Extracting relevant information for cancer diagnosis from dynamic full field OCT through image processing and learning

Apelian, Clément; Gastaud, Clément; Boccara, A. C.

doi:10.1117/12.2254824

Cited by 7 publications

(9 citation statements)

References 6 publications

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“…The inability to appreciate cellular details in OCT images has been overcome to a large extent with the development of FF-OCT and dynamic (D)-FFOCT techniques that can generate high-resolution, 3-dimensional real-time tomographic images from unprocessed and unlabeled tissue. [30][31][32][33] Similar to conventional OCT systems, the FF-OCT imaging technique is based on the principle of white-light interference microscopy. A simple tungsten halogen lamp is used as a spatially incoherent source to illuminate the entire field of a water-immersion microscope objective.…”

Section: Optical Coherence Tomographymentioning

confidence: 99%

Ex Vivo Microscopy: A Promising Next-Generation Digital Microscopy Tool for Surgical Pathology Practice

Krishnamurthy

Brown

Iftimia

et al. 2019

Archives of Pathology &Amp; Laboratory Medicine

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Context.— The rapid evolution of optical imaging modalities in recent years has opened the opportunity for ex vivo tissue imaging, which has significant implications for surgical pathology practice. These modalities have promising potential to be used as next-generation digital microscopy tools for examination of fresh tissue, with or without labeling with contrast agents. Objective.— To review the literature regarding various types of ex vivo optical imaging platforms that can generate digital images for tissue recognition with potential for utilization in anatomic pathology clinical practices. Data Sources.— Literature relevant to ex vivo tissue imaging obtained from the PubMed database. Conclusions.— Ex vivo imaging of tissues can be performed by using various types of optical imaging techniques. These next-generation digital microscopy tools have a promising potential for utilization in surgical pathology practice.

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Section: Optical Coherence Tomographymentioning

confidence: 99%

Ex Vivo Microscopy: A Promising Next-Generation Digital Microscopy Tool for Surgical Pathology Practice

Krishnamurthy

Brown

Iftimia

et al. 2019

Archives of Pathology &Amp; Laboratory Medicine

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“…Several other techniques to analyze interferometric signal fluctuations are discussed in a previous publication. 17…”

Section: Imaging Setupsmentioning

confidence: 99%

“…[4][5][6][7][8], the Fourier transform of the temporal fluctuations of each single pixel is calculated and divided into three normalized frequency bands. 17 The red channel corresponds to the low frequency spectrum intensity (fluctuations between 0.1 and 0.5 Hz), green is associated with intermediate dynamics (fluctuations between 0.5 and 5 Hz) and blue with the fastest fluctuations (above 5 Hz). All channels are normalized to the maximal value of each channel of each image.…”

Section: Image Processingmentioning

confidence: 99%

“…The faster the fluctuations are, the faster the signal decorrelates, so that calculating frequency bands gives similar results in dynamic FFOCT to computing a decorrelation time or a fractal dimension. 17,18 Here, we used frequency bands as they are much faster to compute for the large data sets involved (each plane corresponds to 4 GB of data). Identical color scale was used for color-coded images in Figures 4 through 8.…”

Section: Image Processingmentioning

confidence: 99%

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Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography

Thouvenin

Boccara

Fink

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…Unlike fluorescence imaging technologies, OCT employs low-coherence interferometry to detect back-scattered signals and provides reconstructed depth-resolved images at micron-level spatial resolutions. In the field of oncology, both standard and functional OCT have been used to analyse pathological features of tumors and evaluate their margins ex vivo in tissues (31–35). OCT also facilitates in vivo studies of murine models harbouring implanted tumors and detects their margins, microenvironment, and vascular dynamics (36,37).…”

Section: Introductionmentioning

confidence: 99%

Optical Coherence Tomography Detects Necrotic Regions and Volumetrically Quantifies Multicellular Tumor Spheroids

et al. 2017

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Three-dimensional (3D) tumor spheroid models have gained increased recognition as important tools in cancer research and anti-cancer drug development. However, currently available imaging approaches employed in high-throughput screening drug discovery platforms e.g. bright field, phase contrast, and fluorescence microscopies, are unable to resolve 3D structures deep inside (>50 µm) tumor spheroids. In this study, we established a label-free, non-invasive optical coherence tomography (OCT) imaging platform to characterize 3D morphological and physiological information of multicellular tumor spheroids (MCTS) growing from ~250 µm up to ~600 µm in height over 21 days. In particular, tumor spheroids of two cell lines glioblastoma (U-87 MG) and colorectal carcinoma (HCT 116) exhibited distinctive evolutions in their geometric shapes at late growth stages. Volumes of MCTS were accurately quantified using a voxel-based approach without presumptions of their geometries. In contrast, conventional diameter-based volume calculations assuming perfect spherical shape resulted in large quantification errors. Furthermore, we successfully detected necrotic regions within these tumor spheroids based on increased intrinsic optical attenuation, suggesting a promising alternative of label-free viability tests in tumor spheroids. Therefore, OCT can serve as a promising imaging modality to characterize morphological and physiological features of MCTS, showing great potential for high-throughput drug screening.

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Extracting relevant information for cancer diagnosis from dynamic full field OCT through image processing and learning

Cited by 7 publications

References 6 publications

Ex Vivo Microscopy: A Promising Next-Generation Digital Microscopy Tool for Surgical Pathology Practice

Ex Vivo Microscopy: A Promising Next-Generation Digital Microscopy Tool for Surgical Pathology Practice

Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography

Optical Coherence Tomography Detects Necrotic Regions and Volumetrically Quantifies Multicellular Tumor Spheroids

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