Extraction and characterization of phycocyanin from Spirulina platensis and evaluation of its anticancer, antidiabetic and antiinflammatory effect

Prabakaran, Gopal; Sampathkumar, P.; Kavisri, M.; Moovendhan, Meivelu

doi:10.1016/j.ijbiomac.2020.03.009

Cited by 116 publications

(65 citation statements)

References 36 publications

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“…Oscillatoriaceae 13 that occurs naturally in alkaline lakes. 14 As a functional resource, S platensis contains various active ingredients, such as vitamins, minerals, phenolic acids, beta-carotene, proteins and tocopherols. It also exhibits high levels of antioxidant and anti-inflammatory activities, 15 including essential amino acids.…”

Section: Spirulina Platensis (S Platensis) Is Part Of the Family Ofmentioning

confidence: 99%

Spirulina platensis alleviates chronic inflammation with modulation of gut microbiota and intestinal permeability in rats fed a high‐fat diet

Wang

Chen

et al. 2020

J Cellular Molecular Medi

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Recent research suggested that taking a high‐fat diet (HFD) may lead to a gut microbiota imbalance and colon tissue damage. This would lead to increased intestinal permeability and consequent constant circulation of low‐grade inflammatory cytokines. Spirulina platensis can protect against HFD‐induced metabolic inflammation and can stimulate the growth of beneficial bacteria in in vitro stool cultures. However, it is unknown whether this beneficial effect acts on intestinal tissues. In this study, rats were fed a high‐fat diet fed with 3% S platensis for 14 weeks. We analysed endotoxin, the composition of the microbiota, inflammation and gut permeability. We found that S platensis decreased the bodyweight and visceral fat pads weight of the HFD‐fed rats. In addition, it lowered the levels of lipopolysaccharide and pro‐inflammatory cytokines in serum. Our results showed that S platensis could largely reduce the relative amount of Proteobacteria and the Firmicutes/Bacteroidetes ratio in faecal samples from HFD‐fed rats. S platensis significantly reduced intestinal inflammation, as shown by decreased expression of myeloid differentiation factor 88 (MyD88), toll‐like receptor 4 (TLR4), NF‐κB (p65) and inflammatory cytokines. S platensis also ameliorated the increased permeability and decreased expression of tight junction proteins in the intestinal mucosa, such as ZO‐1, Occludin and Claudin‐1. Therefore, in HFD‐induced gut dysbiosis rats, S platensis benefits health by inhibiting chronic inflammation and gut dysbiosis, and modulating gut permeability.

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Section: Spirulina Platensis (S Platensis) Is Part Of the Family Ofmentioning

confidence: 99%

Spirulina platensis alleviates chronic inflammation with modulation of gut microbiota and intestinal permeability in rats fed a high‐fat diet

Wang

Chen

et al. 2020

J Cellular Molecular Medi

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“…has also been reported (Table 1) [41,43]. Cphycocyanin showed inhibitory activity in liver cancer cell lines (HepG2) [57], human leukemia cells (K562) [58] and against lung cancer cell lines (A549 and NSCLC) [39,40,41]. In another study, the phycocyanin from Limnothrix sp.…”

Section: Compounds With Anti-cancer Propertiesmentioning

confidence: 77%

“…Isolation of C-phycocyanin from Spirulina platensis was accomplished using buffer ammonium sulfate solution (i.e. salting-out technique) as well as by using supercritical fluid extraction using ethanol as a modifier which resulted in higher yield compared to conventional solvent extraction [41,57]. In the studies, impurities were removed using chitosan and activated charcoal however further purified the compound by ion-exchange chromatography was also carried out [43].…”

Section: Compounds With Anti-cancer Propertiesmentioning

confidence: 99%

Isolation of Industrial Important Bioactive Compounds From Microalgae: A Review

Balasubramaniam¹,

Gunasegavan²,

Mustar³

et al. 2020

Preprint

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Microalgae are known as a rich source of bioactive compounds which exhibit different biological activities. Increased demand for sustainable biomass for production of important bioactive components with various potential especially therapeutic applications has resulted in noticeable interest in algae. Utilization of microalgae in multiple scopes has been growing in various industries ranging from harnessing renewable energy to exploitation of high-value products. The focuses of this review are on the isolation of bioactive compounds from microalgae regarding different metabolites which are currently used and new possible applications of the compounds in industries and future prospects. Moreover, this work discusses the advantage, potential new beneficial strains, applications, limitations, research gaps and future prospect of microalgae in industry.

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“…Water-soluble C-phycocyanin can be easily extracted from spirulina. Ultrasonication method was chosen for its efficiency (Furuki et al, 2003;Prabakaran et al, 2020). 1.5 g of cryogenised beads was weighted in eppendorf-safe lock tubes (2.0 mL) and stored until melting at 20°C (20 min).…”

Section: Spectrophotometric Determination Of Phycocyanin-c Contentmentioning

confidence: 99%

“…The phycobilisomes absorb light in the wavelength range of 500-650 nm and transfer energy to the chlorophyll for photosynthesis (Liu et al, 2005). In spirulina, phycocyanin-C represents the major phycobiliproteins that is already used in cosmetic and approved as food colorant (Prabakaran et al, 2020). In current extensive cultivation systems for spirulina biomass production, the concentration of phycocyanin-C varies from 27 to 96 mg g −1 of dry biomass (Bermudez et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Influence of pressurised cryogenic nitrogen technology on Arthrospira platensis (spirulina) preservation during storage

Maujean

Desobry

Gillet

et al. 2020

Int J of Food Sci Tech

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In this study, preservation of spirulina using the new pressurised cryogenic nitrogen technology (PCN) was compared to classical methods used in laboratories and industry. Spirulina morphology was better preserved by PCN compared to unpressurised cryogeny and classical freezing at −20°C that led to cells fragmentation. A 25% loss of Phycocyanin-C content against 60% were measured after 98 days storage for 6-Bar PCN process and frozen samples, respectively. The Total AntiOxidant Power (PAOT Liquid Technology ® ) was used for determination of total antioxidant and oxidant power of spirulina extracts. PAOT value of PCN samples was 50% higher than the frozen sample. From ABTS measurements on PCN spirulina fractions sonicated or not, it was suggested that pressurisation at 6 bars allowed a better preservation of free antioxidants (outside the cells) due to replacement of oxygen by nitrogen in the frozen beads. After dehydration, phycocyanin-C content variation during storage at 20°C and 33% RH showed higher loss for freeze-dried spirulina treated at 0 compared to 6 Bars.

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Extraction and characterization of phycocyanin from Spirulina platensis and evaluation of its anticancer, antidiabetic and antiinflammatory effect

Cited by 116 publications

References 36 publications

Spirulina platensis alleviates chronic inflammation with modulation of gut microbiota and intestinal permeability in rats fed a high‐fat diet

Spirulina platensis alleviates chronic inflammation with modulation of gut microbiota and intestinal permeability in rats fed a high‐fat diet

Isolation of Industrial Important Bioactive Compounds From Microalgae: A Review

Influence of pressurised cryogenic nitrogen technology on Arthrospira platensis (spirulina) preservation during storage

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