Extraction and structural characteristics of pectic polysaccharides from Abies sibirica L

Shakhmatov, Evgeny G.; Udoratina, E. V.; Atukmaev, Konstantin V.; Makarova, Elena N.

doi:10.1016/j.carbpol.2015.01.041

Cited by 40 publications

(12 citation statements)

References 39 publications

(19 reference statements)

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“…In addition, some weak cross-peaks at 97/5.1, 72/3.4, 73/3.7, 78/4.0, and 49/3.4 ppm, which can be assigned to C1-H1, C2-H2, C3-H3, C4-H4, C5-H5, and -OCH3 of 4-O-Me-α-D-GlcpA units (Sun et al 2014;Peng and She 2014), were also detected in the HSQC spectrum. The peaks at C1-H1 of D-GalpA residues at 97/5.1 were shifted compared to that usually reported at 101.5/5.1 (Shakhmatov et al 2015). Although there was no clear evidence of protein attachment, it is suggested that there might be a connection between proteins and D-GlapA residues.…”

Section: And 2d Nmr Spectracontrasting

confidence: 56%

Fractional Isolation and Structural Characterization of Hemicelluloses from Soybean Hull

Wang¹,

Li²,

Liu³

et al. 2015

BioResources

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Hemicellulosic fractions were extracted from soybean hull with various concentrations of NaOH at 50 °C for 5 h. The chemical compositions and physicochemical properties were determined by high performance anion exchange chromatography (HPAEC), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analyzer (TGA), and 13 C and 2D nuclear magnetic resonance (NMR) analysis. The crystallinity of soybean hulls treated and untreated by alkali liquid was examined by X-ray diffraction (XRD). The sugar analysis results indicated that all of the hemicellulosic preparations were heteropolysaccharides containing arabinose, galactose, glucose, xylose, and mannose. According to the spectral analysis, hemicelluloses from soybean hull were assumed to be L-arabino-4-O-methyl-D-glucurono-D-xylan. Xylose was the predominant monosaccharide in the hemicellulose fraction 1 (H1), and it ranged between 40.1% and 48.8% of the total neutral sugars. It was found that hemicellulose fraction 3 (H3) had the highest thermal stability and H1 had the lowest thermal stability. The crystallinity index (CrI) was found to be about 20.8%, 14.3%, 10.9%, and 4.7% for soybean hulls, untreated and treated with 1.5, 2.0, and 2.5 M NaOH, respectively.

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Section: And 2d Nmr Spectracontrasting

confidence: 56%

Fractional Isolation and Structural Characterization of Hemicelluloses from Soybean Hull

Wang¹,

Li²,

Liu³

et al. 2015

BioResources

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“…The Ara present in both polymers are linked both 1,5; 1,3,5 and 1,2,3,5, indicating high branching on the part of the polymer rich in Ara, especially in GCP-II-I. These results indicated the Ara present in both fractions are a highly branched 1, 5-arabinan (Shakhmatov, Udoratina, & Atukaev, 2015;Shakhmatov, Toukach, Michailowa, & Makarova, 2014;Kang, Cui, Phillips, Chen, Guo, & Wang. 2011).…”

Section: Determination Of the Glycosidic Linkagesmentioning

confidence: 67%

“…The polysaccharide fractions GCP-I-I and GCP-II-I were characterized by 1D NMR spectroscopy and compared with chemical shift values from literature (Chai & Zhao, 2016;Hromádková, Košt'álová, Vrchotová, & Ebringerová, 2014;Košt'álová, Hromádková, & Ebringerová, 2013;Li, Fan, & Ding, 2011;Zou et al, 2014). Fraction GCP-II-I had somewhat better solubility than fraction GCP-I-I, due to lower Mw (Austarheim, et al, 2012b;Shakhmatov, et al, 2015) and…”

Section: Structural Featuresmentioning

confidence: 96%

Polysaccharides with immunomodulating activity from roots of Gentiana crassicaulis

Zou

Chen

et al. 2017

Carbohydrate Polymers

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“…The degree of acetylation (DA) in polysaccharides was assayed by applying a combination of 1 H NMR and the method described by Tomoda et al [3,24,25]. According to the latter method, DNP-W6 (35 mg) was reacted with 0.3 mol/L NaOH (30 mL) at room temperature for 2 h. The solution was neutralized with HCl and dialyzed against distilled water.…”

Section: De-acetylation and Determination Of O-acetyl Groupsmentioning

confidence: 99%

“…The chemical structures can be divided into three main types: homogalacturonan (HG), rhamnogalacturonan-I (RG-I) and rhamnogalacturonan-II (RG-II) [2][3][4][5]. HG is a linear chain of (1!4)-a-D-galacturonic acid residues with some of the carboxyl groups methyl esterified [3]. RG-I contains a backbone of the repeating disaccharide [!4)-a-GalAp-(1!2)-a-Rhap-(1!]…”

Section: Introductionmentioning

confidence: 99%

Structure elucidation of a pectin fromDendrobium nobileLindl. and its immunological activity

Wang

Zhang

Luo

2018

Biotechnology & Biotechnological Equipment

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In this study, an RG-I pectin DNP-W6 was isolated from the stems of Dendrobium nobile Lindl. (Orchidaceae). It contained mannose, glucose, galactose, galacturonic acid and rhamnose in a molar ratio of 0.6: 1.0: 1.5: 1.1: 1.0. Its structural features were revealed by chemical, physical and spectral analysis. The results suggested that DNP-W6 possessed a backbone of a disaccharide of !4)-a-GalAp-(1!2)-a-Rhap-(1!, with approximate 50% substitution at O-4 of the rhamnopyranosyl residues. The side chains were neutral chains including galactosyl, mannosyl and glucosyl chains. The acetyl content was estimated to be 8.9%. The immunological results indicated that DNP-W6 and its derivatives exhibited different immunological activities in vitro, showing that the side chains and acetyl groups had an important effect on the expression of immunological activities.

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Extraction and structural characteristics of pectic polysaccharides from Abies sibirica L

Cited by 40 publications

References 39 publications

Fractional Isolation and Structural Characterization of Hemicelluloses from Soybean Hull

Fractional Isolation and Structural Characterization of Hemicelluloses from Soybean Hull

Polysaccharides with immunomodulating activity from roots of Gentiana crassicaulis

Structure elucidation of a pectin fromDendrobium nobileLindl. and its immunological activity

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