Extraction and antioxidant activity of total triterpenoids in the mycelium of a medicinal fungus, Sanghuangporus sanghuang

Cai, Chengshan; Ma, Jie-xin; Han, Chunrui; Jin, Yi; Zhao, Guozhu; He, Xiangwei

doi:10.1038/s41598-019-43886-0

Cited by 69 publications

(88 citation statements)

References 17 publications

(17 reference statements)

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“…A study by Jin et al, 2019, reported that genistein reduced inflammation [31]. NF-κβ act as signals of inflammation and some research suggests that this pathway can enable high lipid, TNF-α, endotoxin, and TLR4 associated with inflammation [32].…”

Section: Genistein As Antioxidantmentioning

confidence: 99%

Antioxidant Activities of Genistein: A Review

Burhan¹,

Oktavia²,

Fauziah³

2020

IJPSM

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Section: Genistein As Antioxidantmentioning

confidence: 99%

Antioxidant Activities of Genistein: A Review

Burhan¹,

Oktavia²,

Fauziah³

2020

IJPSM

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“…Members of the family Cucurbitaceae have proved to be promising antioxidants as reported by earlier workers 2,3,17,22 . In addition, various methods have been employed for the analysis of cucurbitacins using TLC, FTIR, HPLC, LC-MS 17,23 . Hence, the goals of the current study were to optimize extraction of CUI, CUB and CUI + B using HPLC and RSM as a suitable statistical tool.…”

mentioning

confidence: 99%

Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Patel

Attar

Sakate

et al. 2020

Sci Rep

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Diplocyclos palmatus (L.) C. Jeffrey is an important medicinal plant used in several reproductive medicines. It serves as a wide source of tetracyclic triterpens called cucurbitacins. Response surface methodology (RSM) with Box-Behnken design (BBD) was studied to optimize the production of cucurbitacins. RSM put forth the ideal conditions such as 1:30 SS ratio (g/mL), 80 rpm (mixing extraction speed), 150 µm mean particle size, 30 min extraction time and 50 °C using chloroform in continuous shaking extraction (CSE) and showed the highest cucurbitacin I (CUI) content (2.345 ± 0.1686 mg/g DW). Similarly, the highest yield of cucurbitacin B (CUB) (1.584 ± 0.15 mg/g DW) was recorded at ideal conditions (1:40 g/mL SS ratio and 60 min time and others similar to CUI). Among the tested extraction methods, the highest CUI, CUB, and CUI + B yield (1.437 ± 0.03, 0.782 ± 0.10, 2.17 ± 0.35 mg/g DW, respectively) as well as promising DPPH radical scavenging activity (25.06 ± 0.1 µgAAE/g DW) were recorded from the SBAE (steam bath assisted extraction). In addition, MAE and UAE revealed the highest inhibition of α-amylase (68.68%) and α-glucosidase (56.27%) enzymes, respectively. Fruit extracts showed potent anticancer activity against breast (MCF-7) and colon (HT-29) cancer cell lines (LC 50 -44.27 and 46.88 µg/mL, respectively). Our study proved that SS ratio, particle size and temperature were the most positively influencing variables and served to be the most efficient for the highest recovery of CUI and CUB. Based on the present study, the fruits of D. palmatus were revealed as a potent antioxidant, anti-diabetic and anticancer bio-resource that could be explored further to develop novel drug to manage diabetes, cancer and oxidative stress related disorders.The plants from the family Cucurbitaceae are commonly called as melons, squashes, and gourds which are traditionally used in the human diet. It comprises 122 genera and 940 species of which 31 genera and 94 species are found in India 1 . Diplocyclos palmatus (L.) C. Jeffrey is a slender-stemmed tendril climber commonly called as Shivlingi. Traditionally, this plant has been used in the folk medicine and possesses several activities such as gynaecological, anti-asthmatic, anti-convulsant, anti-venom, anti-inflammatory, androgenic and antioxidant 2-4 . The family Cucurbitaceae has been recognized as a rich source of bitter compounds, called cucurbitacins 5 . The cucurbitacins are highly unsaturated triterpenes containing many keto-, hydroxy-and acetoxy-groups. They are the major active components in traditional medicine, herbal remedies and Pharmacopoeia to treat various health issues such as hepatoprotective, anti-fertility, diabroticites cardiovascular and anti-inflammatory activities 6 . In a recent study, cucurbitacin I (CUI) has been identified as a strong inhibitor of the JAK2/STAT3 signaling pathway (a common oncogenic signaling pathway), which is constitutively activated in many types of cancer; hence, it is considered as a milestone in cancer therapy 7 . Similarly...

