Extracellular Vesicles in Cardiovascular Diseases: Diagnosis and Therapy

Zhang, Xiaojing; Wu, Yuping; Cheng, Qifa; Bai, Liyang; Huang, Shuqiang; Gao, Jun

doi:10.3389/fcell.2022.875376

Cited by 11 publications

(10 citation statements)

References 126 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EVs are nanometer-sized membrane-enclosed particles that can transport cargo between cells and organ systems [ 54 ]. EVs were shown to have therapeutic potential [ 55 ] in several diseases, including cancer [ 56 ], neurological [ 57 ], and cardiovascular diseases [ 58 ]. The advantages of using EVs as drug vehicles for the central nervous system (CNS) are (i) their size, (ii) their stability in circulation, (iii) their ability to cross biological barriers such as the BBB, (iv) their ability to utilize endogenous cellular uptake mechanisms, and (v) their low immunogenicity [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult in vitro and in vivo

Gui,

Kim,

Brenna

et al. 2024

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Synaptic loss is an early event in the penumbra area after an ischemic stroke. Promoting synaptic preservation in this area would likely improve functional neurological recovery. We aimed to detect proteins involved in endogenous protection mechanisms of synapses in the penumbra after stroke and to analyse potential beneficial effects of these candidates for a prospective stroke treatment. For this, we performed Liquid Chromatography coupled to Mass Spectrometry (LC–MS)-based proteomics of synaptosomes isolated from the ipsilateral hemispheres of mice subjected to experimental stroke at different time points (24 h, 4 and 7 days) and compared them to sham-operated mice. Proteomic analyses indicated that, among the differentially expressed proteins between the two groups, cystatin C (CysC) was significantly increased at 24 h and 4 days following stroke, before returning to steady-state levels at 7 days, thus indicating a potential transient and intrinsic rescue mechanism attempt of neurons. When CysC was applied to primary neuronal cultures subjected to an in vitro model of ischemic damage, this treatment significantly improved the preservation of synaptic structures. Notably, similar effects were observed when CysC was loaded into brain-derived extracellular vesicles (BDEVs). Finally, when CysC contained in BDEVs was administered intracerebroventricularly to stroked mice, it significantly increased the expression of synaptic markers such as SNAP25, Homer-1, and NCAM in the penumbra area compared to the group supplied with empty BDEVs. Thus, we show that CysC-loaded BDEVs promote synaptic protection after ischemic damage in vitro and in vivo, opening the possibility of a therapeutic use in stroke patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult in vitro and in vivo

Gui,

Kim,

Brenna

et al. 2024

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

show abstract

“…Secreted factors and nucleic acids may also act as paracrine cues within and between regions of tissue, while hormones can act through bodily circulation in an endocrine manner. The role of extracellular vesicles (EVs) including exosomes has become increasingly recognized as an important mode of signal transmission between cell populations [ 31 , 32 ]. Thus, many efforts have been made to unravel the signaling pathways underlying heart development.…”

Section: Signaling Pathways In Heart Developmentmentioning

confidence: 99%

Differentiation of Pluripotent Stem Cells for Disease Modeling: Learning from Heart Development

Chi,

Roland,

Song

2024

Pharmaceuticals

View full text Add to dashboard Cite

Heart disease is a pressing public health problem and the leading cause of death worldwide. The heart is the first organ to gain function during embryogenesis in mammals. Heart development involves cell determination, expansion, migration, and crosstalk, which are orchestrated by numerous signaling pathways, such as the Wnt, TGF-β, IGF, and Retinoic acid signaling pathways. Human-induced pluripotent stem cell-based platforms are emerging as promising approaches for modeling heart disease in vitro. Understanding the signaling pathways that are essential for cardiac development has shed light on the molecular mechanisms of congenital heart defects and postnatal heart diseases, significantly advancing stem cell-based platforms to model heart diseases. This review summarizes signaling pathways that are crucial for heart development and discusses how these findings improve the strategies for modeling human heart disease in vitro.

show abstract

“…Indeed, recent years of studies have brought mounting evidence on protective and pro-regenerative capability of SC-derived EVs in the treatment of AMI and other CVD-related conditions. Thus, due to the unsatisfactory results of cell-based approaches, there has been an increased focus on the alternative solutions, including those related to the utilization of EVs that not only mimic several functional properties of cells of their origin, but also are non-tumorigenic, easy to be stored and may penetrate biological barriers more effectively than the whole cells ( 125 ).…”

Section: Scs-evs As An Alternative Option To Cell-based Therapiesmentioning

confidence: 99%

Stem cell- derived extracellular vesicles as new tools in regenerative medicine - Immunomodulatory role and future perspectives

2023

View full text Add to dashboard Cite

In the last few decades, the practical use of stem cells (SCs) in the clinic has attracted significant attention in the regenerative medicine due to the ability of these cells to proliferate and differentiate into other cell types. However, recent findings have demonstrated that the therapeutic capacity of SCs may also be mediated by their ability to secrete biologically active factors, including extracellular vesicles (EVs). Such submicron circular membrane-enveloped vesicles may be released from the cell surface and harbour bioactive cargo in the form of proteins, lipids, mRNA, miRNA, and other regulatory factors. Notably, growing evidence has indicated that EVs may transfer their bioactive content into recipient cells and greatly modulate their functional fate. Thus, they have been recently envisioned as a new class of paracrine factors in cell-to-cell communication. Importantly, EVs may modulate the activity of immune system, playing an important role in the regulation of inflammation, exhibiting broad spectrum of the immunomodulatory activity that promotes the transition from pro-inflammatory to pro-regenerative environment in the site of tissue injury. Consequently, growing interest is placed on attempts to utilize EVs in clinical applications of inflammatory-related dysfunctions as potential next-generation therapeutic factors, alternative to cell-based approaches. In this review we will discuss the current knowledge on the biological properties of SC-derived EVs, with special focus on their role in the regulation of inflammatory response. We will also address recent findings on the immunomodulatory and pro-regenerative activity of EVs in several disease models, including in vitro and in vivo preclinical, as well as clinical studies. Finally, we will highlight the current perspectives and future challenges of emerging EV-based therapeutic strategies of inflammation-related diseases treatment.

show abstract

Extracellular Vesicles in Cardiovascular Diseases: Diagnosis and Therapy

Cited by 11 publications

References 126 publications

Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult in vitro and in vivo

Cystatin C loaded in brain-derived extracellular vesicles rescues synapses after ischemic insult in vitro and in vivo

Differentiation of Pluripotent Stem Cells for Disease Modeling: Learning from Heart Development

Stem cell- derived extracellular vesicles as new tools in regenerative medicine - Immunomodulatory role and future perspectives

Contact Info

Product

Resources

About