Extraction, isolation, characterization and antioxidant activity of polysaccharides from Astragalus membranaceus

Preliminary characterization and immunostimulatory activity of a novel functional polysaccharide from Astragalus residue fermented by Paecilomyces sinensis

“…1 Astragalus polysaccharides (APS) have various important biological activities such as anti-oxidant activity, immunomodulatory activity, anti-tumor activity, antidiabetes activity, antiviral activity, hepatoprotection activity, and anti-inammation activity. [2][3][4][5][6][7][8][9][10] In industrial production, most methods used to extract APS involve water extraction and alcohol precipitation. These methods not only leave many other bioactive substances behind, but also produce a large amount of Astragalus residue.…”

Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes

“…On the basis of the results of the single-factor studies, the following conditions were employed for later orthogonal and BBD experiments: pectinase amount of 15-25 g/kg, papain and cellulase amounts of 10-20 g/kg, pH of 4.0-6.0, temperature of 40-60°C, enzymatic treatment time of 70-110 min and liquid/material ratio of 20:1-40:1 mL/g.3.2 Orthogonal analysis of complex enzyme-assisted extractionPlant cell walls are composed of cellulose, hemi-cellulose and pectin, which are barriers for the release of intracellular material from the cells[25]. Therefore, certain enzymes, such as cellulose, papain and pectinase are often used to increase the recovery by breaking cell walls and hydrolyzing structural polysaccharides[26][27][28]. To reduce the number of experimental trials and understand the interactions between factors, an orthogonal test design was employed to optimize the compound enzyme amounts.…”

Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

“…Credibly, the physical and sonochemical effects of ultrasound treatment have been reported to improve the functionality of the sonicated extracts compared to other traditional extraction methods (Vilkhu, Mawson, Simons, & Bates, ). To reinforce this assertion, Chen et al () reported that APS extracted by the reflux method had a relatively weaker scavenging activity on the ˙OH radical compared to the APS extracts isolated using the combined enzyme method. Likewise, combined Astragalus and Angelica polysaccharides extracted using the hot water method were reported to have moderate effects on the hydroxyl radical (Pu et al, ).…”