Extraction and Purification of Pectin from Agro-Industrial Wastes

Venkatanagaraju, Erumalla; Bharathi, N.; Sindhuja, Rachiraju Hema; Chowdhury, Rajshree Roy; Sreelekha, Yarram

doi:10.5772/intechopen.85585

Cited by 20 publications

(16 citation statements)

References 82 publications

(116 reference statements)

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“…The growing requirements of pectin in pharmaceutical applications have accelerated the need for competent extraction processes. Commercially, these naturally occurring biomolecules can be extracted from the peels of citrus fruits by alcohol precipitation (149). In the extraction process, the recovery of pectins is vital at the industrial scale to endow with passable supply for the increasing need.…”

Section: Extraction and Purification Methods Of Pectinmentioning

confidence: 99%

“…These advanced methods exhibit a large handling capacity, lower processing time, higher purity, a mild condition, and an energy-efficient and environment-friendly way of operation (150). However, for efficient purification, proper precipitation is required, which was achieved through alcohol precipitation with washing (149). Growing, investigation proved that the purification of pectins is efficiently attained through stepwise innovative methods like ethanol precipitation, ultrafiltration in combination with diafiltration, ultrafiltration, and metal precipitation (153).…”

Section: Extraction and Purification Methods Of Pectinmentioning

confidence: 99%

See 1 more Smart Citation

Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing

et al. 2020

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Both pectin and pectinase are vitally imperative biomolecules in the biotechnological sector. These molecules are a feasible non-toxic contrivance of nature with extensive applicative perception. Understanding pectic substances and their structure, unique depolymerization, and biochemical properties such as a catalytic mechanism and the strong interrelationship among these molecules could immensely enhance their applicability in industries. For instance, gaining knowledge with respect to the versatile molecular heterogeneity of the compounds could be considered as the center of concern to resolve the industrial issues from multiple aspects. In the present review, an effort has been made to orchestrate the fundamental information related to structure, depolymerization characteristics, and classification of pectin as well as the types and biochemical properties of pectinase. Furthermore, various production methods related to the optimization of the product and its significant contribution to the pharmaceutical industry (either pectinase or derived pectic substances) are described in this article.

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Section: Extraction and Purification Methods Of Pectinmentioning

confidence: 99%

Section: Extraction and Purification Methods Of Pectinmentioning

confidence: 99%

Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing

et al. 2020

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“…Hemicellulose is the second most abundant component of lignocellulosic biomass, after cellulose [ 61 , 62 ], and is found in blackcurrant pomace (constituting 25%), chokeberry (34%), cherry (11%), citrus peel (10%), and grapefruit waste (6%) [ 60 , 63 ]. Pectin is mainly found in fruit and vegetable peels, constituting 8–53% of orange peel, 1–17% of lemon peel, and 2–16% of grape skin; these amounts vary according to the different analytical and extraction methods [ 64 ].…”

Section: Main By-product Componentsmentioning

confidence: 99%

Interaction between Dietary Fibre and Bioactive Compounds in Plant By-Products: Impact on Bioaccessibility and Bioavailability

2023

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In Europe, around 31 million tonnes of food by-products are generated during primary production and trade. The management of these by-products may cause a negative impact, both at the economic and environmental levels, for both industry and society. In this regard, taking into consideration that these by-products retain the dietary fibre compositions and the bioactive compounds of the starting materials, plant food agro-industries have an interest in taking advantage of them, from a nutritional point of view. Therefore, this review evaluates the role of dietary fibre and bioactive compounds in these by-products as well as the potential interactions of both components and their implications for health, since the bioactive compounds associated with fibre may reach the colon, where they can be metabolised into postbiotic compounds, providing important health benefits (prebiotic, antioxidant, anti-inflammatory, etc.). Consequently, this aspect, on which there are few studies, is very relevant and must be considered in the revaluation of by-products to obtain new ingredients for food processing with improved nutritional and technological properties.

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“…Cellulases and hemicellulases are therefore crucial for the effective saccharification of these residues and the creation of free sugars from them. However, in addition to cellulose, hemicellulose, and lignin, other agro-industrial residues such as wheat bran, fruit peels, vegetable waste, rice bran, deoiled rice bran, maize bran, apple pomace, and tomato pomace also contain starch and pectin [56][57][58]. As a result, amylases and pectinases are necessary for the effective hydrolysis of these biomass residues.…”

Section: Microbial Enzymes Involved In the Hydrolysis Of Feedstocks F...mentioning

confidence: 99%

Microbial Enzyme Systems in the Production of Second Generation Bioethanol

Soni¹,

Sharma²,

Soni³

2022

Preprint

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The primary contributor to global warming has been the careless usage of fossil fuels. Urbanization's threat to the depletion of these resources has made it necessary to find alternatives due to the rising demand. Four different forms of biofuels are now available and constitute a possible replacement for fossil fuels. The first generation of biofuels is generated from the edible portion of biomass, the second generation is made from the non-edible portion of biomass, the third generation is made from algal biomass, and the fourth generation is made using molecular biology to improve the algal strain. Second generation biofuels are extremely important because they are derived from non-edible biomass, such as agricultural and agro-industrial wastes rich in cellulose, hemicellulose, pectin, and starch impregnated with lignin, and are hydrolyzed after delignification by physio-chemical or biological pretreatments using ligninases. There has been a lot of research on their production using chemical and enzymatic hydrolysis, but it has not been economically viable in comparison to first generation biofuels due to the formation of several inhibitors with chemical hydrolysis and the high cost of the enzymes. Furthermore, the need for multiple enzymes due to the various types of carbohydrates in the feedstocks makes the enzymatic process too expensive. This article examines the enzymes involved in the hydrolysis of feedstocks for the production of second generation bioethanol, a highly acceptable biofuel.

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Extraction and Purification of Pectin from Agro-Industrial Wastes

Cited by 20 publications

References 82 publications

Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing

Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing

Interaction between Dietary Fibre and Bioactive Compounds in Plant By-Products: Impact on Bioaccessibility and Bioavailability

Microbial Enzyme Systems in the Production of Second Generation Bioethanol

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