Extracorporeal Perfusion of Excised Canine Small Bowel Segments

Grenier, J F; Dauchel, J; Eloy, M.R.

doi:10.1159/000197526

Cited by 3 publications

(2 citation statements)

References 15 publications

(29 reference statements)

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“…In our experiments, the stabili ty of oxygen saturation in the venous effluent and of the calculated oxygen consumption demonstrated tissue via bility. In comparison with our results, other preparations utilizing red blood cells showed similar results [10,14,20] whereas lower oxygen consumption was measured using artifical oxygen carriers [16]. In accordance with our bio chemical viability parameters, the histology after 60 min revealed no obvious tissue damage.…”

Section: Discussionsupporting

confidence: 89%

Studies on the Viability of the Isolated Vascularly Perfused Rat Colon

Herrmann-Rinke¹,

Eissele²,

Arnold³

et al. 1996

Digestion

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The colon contains large numbers of endocrine cells. Insight into their physiological function is limited. This is due to the fact that no sufficient model of isolated endocrine colon cells is available. In the present study we introduce an isolated vascularly perfused colon model for in vitro studies. This model offers the advantage that it keeps the endocrine cells in their physiological orientation and environment. The gut mucosa is highly sensitive to ischemia. Therefore, a careful validation of its viability is crucial in gut organ preparations. This study demonstrates that, by utilizing an oxygenated vascular medium supplemented with 25% washed bovine erythrocytes, a perfusion of the colon is achieved for at least 1 h without obvious tissue injuries. During this time parameters such as perfusion pressure, venous lactate dehydrogenase release, glucose consumption, lactate output, oxygen consumption, perfusate loss by the preparation, and morphology were analyzed. Dependent on stimulation, the endocrine L cells of the colon released glucagon-like peptide-I upon arterial perfusion of methacholine or gastrin-releasing peptide. In conclusion, a model for the isolated perfusion of the colon is introduced which is suitable for studies of endocrine colon cells.

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Section: Discussionsupporting

confidence: 89%

Studies on the Viability of the Isolated Vascularly Perfused Rat Colon

Herrmann-Rinke¹,

Eissele²,

Arnold³

et al. 1996

Digestion

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“…A large number of experiments to study intestinal physiology was carried out in the 1960s to early 1980s. Most of the experimental protocols used large animals such as dogs and cats [10][11][12]. When the rat model was used, either in situ perfusion [13], xenogeneic blood cells as perfusate [3,14], oxygenated buffer solution as perfusate [15], or non-recirculating preparations [16] were used.…”

Section: Discussionmentioning

confidence: 99%

Computer-Assisted ex vivo, Normothermic Small Bowel Perfusion

Stangl

J²,

Theodorou³

et al. 2000

Eur Surg Res

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Background: In the present study, a technique for computer-assisted, normothermic, oxygenated, ex vivo, recirculating small bowel perfusion was established as a tool to investigate organ pretreatment protocols and ischemia/reperfusion phenomena. A prerequisite for the desired setup was an organ chamber for ex vivo perfusion and the use of syngeneic whole blood as perfusate. Methods: The entire small bowel was harvested from Lewis rats and perfused in an organ chamber ex vivo for at least 2 h. The temperature was kept at 37°C in a water bath. Three experimental groups were explored, characterized by different perfusion solutions. The basic perfusate consisted of syngeneic whole blood diluted with either NaCl, Krebs’ solution or Krebs’ solution and norepinephrine to a hematocrit of 30%. In addition, in each group l-glutamine was administered intraluminally. The desired perfusion pressure was 100 mm Hg which was kept constant with a computer-assisted data acquisition software, which measured on-line pressure, oxygenation, flow, temperature and pH and adjusted the pressure by changing the flow via a peristaltic pump. The viability of the preparation was tested by measuring oxygen consumption and maltose absorption, which requires intact enzymes of the mucosal brush border to break down maltose into glucose. Results: Organ perfusion in group 1 (dilution with NaCl) revealed problems such as hypersecretion into the bowel lumen, low vascular resistance and no maltose uptake. In contrast a viable organ could be demonstrated using Krebs’ solution as dilution solution. The addition of norepinephrine led to an improved perfusion over the entire perfusion period. Maltose absorption was comparable to tests conducted with native small bowel. Oxygen consumption was stable during the 2-hour perfusion period. Conclusions: The ex vivo perfusion system established enables small bowel perfusion for at least 2 h. The viability of the graft could be demonstrated. The perfusion time achieved is sufficient to study leukocyte/lymphocyte interaction with the endothelium of the graft vessels. In addition, a viable small bowel, after 2 h of ex vivo perfusion, facilitates testing of pretreatment protocols for the reduction of the immunogenicity of small bowel allografts.

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Influence of blood flow on intestinal absorption of drugs and nutrients

Wine

1979

Pharmacology & Therapeutics

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Extracorporeal Perfusion of Excised Canine Small Bowel Segments

Cited by 3 publications

References 15 publications

Studies on the Viability of the Isolated Vascularly Perfused Rat Colon

Studies on the Viability of the Isolated Vascularly Perfused Rat Colon

Computer-Assisted ex vivo, Normothermic Small Bowel Perfusion

Influence of blood flow on intestinal absorption of drugs and nutrients

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