Extracellular Vesicles in chondrogenesis and Cartilage regeneration

Zhao, Jiasong; Cheng, Jun; Zhou, Feng; Wang, Jianhua; Momtazi‐Borojeni, Amir Abbas; Liang, Yimin

doi:10.1111/jcmm.16290

Cited by 24 publications

(25 citation statements)

References 111 publications

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“…Many studies have recently examined the potential use of exosomes as diagnostic markers and gene carriers for therapeutic purposes [ 66 ]. Exosomes (30–150 nm in diameter) are divided into three types: EVs’ micro-vessels/heading particles and apoptotic bodies (≥100 nm) [ 67 ].…”

Section: Application Of 3d Spheroid Culture System To Chondrogenic Di...mentioning

confidence: 99%

“…MSC-exos have demonstrated excellent efficacy in tissue repair and reconstruction in a variety of organs [ 71 ]. Exosomes have been widely used by researchers for the treatment of autoimmune and inflammatory diseases such as osteoarthritis, because they play critical roles in the modulation of inflammation and immune responses (OA) ( Figure 4 ) [ 66 ]. However, large quantities of MSC-exos are required for preclinical and clinical research on exosome-based therapy.…”

Section: Application Of 3d Spheroid Culture System To Chondrogenic Di...mentioning

confidence: 99%

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3D Spheroid Cultures of Stem Cells and Exosome Applications for Cartilage Repair

Lee

2022

Life

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Cartilage is a connective tissue that constitutes the structure of the body and consists of chondrocytes that produce considerable collagenous extracellular matrix and plentiful ground substances, such as proteoglycan and elastin fibers. Self-repair is difficult when the cartilage is damaged because of insufficient blood supply, low cellularity, and limited progenitor cell numbers. Therefore, three-dimensional (3D) culture systems, including pellet culture, hanging droplets, liquid overlays, self-injury, and spinner culture, have attracted attention. In particular, 3D spheroid culture strategies can enhance the yield of exosome production of mesenchymal stem cells (MSCs) when compared to two-dimensional culture, and can improve cellular restorative function by enhancing the paracrine effects of MSCs. Exosomes are membrane-bound extracellular vesicles, which are intercellular communication systems that carry RNAs and proteins. Information transfer affects the phenotype of recipient cells. MSC-derived exosomes can facilitate cartilage repair by promoting chondrogenic differentiation and proliferation. In this article, we reviewed recent major advances in the application of 3D culture techniques, cartilage regeneration with stem cells using 3D spheroid culture system, the effect of exosomes on chondrogenic differentiation, and chondrogenic-specific markers related to stem cell derived exosomes. Furthermore, the utilization of MSC-derived exosomes to enhance chondrogenic differentiation for osteoarthritis is discussed. If more mechanistic studies at the molecular level are conducted, MSC-spheroid-derived exosomes will supply a better therapeutic option to improve osteoarthritis.

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Section: Application Of 3d Spheroid Culture System To Chondrogenic Di...mentioning

confidence: 99%

Section: Application Of 3d Spheroid Culture System To Chondrogenic Di...mentioning

confidence: 99%

3D Spheroid Cultures of Stem Cells and Exosome Applications for Cartilage Repair

Lee

2022

Life

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“…The above-mentioned MSCs and non-MSCs secreted EVs potentiate miRNAs-mediated epigenetic regulation in OA. Significantly, these studies have demonstrated the vital role of EVs-mediated miRNAs in cartilage destruction and regeneration, 20 synovial inflammation, 38 bone remodeling, 83 and the crosstalk among the above biological processes. 37 , 40 , 75 Therefore, this may provide an important target to observe the pathological process of OA and even a crucial breakthrough to the treatment of OA.…”

Section: The Application Of Evs-mirnas In Oa Treatmentmentioning

confidence: 99%

The Application of Extracellular Vesicles Mediated miRNAs in Osteoarthritis: Current Knowledge and Perspective

Shang¹,

Yan²,

Yang³

et al. 2022

JIR

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Osteoarthritis (OA) is a whole joint disease characterized by synovitis, cartilage destruction, and subchondral bone sclerosis and cyst. Despite decades’ study, effective treatment is rare for this chronic disease. Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptosis bodies, are nano-sized vesicles with a cargo containing biologically active agents, such as nucleic acids, lipids, and proteins. As a group of short non-coding RNAs, microRNAs (miRNAs) can be delivered by parental cells secreted EVs. Negatively regulate the target mRNAs at the posttranscriptional level and regulate gene expression in recipient cells without modifying gene sequence. Recently, most studies focused on the function of EVs mediated miRNAs in the pathophysiological process of OA. However, all kinds of EVs specific and OA specific factors might influence the administration of EVs-miRNAs, especially the precise quantitative management. As a result, the flourishing of current research about EVs in the laboratory might not promote the relevant clinical transformation in OA treatment. In this review, we reviewed the present application of EVs-miRNAs in the therapeutic of OA and further analyzed the potential factors that might influence its application. Further progress in the quantitative management of EVs-miRNAs would accelerate the clinical transformation of miRNAs enriched EVs in the OA field.

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“…Nevertheless, it is beyond doubt that inflammatory processes in the synovial membrane play a crucial role in the initiation of OA and in promoting subsequent cartilage damage and pain at least in the inflammation-driven subset of patients ( de Lange-Brokaar et al, 2012 ). Therefore, the previously suggested differentiation potential of MSC into chondrocytes aiding cartilage regeneration ( Satué et al, 2019 ; Song et al, 2021 ) was pushed into the background against the ability of the MSC to target local immunocompetent cells.…”

Section: Targets and Mechanisms Of Msc In Tendon And Joint Diseasementioning

confidence: 99%

MSC in Tendon and Joint Disease: The Context-Sensitive Link Between Targets and Therapeutic Mechanisms

Roth¹,

Burk

Brehm³

et al. 2022

Front. Bioeng. Biotechnol.

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Mesenchymal stromal cells (MSC) represent a promising treatment option for tendon disorders and joint diseases, primarily osteoarthritis. Since MSC are highly context-sensitive to their microenvironment, their therapeutic efficacy is influenced by their tissue-specific pathologically altered targets. These include not only cellular components, such as resident cells and invading immunocompetent cells, but also components of the tissue-characteristic extracellular matrix. Although numerous in vitro models have already shown potential MSC-related mechanisms of action in tendon and joint diseases, only a limited number reflect the disease-specific microenvironment and allow conclusions about well-directed MSC-based therapies for injured tendon and joint-associated tissues. In both injured tissue types, inflammatory processes play a pivotal pathophysiological role. In this context, MSC-mediated macrophage modulation seems to be an important mode of action across these tissues. Additional target cells of MSC applied in tendon and joint disorders include tenocytes, synoviocytes as well as other invading and resident immune cells. It remains of critical importance whether the context-sensitive interplay between MSC and tissue- and disease-specific targets results in an overall promotion or inhibition of the desired therapeutic effects. This review presents the authors’ viewpoint on disease-related targets of MSC therapeutically applied in tendon and joint diseases, focusing on the equine patient as valid animal model.

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Extracellular Vesicles in chondrogenesis and Cartilage regeneration

Cited by 24 publications

References 111 publications

3D Spheroid Cultures of Stem Cells and Exosome Applications for Cartilage Repair

3D Spheroid Cultures of Stem Cells and Exosome Applications for Cartilage Repair

The Application of Extracellular Vesicles Mediated miRNAs in Osteoarthritis: Current Knowledge and Perspective

MSC in Tendon and Joint Disease: The Context-Sensitive Link Between Targets and Therapeutic Mechanisms

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