“…Various analytical methods and reagents was used for mercury determination that includes colorimetry, atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), cold vapour atomic absorption spectroscopy (CVAAS), cold vapour atomic fluorescence spectroscopy (CVAFS), polarography, neutron activation analysis, gas chromatography (GC) and high-performance liquid chromatography (HPLC) [8][9][10][11][12][13][14][15][16][17] . Similarly, reagents like 1-(1H-benzimidazol-2-yl) guanidine (GIB), (Sensing of mercury(II) using 1-(1H-benzimidazol-2-yl) guanidine as chromophore 18 , Poly(2-aminothiazole) (Synthesis of Poly(2-aminothiazole) for Selective Removal of Hg(II) in Aqueous Solutions 19 , 2-octylaminopyridine (2-OAP), Extraction and separation of mercury(II) from succinate media with high molecular weight amine as an extractant 20 , have been explored recent time. Due to increasing recognition that alternative medicine such as Ayurveda, Unani, and Homeopathy etc.…”