Extraction and identification of bioactive compounds (eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf

Ahsan, Taswar; Chen, Jianguang; Zhao, Xing‐Ming; Irfan, Muhammad; Wu, Yuanhua

doi:10.1186/s13568-017-0351-z

Cited by 130 publications

(73 citation statements)

References 28 publications

(27 reference statements)

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“…This increased lipase activity could correlate with the enhanced production of eicosane, perillic aldehyde and octadecanal as highly lipophilic molecules since Malassezia requires specific exogenous lipids for growth. Eicosane is reported as being produced by Streptomyces as an antifungal agent (22) which also supports its increased presence on the skin of PD sufferers.…”

Section: Supplementary Information Andmentioning

confidence: 68%

Discovery of volatile biomarkers of Parkinson’s disease from sebum

Trivedi

Sinclair

et al. 2018

Preprint

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Parkinson's disease (PD) is a progressive, neurodegenerative disease that presents with significant motor symptoms, for which there is no diagnostic test (1-3). We have serendipitously identified a hyperosmic individual, a 'Super Smeller' that can detect PD by odor alone, and our early pilot studies have indicated that the odor was present in the sebum from the skin of PD subjects(4). Here, we have employed an unbiased approach to investigate the volatile metabolites of sebum samples obtained noninvasively from the upper back of 64 participants in total (21 controls and 43 PD subjects). Our results, validated by an independent cohort, identified a distinct volatiles-associated signature of PD, including altered levels of perillic aldehyde and eicosane, the smell of which was then described as being highly similar to the scent of PD by our 'Super Smeller'.Physicians in ancient times, including Hippocrates, Galenus, and Avicenna, used odor as a diagnostic tool. Although the olfactory skills of physicians are not routinely used in modern medicine, it is well documented that a number of conditions, predominantly metabolic and infectious diseases, are associated with a unique odor(5), but there is scant evidence for odors as symptoms of neurodegenerative disorders. Joy Milne, a Super Smeller whose Recently, TD-GC-MS has been used as a volatilome analysis platform for the detection of compounds from bacteria implicated in ventilator associated pneumonia(12), for differentiation between odors due to human and animal decomposition(17), as well as aerosol detection of the fumes from e-cigarettes(18). This versatility of TD-GC-MS for samples from many sources renders it highly suitable for use in identifying the metabolites

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Section: Supplementary Information Andmentioning

confidence: 68%

Discovery of volatile biomarkers of Parkinson’s disease from sebum

Trivedi

Sinclair

et al. 2018

Preprint

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“…Earlier bioactive compounds like tannins from L. tridentate and F. cernua (Castillo et al 2010); Daturilin extracted from the leaves of Datura metel (Kagale et al, 2011) is reported to be active against pathogen R. solani . Another bioactive compound eicosane and dibutyl phthalate isolated from microbial species Streptomyces strain KX852460 reported as a potential bio-control agent for R. solani (Ahsan et al 2017).…”

Section: Discussionmentioning

confidence: 99%

A novel bioactive molecule 5-β-Hydroxyltridecanyl benzoate identified in Pedalium Murex L. (Gokhru) fruit extracts showed antifungal activity against Sclerotinia sclerotiorum

Chaudhary¹,

Kaushik

Reddy³

2018

Preprint

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Fruits of Pedalium murex known as, ‘Gokhru’ is predominantly used in Ayurveda for its aphrodisiac properties and use in treatment of urological orders. However till now, bioactivity of this fruit has not been documented for plant protection against phytopathogenes, Hence in this regard, we investigated antimicrobial activity of P. murex fruit extracts (isolated from the different agro climatic zones of India) were tested against twelve microbial phytopathogens. From 29 fruit extracts, only 13 were found to be active against three pathogens viz. Botrytis cinerea, Rhizoctonia solani, and Sclerotinia sclerotiorum. Among the nine TLC fractions only one showed presence of a single peak, thus presence of a single compound in the crude extract. Based on lower IC50 value (328 μg/ml) the crude fruit extract collected from Indore region was further characterized. Evaluation against screened phytopathogens indicates its activity. Further, structural characterization of this compound using FTIR and NMR revealed it to be a novel compound i.e. 5-β-Hydroxyl tridecanyl benzoate. Our study paves a way to investigate effective dose of P. murex fruit extracts to utilize in plant protection programs. In future, a better method is required to scale up the process of bioactive molecule (5-β-Hydroxyl tridecayl benzoate) extraction from P. murex or for its chemical synthesis.

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“…GCMS analysis of CLC has shown the presence of some bioactive compounds such as benzimidazole derivatives, eicosane, octacosane and some fatty acids. Octacosane and eicosane are reported to have mosquitocidal and antifungal activities [26][27]. Antimicrobial, antifungal, the anticancerous activity of benzimidazole derivatives are already reported [28][29][30].…”

Section: Discussionmentioning

confidence: 99%

Induction of Autophagy in Human Cervical Cancer Cell Line (Siha) by Chaetomorpha Linum (Muller) Kütz.

Majumder

Paul

Kundu

2018

Int J Pharm Pharm Sci

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Objective: Algae is a rich source of various metabolites. Use of algae in traditional medicine was reported from very early days. The potential anticancerous activity of the algae was also reported by recent researches. In the present study, cell death mechanism induced by green alga Chaetomorpha linum (Muller) Kütz. was studied on human cervical cancer cell line, SiHa (Human papilloma virus-HPV 16+ve).Methods: Cells were treated with chloroform fraction of the Chaetomorpha linum (CLC). DNA fragmentation, cell proliferation, nuclear morphology, localization of autophagosomes and expression of relevant proteins were studied. Cell cycle and mitochondrial membrane potential (MMP) were assessed by fluorescence assisted cell sorting (FACS). Gas chromatography liquid spectrometry (GCMS) analysis of CLC was done to detect the algal compounds. Results:Preliminary experiments confirmed the absence of DNA fragmentation and altered nuclear morphology in the treated cells. In the Acridine Orange stained cells, the presence of autophagosomes was observed by both microscopy and FACS analysis. Decreased MMP was observed in the treated sets. Wound healing assay showed the inhibitory activity of CLC on SiHa cells. Increased expression of autophagic proteins, viz; LC3BII, p62/SQSTM, Beclin-1 and decreased expression of S6 were observed in the treated sets; indicating that autophagic cell death was induced. Preliminary qualitative chemical study and GCMS analysis detected the presence of saponins, glycosides, alkaloids, phenols, steroids and fatty acids in CLC. Conclusion:All these findings clearly suggested that the chloroform fraction of the algae was responsible for induction of apoptosis followed by autophagic cell death in SiHa and can serve as a potential candidate for anticancer drug development in future.

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Extraction and identification of bioactive compounds (eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf

Cited by 130 publications

References 28 publications

Discovery of volatile biomarkers of Parkinson’s disease from sebum

Discovery of volatile biomarkers of Parkinson’s disease from sebum

A novel bioactive molecule 5-β-Hydroxyltridecanyl benzoate identified in Pedalium Murex L. (Gokhru) fruit extracts showed antifungal activity against Sclerotinia sclerotiorum

Induction of Autophagy in Human Cervical Cancer Cell Line (Siha) by Chaetomorpha Linum (Muller) Kütz.

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