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“…Penelitian terkait senyawa golongan saponin dan triterpenoid pada madu dengan aktivitas antioksidan masih belum ada. Namun pada sampel uji bukan madu, senyawa golongan saponin dan triterpenoid dilaporkan memiliki aktivitas antioksidan (Cai et al, 2019;Nafiu and Ashafa, 2017). Salah satu senyawa saponin dilaporkan memiliki aktivitas penangkap radikal bebas dengan mekanisme yang sama seperti senyawa golongan flavonoid (Yoshiki and Okubo, 1995).…”

Section: Pendahuluanunclassified

Aktivitas Antioksidan Kombinasi Fraksi Metanol Virgin Coconut Oil dan Madu Kele Bali dengan Metode DPPH (2,2-diphenyl-1-picrylhidrazyl)

Leliqia

Harta

Saputra

et al. 2020

J. Pharm. Sci. Clin. Res.

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Virgin coconut oil (VCO) dan madu telah banyak digunakan sebagai bahan baku sediaan topikal untuk perawatan kulit. Salah satu khasiat yang telah terbukti adalah aktivitas antioksidannya. Namun efek VCO dan madu sebagai antioksidan umumnya masih dibuktikan dalam bentuk bahan tunggal tidak dalam bentuk kombinasi keduanya. Pengujian aktivitas antioksidan dengan metode DPPH memerlukan sampel uji yang bersifat polar. Fraksi metanol dari VCO dan madu telah diketahui bersifat polar. Tujuan penelitian ini adalah menentukan kombinasi fraksi metanol VCO dan madu kele Bali yang memberikan aktivitas antioksidan paling kuat. VCO dan madu kele Bali diperoleh dari Desa Aan, Bali. Skrining fitokimia fraksi metanol VCO dan madu kele Bali ditentukan ditentukan dengan metode yang sesuai. Aktivitas antioksidan (IC50) fraksi metanol VCO, madu kele Bali, dan kombinasi keduanya ditentukan dengan metode DPPH. Terdapat 5 kombinasi kedua bahan tersebut yang diuji. Data dianalisis menggunakan ANOVA oneway-LSD dengan taraf kepercayaan 95%. Fraksi metanol VCO mengandung flavonoid dan tanin, sedangkan madu kele Bali mengandung saponin, fenol, flavonoid dan steroid/triterpenoid. Fraksi metanol VCO dan madu kele Bali memiliki aktivitas antioksidan dengan nilai IC50 berturut-turut sebesar 7,20±0,23 mg/mL dan 14,56±0,25 mg/mL. Perbandingan jumlah kombinasi fraksi metanol VCO dan madu kele Bali berpengaruh secara bermakna terhadap aktivitas antioksidannya (p<0,05). Kombinasi 0,0063 g fraksi metanol VCO dan 1 g madu kele Bali menunjukkan aktivitas antioksidan paling kuat (IC50 8,50±0,17 mg/mL). Fraksi metanol VCO dan madu kele Bali serta kombinasinya tergolong antioksidan lemah, namun terjadi peningkatan aktivitas antioksidan yang bermakna pada kombinasi ini dibandingkan madu kele Bali tunggal (p<0,05).

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Extraction and antioxidant activity of total triterpenoids in the mycelium of a medicinal fungus, Sanghuangporus sanghuang

Cited by 69 publications

References 17 publications

Antioxidant Activities of Genistein: A Review

Antioxidant Activities of Genistein: A Review

Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Aktivitas Antioksidan Kombinasi Fraksi Metanol Virgin Coconut Oil dan Madu Kele Bali dengan Metode DPPH (2,2-diphenyl-1-picrylhidrazyl)

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