the polysaccharides of MH.…”