Extraction, Enrichment, and <i>in situ</i> Electrochemical Detection on Lab-on-a-Disc: Monitoring the Production of a Bacterial Secondary Metabolite

Andreasen, Sune Zoëga; Sanger, Kuldeep; Jendresen, Christian Bille; Nielsen, Alex Toftgaard; Emnéus, Jenny; Boisen, Anja; Zór, Kinga

doi:10.1021/acssensors.8b01277

Cited by 20 publications

(18 citation statements)

References 62 publications

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“…Based on enhancing the sensitivity and selectivity of electrochemical pathogen sensors, researchers have focused on the design of functional integrated platforms and portable devices. Microfluidic device has integrated pretreatment unit, capture unit and detection unit [27,28]. There is no doubt that the use of microfluidic devices simplifies the actual sample pretreatment process.…”

Section: Recognition Elements and Electrochemical Methods‐based Sensorsmentioning

confidence: 99%

“…(C) Illustration of custom‐designed MiSens biosensor integrating microfluidic system, biochip design and real‐time amperometry [27]. (D) Inner diagram of the lab‐on‐a‐disc sensor with integrated supported liquid membrane (SLM) extraction and embedded electrodes for detection; a) Illustrative diagram of the donor unit and b) acceptor‐detection unit; c) pHCA becomes neutral, while Tyr (existing basic amino group) is positively charged in the acceptor unit; d) During the extraction process, neutral pHCA diffuses to the SLM to the acceptor site, where it becomes negatively charged [28]. (E) Photoelectrochemical system for capture, detection, and inactivation of E. coli [36].…”

Section: Electrochemical Sensors For Detection Of Foodborne Pathogeni...mentioning

confidence: 99%

“…Genetically modified E. coli expressing tyrosine (Tyr) ammonia‐lyase could convert Tyr to hydroxycinnamic acid (pHCA). In order to realize efficient detection of pHCA in complex samples, there was a centrifugal fluidic platform consisting of supported liquid membrane extraction and SWV detection (Figure 1D) [28]. Although this method is beneficial for online monitoring of bacterial bioprocess, it can only test single sample in a small volume (3 μL), which requires the modification of nanomaterials on the electrode to improve the sensitivity of the sensing system.…”

Section: Electrochemical Sensors For Detection Of Foodborne Pathogeni...mentioning

confidence: 99%

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Perspectives for Recognition and Rapid Detection of Foodborne Pathogenic Bacteria Based on Electrochemical Sensors

Wang¹,

Ma²,

Qiao³

et al. 2021

eFood

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Due to the misuse of pathogen-infected food, human beings continue to face serious diseases and even deaths. Thus, researchers from all walks of life concern on prevention, detection, and resistance for pathogenic bacteria. It is necessary to develop and explore a fast, cost-effective, portable, and efficient detection technology for pathogens. Recently, electrochemical sensors have been widely used for food safety analysis, due to their increasingly high sensitivity and selectivity. In particular, researchers have made significant breakthroughs on signal enhancement strategies, quantitative methods, and miniaturization of the instrument, which can be used as a reference to food safety analysis. Moreover, a device integrating facile working procedures, miniaturization, and automation must be indispensable. In order to meet the needs of People's Daily life, household device and point-of-care (POC) testing need to be combined with electrochemical sensing technology. In this review, the state of the art in electrochemical sensing for recognition and rapid detection of foodborne pathogenic bacteria is summarized from perspectives of common foodborne pathogens, especially focusing on real-time monitoring, lab-on-a-chip, and photoelectrochemical (PEC) platform applied in food and medical treatment fields. Furthermore, the limitations and prospects of recognition elements, active nanomaterials, and optical materials, that are essential components in sensing systems on future research directions, were evaluated.

