Reusability
of sensors is relevant when aiming to decrease variation
between measurements, as well as cost and time of analysis. We present
an electrochemically assisted surface-enhanced Raman spectroscopy
(SERS) platform with the capability to reverse the analyte–surface
interaction, without damaging the SERS substrate, allowing for efficient
sensor reuse. The platform was used in combination with a sample pretreatment
step, when detecting melamine from milk. We found that the electrochemically
enhanced analyte–surface interaction results in significant
improvement in detection sensitivity, with detection limits (0.01
ppm in PBS and 0.3 ppm in milk) below the maximum allowed levels in
food samples. The reversibility of interaction enabled continuous
measurement in aqueous solution and a complete quantitative assay
on a single SERS substrate.
Interfacing electrochemical sensors in lab-on-a-disc (LoD) system with a potentiostat is often tedious and challenging. We here present the first multichannel, modular, lightweight and wirelessly powered, custom-built potentiostat-on-a-disc (PoD) for centrifugal microfluidic applications. The developed potentiostat is in the form factor of a typical DVD and weighs only 127 g. The design of the potentiostat facilitates easy and robust interfacing with the electrodes in the LoD system, while enabling real-time electrochemical detection during rotation. The device can perform different electroanalytical techniques such as cyclic voltammetry, square wave voltammetry, amperometry while being controlled by custom-made software. Measurements were conducted with and without rotation using both inhouse fabricated and commercial electrodes. The performance of the PoD was in good agreement with the results obtained using a commercial potentiostat with a measured current resolution of 200 pA. As a proof-of-concept, we performed a real-time release study of an electrochemically active compound from microdevices used for drug delivery.
Therapeutic drug monitoring (TDM) is an essential clinical practice for optimizing drug dosing, thereby preventing adverse effects of drugs with a narrow therapeutic window, slow clearance, or high interperson pharmacokinetic variability. Monitoring methotrexate (MTX) during high-dose MTX (HD-MTX) therapy is necessary to avoid potentially fatal side effects caused by delayed elimination. Despite the efficacy of HD-MTX treatment, its clinical application in resource-limited settings is constrained due to the relatively high cost and time of analysis with conventional analysis methods. In this work, we developed (i) an electrochemically assisted surface-enhanced Raman spectroscopy (SERS) method for detecting MTX in human serum at a clinically relevant concentration range and (ii) a benchtop, Raman detection system with an integrated potentiostat, software, and data analysis unit that enables mapping of small areas of SERS substrates and quantitative SERS-based analysis. In the assay, by promoting electrostatic attraction between gold-coated nanopillar SERS substrates and MTX molecules in aqueous samples, a detection limit of 0.13 μM with a linear range of 0.43−2 μM was achieved in PBS. The implemented sample cleanup through gel filtration proved to be highly effective, resulting in a similar detection limit (0.55 μM) and linear range (1.81−5 μM) for both PBS and serum. The developed and optimized assay could also be used on the in-house built, Raman device. We showed that MTX detection can be carried out in less than 30 min with the Raman device, paving the way toward the TDM of MTX at the pointof-need and in resource-limited environments.
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