Extraction and characterization of the starch present in the avocado seed (Persea americana mill) for future applications

Martins, S.H.F.; Pontes, Karen Valverde; Fialho, Rosana Lopes; Fakhouri, Farayde Matta

doi:10.1016/j.jafr.2022.100303

Cited by 13 publications

(11 citation statements)

References 63 publications

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“…The content of extractives in the seeds was 25.92 ± 1.31 and lignin 3.23 ± 0.63; these values are similar to those reported by other research groups (see Table 1). [73,74]. So, the method used for the extraction of starch in the present work obtained a higher result and can be used for the next sequential steps of hydrolysis of the starch polymers to sugar units and fermentation for bioethanol production.…”

Section: Characterization Of Avocado Seedsmentioning

confidence: 84%

“…These two residues are rich in carbohydrates such as cellulose, hemicellulose, and starch and have a high potential for the production of value-added materials [18]. Speci cally, the chemical composition of seed on Hass and Fuerte varieties is reported 2.4 and 2.5% protein, 3.5 and 2.2% sugar, 2.5 and 3.2% neutral lipids, 12 and 13% glycolipids, 7.4 and 10.9% phospholipids, 0.8 and 1.0% fat, respectively [19][20][21]. Starch represents nearly 60% of the seed (dry matter basis), resulting in large amounts of potentially fermentable sugars [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

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Pilot-scale bioethanol production from the starch of avocado seeds using a combination of dilute acid-based hydrolysis and alcoholic fermentation by Saccharomyces cerevisiae

Caballero-Sanchez

Lázaro-Mixteco

Vargas‐Tah

et al. 2022

Preprint

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Background A processing methodology of raw starch extraction from avocado seeds (ASs) and a sequential hydrolysis and fermentation bioprocess in just a few steps was successfully obtained for the bioethanol production by a single yeast Saccharomyces cerevisiae strain and this research was also to investigate the optimum conditions for the pretreatment of biomass and technical procedures for the production of bioethanol. It successfully resulted in high yields and productivity of all the experiments from the laboratory scale and the pilot plant. Ethanol yields from pretreated starch are comparable with those in commercial industries that use molasses and hydrolyzed starch as raw materials. Results Before the pilot-scale bioethanol production, studies of starch extraction and dilute sulfuric acid-based pretreatment was carefully conducted. The amount of starch extracted from dry and fresh avocado seed was 16.85 g ± 0.34 g and 29.79 ± 3.18 g of dry starch, representing a yield of ∼17 % and 30 %, respectively. After a dilute sulfuric acid pretreatment of starch, the released reducing sugars (RRS) were obtained and the hydrolysate slurries containing glucose (109.79 ± 1.14 g/L), xylose (0.99 ± 0.06 g/L), and arabinose (0.38 ± 0.01 g/L). The efficiency of total sugar conversion was 73.40 %, with a productivity of 9.26 g/L/h. The ethanol fermentation in a 125 mL flask fermenter showed that Saccharomyces cerevisiae (Fali, active dry yeast) produced the maximum ethanol concentration, pmax at 49.05 g/L (6.22% v/v) with a yield coefficient, Yp/s of 0.44 gEthanol/gGlucose, a productivity or production rate, rp at 2.01 g/L/h and an efficiency, Ef of 85.37%. The pilot scale experiments of the ethanol fermentation using the 40-L fermenter were also successfully achieved with essentially good results. The values of pmax, Yp/s, rp, and Ef of the 40-L scale were at 50.94 g/L (6.46% v/v), 0.45 gEthanol/gGlucose, 2.11 g/L/h, and 88.74%, respectively. Because of using raw starch, major by-products, i.e., acetic acid in the two scales were very low, in ranges of 0.88–2.45 g/L, and lactic acid was not produced, which are less than those values in the industries. Conclusion The sequential hydrolysis and fermentation process of two scales for ethanol production using the combination of hydrolysis by utilizing dilute sulfuric acid-based pretreatment and fermentation by a single yeast Saccharomyces cerevisiae strain is practicable and feasible for realistic and effective scale-up strategies of bioethanol production from the starch of avocado seeds.

