Extraction, GC-MS Evaluation and Anti-epileptic Potential of Seeds Ethanolic Extract of Putranjiva roxburghii Wall

Abstract

Background: Putranjiva roxburghii Wall is traditionally known to cure many pathological conditions including epilepsy. Objective: The present study is aimed at determining bioactive compounds in ethanolic extract of Putranjiva roxburghii test extract (PRTE) seeds by GCMS analysis and to assess its anti-epileptic potential using various experimental models of epilepsy. Methods: The ethanolic extract of seeds of Putranjiva roxburghiiwas subjected to GC-MS analysis to detect the bioactive phytoconstituents. Acute oral toxicity of the extract was performed using OCED guideline 420. Pentylenetetrazol (PTZ) kindling model of epilepsy and Maximal electroshock epilepsy (MES) model of epilepsy were used to determine antiepileptic potential. Results: The GC-MS analysis of the extract revealed the presence of 20 phytoconstituents. The major phytoconstituents included n-Propyl heptyl ether (25.25%), 5-Ethyl hydantoin (8%), octadec-9-enoic acid (16-25%) and 1, 2-Benzene dicarboxylic acid (11.86%). The PRTE (50 mg/kg and 100 mg/kg) afforded significant and dose - dependent protection against PTZ – induced kindling epilepsy and MES induced epilepsy (p<0.001 and p<0.01). Conclusion: Based on the above finding, it is evident that Putranjiva roxburghii seeds contain biologically active compound. It can also be concluded that the extract possesses anti-epileptic potential.

Drug Delivery of Natural Products Through Nanocarriers for Effective Breast Cancer Therapy: A Comprehensive Review of Literature

“… 192 More recent studies have revealed the anti-BC, anti-epileptic, antioxidant, anti-inflammatory, antimicrobial, anti-nociceptive and anti-pyretic potentials of P. roxburghii leaf and seed extracts. 193–195 However, further mechanistic studies are required to explain how P. roxburghii extracts exert these pharmacological activities.…”

Recent Advances in the Synthesis of Isothiocyanates Using Elemental Sulfur

“…Besides the well-known trapping of (hetero)cyclic carbenes with sulfur, the transformation of di-or trihalogenated compounds to halocarbenes and the following reaction with sulfur are considered as a convenient process [49][50][51]. Common halogenated reagents are chloroform (6), trimethyl(trifluoromethyl)silane (F3CSiMe3, 7) and the sodium oxides leads to many by-products, rendering this approach less attractive [31]. The reaction of isocyanides with disulfides in the presence of thallium (I)-salts as catalysts also leads to the formation of ITCs [32].…”

“…Besides the well-known trapping of (hetero)cyclic carbenes with sulfur, the transformation of di-or trihalogenated compounds to halocarbenes and the following reaction with sulfur are considered as a convenient process [49][50][51]. Common halogenated reagents are chloroform (6), trimethyl(trifluoromethyl)silane (F 3 CSiMe 3 , 7) and the sodium and potassium salts of chlorodifluoroacetic acid (e.g., ClF 2 CCO 2 Na, 8) and bromodifluoroacetic acid (e.g., BrF 2 CCO 2 K, 9, Scheme 3) [52]. Notably, (triphenylphosphonio)difluoroacetate (PDFA, 10), prepared from BrF 2 CCO 2 K with triphenylphosphine, is a more efficient precursor of difluorocarbene based on the research work of Xiao and co-workers [53,54].…”

“…They are represented among natural products and pharmaceutical ingredients by the biologically relevant welwitindolinone and hapalindole alkaloids isolated from various algae species [4]. Notably, glucosinolates, found as secondary metabolites in almost all plants, contain the -S-C=N-functional group and act as a precursor for various ITCs [5,6]. Tissue damage of the plant promotes myrosinase enzyme activity as a defence mechanism, triggering the degradation of glucosinolates, and releasing, e.g., allyl, benzyl or phenethyl ITC or sulforaphane [7].…”