Extracellular vesicles from adipose stromal cells combined with a thermoresponsive hydrogel prevent esophageal stricture after extensive endoscopic submucosal dissection in a porcine model

Abstract

We show herein that a combination of a biomaterial and extracellular vesicles (produced at a high yield by a turbulence stimulation from stromal cells) was effective in the prevention of esophageal stricture in a clinically relevant porcine model.

Poly(lactic acid)-hyperbranched polyglycerol nanoparticles enhance bioadhesive treatment of esophageal disease and reduce systemic drug exposure

“…For this reason, large solid dosage forms may be less suitable for patients at risk of esophageal strictures. 10,11 Whilst stents are often offered to patients with strictures, whereby the esophageal lumen is opened using a tube or scaffold, [12][13][14] these interventions are invasive and commonly cause complications, such as recurrent dysphagia, pain, and infection. 15,16 Consequently, non-invasive treatments for esophageal disease are highly sought.…”

Click‐hydrogel delivered aggregation‐induced emissive nanovesicles for simultaneous remodeling and antibiosis of deep burn wounds

“…[7] And adipose-derived stem cells (ADSCs) which can be extracted from adult adipose tissue and expanded in vitro are the optimal choice with abundant supply. [8] However, the clinical promotion of cell transplantation is still hindered by low survival rates and potential safety risks associated with tumor cell metastasis and organ damage. [9][10][11] Consequently, stem cell-derived extracellular vesicles (EVs) with beneficial cytokines have gained significant attention as biomaterials for wound repair attributed to their superior safety, tissue penetration ability, and drug carrying capacity.…”

“…[29,30] However, hydrogels for controllable release are usually prepared using UV light, heating, or catalysts, which may potentially harm the essential biological components of nanovesicles. [31] Although temperature-sensitive hydrogel PF-127 has been utilized for in situ gelation in esophagus without affecting the functions of nanovesicles, [32] it is not suitable for skin with unstable temperatures.…”

Click-Chemistry Hydrogel Delivery Aggregation-Induced Emission-Active Nanovesicles Enables “One-Stop” Remodeling and Antibiosis on Deep Scald Wound

“…[26,27] However, the preparation of controllable release hydrogels commonly requires UV light, heating or catalyst for gelation, which might harm the beneficial biological components of nanovesicles. [28] Temperature sensitive hydrogel PF-127 have been utilized for in situ gelation on wounds without affecting the functions of nanovesicles, [29] but it is more suitable for a constant temperature internal environment rather than on the surface of the skin. Scheme 1.…”

“…Adipose-derived stem cells (ADSCs), which can be easily obtained from adipose tissues, have been shown to exert protective effects in several diseases through the exosomes they release. [31] Therefore, we chose ADSCs as our material source for preparing ANVs using a simple extrusion method, with the aim of mimicking the functions of ADSC exosomes. [16] As shown in Figure 1A (left), ANVs display a tea cup holder-like structure with a size of 100-200 nm, as observed by transmission electron microscopy (TEM).…”