Extract-free immunochromatographic assay for on-site tests of aflatoxin M1 in milk

Zhang, Dao H.; Li, Pei W.; Zhang, Qi; Yang, Yang; Zhang, Wen; Guan, Di; Ding, Xiao

doi:10.1039/c2ay25205h

Cited by 27 publications

(14 citation statements)

References 43 publications

(39 reference statements)

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“…Moreover, the intensity of the protein absorption at 280 nm increased after centrifugation ( Figures 3B, 3D, 3F). This result is comparable with the reports of Zhao et al (2010) and Zhang et al (2012), showing a decrease of the intensity of absorbance at 280 nm caused by removal of the excess unbound Abs or BSA after the centrifugal step. Excessive proteins could not be removed, but their presence in the conjugate solution had no negative effect on the test specificity and sensitivity.…”

Section: Synthesis Of Gold Nanoparticles and Conjugation With Antibodiessupporting

confidence: 92%

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Çam

Öktem

2017

Turk J Biol

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IntroductionMany strains of Escherichia coli bacteria live in the gastrointestinal tracts of humans and animals. E. coli O157 was reported as a food pathogen after the hemorrhagic colitis outbreak of 1982 (Riley et al., 1983). O157:H7 is one of the most important E. coli strains transmitted from cattle/ animals to humans (Dorn and Angrick, 1991;Altekruse et al., 1997;Slutsker et al., 1998), either by contact, eating contaminated foods, drinking contaminated water, or passing from one person to another directly (Heiman et al., 2015). Infections of humans with E. coli O157:H7 can result in clinical issues like hemolytic uremic syndrome, acute nonbloody diarrhea, and thrombocytopenic thrombotic purpura. A comparison of the outbreaks of E. coli O157:H7 during 2003 and 2012 showed their abundance recently in the United States (Heiman et al., 2015). Hospitalizations and infections caused by E. coli O157 are still being seen, according to food safety news.Since pathogenic E. coli contaminates food and water easily (Liu and Li, 2002) and causes severe defects in humans and animals, its early detection is crucial for public health. Therefore, many kinds of detection platforms, like real-time PCR (

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Section: Synthesis Of Gold Nanoparticles and Conjugation With Antibodiessupporting

confidence: 92%

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Çam

Öktem

2017

Turk J Biol

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“…Aflatoxins are scarcely extracted by hexane , and it is an effective method for removing lipids. In addition, several studies have used emulsifiers for aflatoxin analysis (Sadia et al, 2012;Zhang et al, 2012).…”

Section: Comparing Analytical Methods' Validation Values and Optimizamentioning

confidence: 99%

Analysis of aflatoxin M1 and M2 in commercial dairy products using high-performance liquid chromatography with a fluorescence detector

Lee

2015

Food Control

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“…The cross-reaction rates for AFB 1 , AFB 2 and AFG 1 were less than 1%. The proposed method was more sensitive than the ELISA (Jiang et al, 2013;Pei, Zhang, Eremin, & Lee, 2009) and the immunochromatographic assay (Anfossi et al, 2013;Zhang et al, 2012). The CLEIA method has been successfully applied for the determination of AFM 1 in milk products and the results correlated well with the accredited ELISA method (y = 0.02x + 0.898: r = 0.9978).…”

Section: Sample Analysismentioning

confidence: 80%

Development of chemiluminescent enzyme immunoassay for the determination of aflatoxin M₁in milk products

Zhang

Lei

et al. 2014

Food and Agricultural Immunology

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In this study, an indirect competitive chemiluminescent enzyme immunoassay (ic-CLEIA) for determining aflatoxin M 1 (AFM 1) in milk products has been developed. A luminol-hydrogen peroxide chemiluminescence system catalysed by horseradish peroxidase (HRP) was used as the signal detecting system. The effects of several factors, including concentration and pH of phosphate buffer, dilution ratio of antibody and antigen and other relevant variables on the immunoassay, were studied and optimised by single-factor experiments. The developed method presented an IC 50 of 0.05 ng/mL, a detection limit of 0.01 ng/mL and a linear range from 0.018 to 0.13 ng/mL. This method has been successfully applied to the evaluation of AFM 1 in milk products, the recoveries ranging from 71.9% to 109.0%. A good correlation with the commercial available ELISA kit for AFM 1 (r = 0.9978) was obtained, indicating that the ic-CLEIA method developed can be used to determine AFM 1 in real samples.

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Extract-free immunochromatographic assay for on-site tests of aflatoxin M1 in milk

Cited by 27 publications

References 43 publications

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Analysis of aflatoxin M1 and M2 in commercial dairy products using high-performance liquid chromatography with a fluorescence detector

Development of chemiluminescent enzyme immunoassay for the determination of aflatoxin M₁in milk products

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Extract-free immunochromatographic assay for on-site tests of aflatoxin M1 in milk

Cited by 27 publications

References 43 publications

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Analysis of aflatoxin M1 and M2 in commercial dairy products using high-performance liquid chromatography with a fluorescence detector

Development of chemiluminescent enzyme immunoassay for the determination of aflatoxin M1in milk products

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Development of chemiluminescent enzyme immunoassay for the determination of aflatoxin M₁in milk products