Extraction and determination of oxybutynin in human bladder samples by reversed-phase high-performance liquid chromatography

Massoud, Renato; Federici, Giorgio; Casciani, Stefania; Stasi, Savino M. Di; Fucci, Giorgio; Giannantoni, Antonella; Cortese, Claudio

doi:10.1016/s0378-4347(99)00326-6

Cited by 15 publications

(5 citation statements)

References 8 publications

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“…1) is a chiral drug administrated as a racemic mixture for the treatment of urinary incontinence [1,2]. Anticholinergic agents, such as OXY, inhibit the binding of acetylcholine to the cholinergic receptor and suppress involuntary bladder contractions [3]. After oral administration, OXY is extensively biotransformated by cytochrome P450 enzymes to N-desethyloxybutynin (DEO, Fig.…”

Section: Oxybutyninmentioning

confidence: 99%

“…The factor that demonstrated the lowest influence in the OXY and DEO extraction was the concentration of the acid in the acceptor phase, so trifluoracetic acid 0.1 mol L −1 was selected for subsequent experiments and this factor was not evaluated in the CCD. The donor phase pH exerted great influence in the extraction of the analytes, but it was not evaluated in the CCD because OXY has stability problems in pH values higher than 8.0 [3]. So, the CCD study was outlined with four factors: extraction time, sample agitation and salt and methanol addition to donor phase.…”

Section: Optimization Of the Lpme Proceduresmentioning

confidence: 99%

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Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study☆

Fonseca

Freitas

Pinto

et al. 2008

Journal of Chromatography B

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Section: Oxybutyninmentioning

confidence: 99%

Section: Optimization Of the Lpme Proceduresmentioning

confidence: 99%

Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study☆

Fonseca

Freitas

Pinto

et al. 2008

Journal of Chromatography B

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“…Massoud et al found that high-performance liquid chromatography (HPLC), equipped with a diode-array spectrophotometric detector, an electrochemical detector and reversed-phase column, was a useful method to determine tissue concentrations of oxybutynin [39]. The procedure was then applied for tissue extraction and the determination of other agents ( Table 1).…”

Section: Recent Developments In Physical Approachesmentioning

confidence: 99%

New Frontiers in Intravesical Therapies and Drug Delivery

et al. 2006

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e u r o p e a n u r o l o g y 5 0 ( 2 0 0 6 ) 1 1 8 3 -1 1 9 3 a v a i l a b l e a t w w w . s c i e n c e d i r e c t . c o m j o u r n a l h o m e p a g e : w w w . e u r o p e a n u r o l o g y . c o m Article info AbstractObjectives: The intravesical route permits site-specific delivery of drugs with a reduced side-effect profile as compared to oral delivery systems, either by avoiding first-pass metabolism or by obtaining a local effect. We investigated mechanisms related to urothelium permeability and new physical and chemical developments in intravesical drug delivery that potentially permit successful treatment of several bladder dysfunction. Methods: A literature review. Results: Pharmacologic agents increasing urothelial permeability and useful for clinical purposes have been described, such as dimethylsulfoxide, protamine sulphate, chitosan, and nystatin. Among physical approaches, electromotive drug administration appears to be more effective than intravesical passive diffusion in delivering drugs through the urothelium into deeper layers of the bladder. Experimental and clinical reports demonstrated that electric current significantly increases the transport of local anaesthetics, mytomicin C, oxybutynin, resiniferatoxin, epinephrine, and dexamethasone. Among new chemical approaches, cell-penetrating peptides posses the ability to translocate macromolecular drugs across membranes of urothelial cells. The therapeutic benefits of sustained delivery afforded by thermosensitive hydrogel, which forms a depot for hydrophilic and hydrophobic drugs, have been demonstrated by delivering anti-inflammatory drugs. Liposomes improve the aqueous solubility of several hydrophobic drugs such as taxol, amphotericin, and capsaicin. Conclusions: Electromotive drug administration, new in situ delivery systems, and bioadhesive liposomes may make it possible to extend intravesical therapy and drug administration to many bladder diseases. Research to expand knowledge of the chemical and physical properties of the bladder and processes regulating drug transport across biologic membranes is needed to make this a reality.

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“…In various literature, High Performance Liquid Chromatographic (HPLC), [3][4][5][6][7][8][9] High performance frontal analysis, [10,11] GC-MS, [12,13] and LC-MS [14,15] methods are reported for the estimation of OXB HCL in various matrix systems.…”

Section: Introductionmentioning

confidence: 99%

Development of a Validated Stability Indicating Lc Method for Quantitative Determination of an Antispasmodic Drug and Its Related Substances

Pai¹,

Dubhashi²

2010

Journal of Liquid Chromatography & Related Technologies

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& Present article describes development and validation of stability indicating liquid chromatographic method for quantitative determination of a-cyclohexyl-a-hydroxy-benzeneacetic acid-4-(diethylamino)-2-butynyl ester hydrochloride a potential antispasmodic and anticholinergic drug and its four impurities (related substances) using a Zorbax SB-Cyano column and mobile phase consisting of aqueous buffer (0.20% triethylamine) and acetonitrile in the ratio 49:51 and with a pH 6.3. The developed LC method was validated with respect to linearity, specificity, accuracy, precision, limit of detection, and limit of quantitation. For the quantitative determination of the impurities, a relative response factor approach was applied with respect to the drug substance. The developed LC method for the related substances and assay determination of drug can be used to evaluate quality of regular production samples. It can also be used to test the stability samples of the drug substance.

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Extraction and determination of oxybutynin in human bladder samples by reversed-phase high-performance liquid chromatography

Cited by 15 publications

References 8 publications

Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study☆

Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study☆

New Frontiers in Intravesical Therapies and Drug Delivery

Development of a Validated Stability Indicating Lc Method for Quantitative Determination of an Antispasmodic Drug and Its Related Substances

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