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Section: Recognition Elements and Electrochemical Methods‐based Sensorsmentioning

confidence: 99%

Section: Electrochemical Sensors For Detection Of Foodborne Pathogeni...mentioning

confidence: 99%

Section: Electrochemical Sensors For Detection Of Foodborne Pathogeni...mentioning

confidence: 99%

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Perspectives for Recognition and Rapid Detection of Foodborne Pathogenic Bacteria Based on Electrochemical Sensors

Wang¹,

Ma²,

Qiao³

et al. 2021

eFood

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“…Applications can include high resolution imaging of cells [36] and facilitating sensing and automated / on-line measurement and biosensing [9] . Biosensing technologies which have been used, include fluorescence-based detection [37] , surface plasmon resonance [38] , absorbance [11] , [39] , and electrochemistry [40] . Recently, with the reduction in cost of electronic microprocessors, there is a growing trend towards developing customised systems which can rotate with the disc during sensing.…”

Section: Hardware In Contextmentioning

confidence: 99%

Design and fabrication of a low-cost wireless camera imaging system for centrifugal microfluidics

et al. 2022

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Centrifugal microfluidic devices offer a robust method for low-volume fluid handling by combining low-cost instrumentation with highly integrated automation. Crucial to the efficacy of Lab-on-a-Disc (LoaD) device operation is the selection of robust valving technology, the design of on-disc fluidic structures, and accurate control of disc spin-speeds (centrifugal force) during operation. The design and refinement of fluidic and valving structures is often guided by inspecting disc operation using high-speed camera systems. This approach involves synchronising image acquisition with disc rotation to visualise liquid flow through a series of images often presented in a video format. Depending on the decisions taken, such systems can cost from €4,000 upwards. This paper outlines the development of a low-cost centrifugal test-stand with an integrated imaging system using a generic wireless camera to record videos directly to a smartphone device. This imaging system can be fabricated using only 3D printers and a low-cost CNC milling machine from widely available materials for approximately €350. High-fidelity imaging of the entire disc for flow visualisation and the recording of real-time colour intensity measurements are facilitated by this standalone device. A vibration analysis study has been performed to determine the rotational velocity range at which the system can be safely operated. Furthermore, the efficacy of the imaging system has been demonstrated by performing real-time colour intensity measurements of dyed water dilutions.

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“…All static measurements were performed in open vial LoD systems ( Figure S2). An open vial LoD system can be used for electrochemical characterization as well as for calibration 54 . SWV is a well-accepted voltammetric technique for quantitative measurements 57 .…”

Section: Figure 3 (A)mentioning

confidence: 99%

Modular, Lightweight, Wireless Potentiostat-on-a-Disc for Electrochemical Detection in Centrifugal Microfluidics

et al. 2019

Self Cite

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Interfacing electrochemical sensors in lab-on-a-disc (LoD) system with a potentiostat is often tedious and challenging. We here present the first multichannel, modular, lightweight and wirelessly powered, custom-built potentiostat-on-a-disc (PoD) for centrifugal microfluidic applications. The developed potentiostat is in the form factor of a typical DVD and weighs only 127 g. The design of the potentiostat facilitates easy and robust interfacing with the electrodes in the LoD system, while enabling real-time electrochemical detection during rotation. The device can perform different electroanalytical techniques such as cyclic voltammetry, square wave voltammetry, amperometry while being controlled by custom-made software. Measurements were conducted with and without rotation using both inhouse fabricated and commercial electrodes. The performance of the PoD was in good agreement with the results obtained using a commercial potentiostat with a measured current resolution of 200 pA. As a proof-of-concept, we performed a real-time release study of an electrochemically active compound from microdevices used for drug delivery.

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Extraction, Enrichment, and in situ Electrochemical Detection on Lab-on-a-Disc: Monitoring the Production of a Bacterial Secondary Metabolite

Cited by 20 publications

References 62 publications

Perspectives for Recognition and Rapid Detection of Foodborne Pathogenic Bacteria Based on Electrochemical Sensors

Perspectives for Recognition and Rapid Detection of Foodborne Pathogenic Bacteria Based on Electrochemical Sensors

Design and fabrication of a low-cost wireless camera imaging system for centrifugal microfluidics

Modular, Lightweight, Wireless Potentiostat-on-a-Disc for Electrochemical Detection in Centrifugal Microfluidics

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