Extraction & Characterization of Chitin & Chitosan from Bionetria CBNR BKRR, Synthesis of their Bionanocomposites and Study of their Application

Abstract

Chitin and chitosan hold a great economic value due to their versatile biological activities and chemical applications, mainly in medical and pharmaceutical. Bionectria CBNR KRRR, isolated from the marine soils of Pichavaram, Tamil Nadu was used for the economic production of Chitin and Chitosan using three different media-Sabouraud sucrose broth, Hesseltine and Anderson medium, Andrade et al medium. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy. The highest growth rate was with Andarde et al medium with a mycelial dry weight of 15.21g/L. The best yields of the polysaccharides (mg per gram of dry mycelia biomass) are obtained with Sabouraud sucrose broth for chitosan (204 mg/g or 20%) and for chitin (2189.78 mg/g or 218%).From the SEM image, Chitin AgNP's exhibited smaller powder particle size. Ag/Chitosan BNCs showed show strong needle shaped structures. The EDS spectrum of Bionectria CBNR KRRR CS AgNP composite shows the peaks of C K, O K, Cl K and Ag L. The atomic ratio of was found to be 85:13:0.34:1.45 wt %. The EDS spectrum of Bionectria CBNR KRRR CS AgNP composite shows the peaks of C O K, and Ag L. The atomic ratio of was found to be 15:17:67 wt %. The antibacterial activity of Chitin and Chitosan solution was found to be less than the Bionanocomposites indicating that the presence of the silver ion thereby increases the antibacterial strength of the polysaccharides. Chitin AgNP showed 94.5% dye inhibition in 72 hours and Chitosan AgNP showed 97.5% inhibition.

Fabrication and Characterization of Electrospun Waste Polyethylene Terephthalate Blended with Chitosan: A Potential Single-Use Material

“…The band at 1068 cm −1 confirms the C-O functional group [ 23 , 24 ]. The functional groups of the electrospun commercial CS/TFA-DCM nanofiber are similar to the bands 3429 cm −1 , 1651 cm −1 , and 1417 cm −1 of the commercial chitosan used in the study reported by Kumirska et al (2010); thus, the chitosan nanofibers were structurally similar to chitosan [ 25 , 26 ], not chitin.…”

Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum

“…Significant shifts of peaks in the spectrum of CNPs from peaks in the spectrum of the chitosan standard indicate a significant role of functional groups in the CNPs biosynthesis. The presence of bands at 3442 cm −1 in the chitosan standard sample indicates strong dimeric OH stretch 56 . Moreover, on the formation of nanoparticles, the hydroxyl (OH) stretching peak at wavelength 3442.09 cm −1 found in the spectrum of chitosan standard shifted to 3326.66 cm −1 which indicates the stretching vibrations of OH groups (Fig.…”

“…Krishnaveni and Ragunathan 56 , 58 reported that the bands at 1655 cm −1 , and those at 1641, 1642 cm −1 indicate the presence of the amide I region. Further, the band at 1654.98 cm −1 (around 1655 cm −1 ) indicates the stretching vibration of type I amide.…”

Multifunctional Biodegradable Microrobot with Programmable Morphology for Biomedical Applications

“…CMS was fabricated from a chitosan-based porous sheet via laser micromachining (Figure a). Chitosan is known to have high energy absorbance in the UV wavelength region (300–400 nm) . Hence, the femtosecond-pulsed UV laser with a 343 nm wavelength and 190 fs pulse duration was used to micromachine the chitosan-based porous sheet.…”

“…Chitosan is known to have high energy absorbance in the UV wavelength region (300−400 nm). 28 Hence, the femtosecond-pulsed UV laser with a 343 nm wavelength and 190 fs pulse duration was used to micromachine the chitosan-based porous sheet. In order to minimize damage to the sheet by the laser, the laser power was optimized by testing several samples.…”