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Section: Characterization Of Avocado Seedsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Pilot-scale bioethanol production from the starch of avocado seeds using a combination of dilute acid-based hydrolysis and alcoholic fermentation by Saccharomyces cerevisiae

Caballero-Sanchez

Lázaro-Mixteco

Vargas‐Tah

et al. 2022

Preprint

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“…A study with Hass avocado seed starch found values for final viscosity of 448.79 RVU, a setback of 240.04 RVU and a pasting temperature of 88.5 °C (Martins et al, 2022) Pasting properties are important results on aerogel production and can influence their properties as water absorption and resistance to compression since starch gelatinisation and retrogradation influence the porous formation and, consequently, the aerogel properties. The rearrangement and interaction of amylose and amylopectin provided a three-dimensional network formation and can influence the water absorption capacity of the aerogel.…”

Section: Pasting Propertiesmentioning

confidence: 99%

Extraction and characterisation of avocado seed starch (Persea americana Miller) and application in aerogels

Frasson,

Nunes,

Biduski

et al. 2023

Int J of Food Sci Tech

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SummaryAvocado seed (Persea americana Miller) is a by‐product of fruit processing and is considered a potential alternative source of starch. The use of starches from unconventional sources can be an alternative to replace modified starches as a functional ingredient in food systems. This study aimed to extract and characterize starch from avocado seed varieties Fortuna and Hass and produce aerogels. Starches were characterized by physical, chemical, thermal, and morphological properties. The aerogels were produced by physical crosslinking and evaluated for water absorption capacity and physical properties. The starch extraction yield was 12%, and the granules had oval and spherical morphology. Amylose content ranged from 41.95% to 49.46%, and relative crystallinity from 22% to 25% for Hass and Fortuna, respectively. The Fortuna starch showed higher pasting temperature, peak viscosity, peak time, and final viscosity values. Aerogels had excellent structural characteristics and had not deformed after 24 hours of hydration; the Fortuna starch aerogels showed higher water absorption and mechanical resistance. The properties of avocado seed starch indicate a vast potential for application in food industries and for developing biodegradable packaging with excellent physical properties and excellent water retention capacity.

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“…e wet, milled, fine avocado seed was mixed with 2 litres of distilled water. en sodium metabisulfite was added at a concentration of 0.03% and the mixture was left for 24 h [8] to avoid enzymatic oxidation, mainly by peroxidases and phenolates, and to prevent the browning reaction. e starch slurry was filtered using a muslin cloth, and the filtrate was left for an hour to settle the starch.…”

Section: Starch Extractionmentioning

confidence: 99%

Extraction and Characterisation of Avocado Seed Starch, and Its Blend with Enset Cellulosic

Alemu

Balakrishnan

Debtera

2022

Advances in Materials Science and Engineering

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Avocado seed starch was extracted to prepare a biodegradable plastic film with enset cellulosic reinforcement and glycerol plasticiser. The characterisations were made in detail. Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimeter (DSC), scanning electron microscopy (SEM), X-ray diffraction (XRD), rheometer, and Rapid Visco Analyser (RVA) techniques were employed to characterise the physicochemical properties of avocado seed starch, enset cellulosic, and the blend. Starch and enset cellulosic extraction yields are 18.3 ± 0.02% and 67.5 ± 0.08%, respectively. As a result, avocado seed starch and enset cellulosic blend can be used as alternative raw materials to develop biodegradable plastics.

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Extraction and characterization of the starch present in the avocado seed (Persea americana mill) for future applications

Cited by 13 publications

References 63 publications

Pilot-scale bioethanol production from the starch of avocado seeds using a combination of dilute acid-based hydrolysis and alcoholic fermentation by Saccharomyces cerevisiae

Pilot-scale bioethanol production from the starch of avocado seeds using a combination of dilute acid-based hydrolysis and alcoholic fermentation by Saccharomyces cerevisiae

Extraction and characterisation of avocado seed starch (Persea americana Miller) and application in aerogels

Extraction and Characterisation of Avocado Seed Starch, and Its Blend with Enset Cellulosic

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