Click-Chemistry Hydrogel Delivery Aggregation-Induced Emission-Active Nanovesicles Enables “One-Stop” Remodeling and Antibiosis on Deep Scald Wound

Abstract

The serve burn or scald wounds always face persistent infections and self-repair function decline caused by serious tissue necrosis, leading to delayed healing or even sepsis. In this work, we proposed a click-chemistry hydrogel delivery system of antibacterial and tissue remodeling function nanovesicles for deep scald wound treatment. An hydrophilic photodynamic aggregation-induced emission photosensitizer 4-(2-(5-(4-(diphenylamino)phenyl)thiophen-2-yl)vinyl)-1-(2-hydroxyethyl) pyridin-1-ium bromide (THB) was firstly encapsulated into nanovesicles derived from easily accessible adipose stem cells (ANVs) to create THB@ANVs, which exhibits enhanced bacteria-targeting property and multiple tissue remodeling effects. To deliver THB@ANVs, an injectable click-chemistry hydrogel of carboxymethyl chitosan was used to form a wound treatment system for deep scald wounds. The hydrogel can well matching the wound morphology and respond to the acidic microenvironment of the wound to accelerate sustained release. In vivo wound healing evaluations show that the composite hydrogel can efficiently accelerate wound healing by reducing the number of bacteria, promoting early angiogenesis, and regulating immune reaction. This study provides a simple, low-cost, and effective “one-stop” strategy with diverse functions and wide applicability for scald wound remodeling and antibiosis.