Extraction and fractionation of polysaccharides and B-phycoerythrin from the microalga Porphyridium cruentum by membrane technology

Marcati, Alain; Ursu, Alina-Violeta; Laroche, Céline; Soanen, Nastasia; Marchal, Luc; Jubeau, Sébastien; Djelveh, Gholamreza; Michaud, Philippe

doi:10.1016/j.algal.2014.03.006

Cited by 95 publications

(46 citation statements)

References 24 publications

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“…Moreover, this technique is a very efficient separation technology without addition of chemicals and is normally carried out at ambient temperature, which minimizes thermal damage to the product [35]. Marcati et al reported that a first ultrafiltration using a membrane with a MWCO 300 kDa yielded high molecular weight polysaccharides and the resulting 300 kDa permeate was then run through a second MWCO 10 kDa membrane to recover B-PE with a purity ratio of 2.3 [10]. Ruiz-Ruiz et al reported that ultrafiltration with a MWCO 300 kDa membrane could successfully remove most of polyethyleneglycol (PEG) of B-PE solution [9].…”

Section: Fractionation Of Water-soluble Compounds By Ultrafiltrationmentioning

confidence: 99%

“…The purity index of BPE was evaluated spectrophotometrically by the absorbance ratio of A 545 /A 280 . The amounts of B-PE in each step of the process were determined with a previously reported equation system by measures of absorbances at 545, 620 and 650 nm according to Marcati et al [10]. The fluorescence spectrum of purified B-PE was recorded using a fluorescence spectrometer (F-4500, Hitachi, Japan).…”

Section: Analysesmentioning

confidence: 99%

“…R-PE is usually extracted and purified from Porphyra, Grateloupia and Polysiphonia, but most of them contained huge amount of polysaccharides which form gel (carbohydrate) and thereby making the purification task most difficult and tedious [2,24e27]. B-PE is the main phycobiliprotein of Porphyridium species and it contains three subunits (ab) 6 g with molecular weights of 19.5 kDa (a and b subunits) and 29 kDa (g subunit) for a total molecular weight of 263 kDa [10]. This phycoerythrin normally has a double absorption peaks at~545 and 565 nm, and a shoulder peak at~498 nm, and a fluorescence emission maximum at 580 nm [11].…”

Section: Introductionmentioning

confidence: 99%

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One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

Tang

Zhao

Bao

et al. 2016

Protein Expression and Purification

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a b s t r a c t B-phycoerythrin (B-PE) was separated and purified from microalga Porphyridium cruentum using onestep chromatographic method. Phycobiliproteins in P. cruentum was extracted by osmotic shock and initially purified by ultrafiltration. Further purification was carried out with a SOURCE 15Q exchange column and analytical grade B-PE was obtained with a purity ratio (A 545 /A 280 ) of 5.1 and a yield of 68.5%. It showed a double absorption peaks at 545 nm and 565 nm and a shoulder peak at 498 nm, and displayed a fluorescence emission maximum at 580 nm. The analysis by sodium dodecyl sulfate epolyacrylamide gel electrophoresis (SDSePAGE) showed a bulky band between 18 and 20 kDa which could be assigned to subunits a and b and a low intensity band of 27 kDa assigned to g subunit. Our protocol provides attractive alternative to consider for the purification procedure to obtain analytical grade B-PE at commercial level.

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Section: Fractionation Of Water-soluble Compounds By Ultrafiltrationmentioning

confidence: 99%

Section: Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

Tang

Zhao

Bao

et al. 2016

Protein Expression and Purification

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“…The common EPS from these types of microalgae are sulfated and contain different proportions of uronic acids. This is the reason that these EPS are negatively charged and this characteristic contributes to the anionic properties of the biopolymers [52]. For example, all polysaccharide fractions of the EPS from cell-free medium of P. cruentum contain sulfate and are mainly composed of galactose, glucose, xylose, and glucuronic acid [53].…”

Section: Exopolysaccharides (Eps)mentioning

confidence: 99%

Extracellular Metabolites from Industrial Microalgae and Their Biotechnological Potential

2016

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Industrial microalgae, as a big family of promising producers of renewable biomass feedstock, have been commercially exploited for functional food, living feed and feed additives, high-value chemicals in nutraceuticals, cosmeceuticals, and chemical reagents. Recently, microalgae have also been considered as a group that might play an important role in biofuel development and environmental protection. Almost all current products of industrial microalgae are derived from their biomass; however, large amounts of spent cell-free media are available from mass cultivation that is mostly unexploited. In this contribution we discuss that these media, which may contain a remarkable diversity of bioactive substances are worthy to be recovered for further use. Obviously, the extracellular metabolites from industrial microalgae have long been neglected in the development of production methods for valuable metabolites. With the advances in the last ten years, more and more structures and properties from extracellular metabolites have been identified, and the potential utilization over wide fields is attracting attention. Some of these extracellular metabolites can be potentially used as drugs, antioxidants, growth regulators or metal chelators. The purpose of this review is to provide an overview of the known extracellular metabolites from industrial microalgae which might be of commercial interest. The attention mainly focuses on the reports of extracellular bioactive metabolites and their potential application in biotechnology.

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“…Additionally, it also exhibited high efficiencies in the fractionation, purification and concentration of bioactive components such as polysaccharides, peptides, polyphenols, etc. (Jeon et al ., ; Conidi et al ., ; Marcati et al ., ). Furthermore, many researches mainly paid attention to the effect of membrane processing on the removal of yeast, and bacteria and their spores for food safety (Guerra et al ., ; Lipnizki, ).…”

Section: Introductionmentioning

confidence: 97%

Removal of imidacloprid and acetamiprid from tea infusions by microfiltration membrane

Zhang

Huang

Bing

et al. 2015

Int J of Food Sci Tech

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Removal of imidacloprid and acetamiprid in tea infusions by microfiltration membrane using dead-end model was investigated in the present study. The results showed that microfiltration significantly promoted the removal of both pesticides (P < 0.05) in tea infusions. Furthermore, the extent of removal was strongly influenced by the pore size of membrane, operational pressure and the concentrations of tea infusions. The initial concentration of imidacloprid and acetamiprid showed no significant effect on their removal rates. The maximum removal rates were 79.7% for imidacloprid and 81.9% acetamiprid. The changes in major chemical components of tea infusions after microfiltration were evaluated. The results indicated that microfiltration caused no considerable changes in total polyphenols and total free amino acids, and small but statistically significant losses (6.3-18.0%) of eight catechins and three methylxanthines when filtration volume reached to 200 mL. The present study validated the application of microfiltration as a potentially feasible and promising method for the removal of imidacloprid and acetamiprid residues from tea infusions.Elimination of pesticides by microfiltration Y. Zhang et al. 1398 Elimination of pesticides by microfiltration Y. Zhang et al.

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Extraction and fractionation of polysaccharides and B-phycoerythrin from the microalga Porphyridium cruentum by membrane technology

Cited by 95 publications

References 24 publications

One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

Extracellular Metabolites from Industrial Microalgae and Their Biotechnological Potential

Removal of imidacloprid and acetamiprid from tea infusions by microfiltration membrane

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