Extracellular production of palmitoleic triglycerides by a yeast, Trichosporon

Yagi, Takashi; Hatano, Akiko; Nakanishi, Taisuke; Hatano, Takushi; Fukui, Sakuzô

doi:10.1016/0922-338x(94)90317-4

Cited by 8 publications

(6 citation statements)

References 7 publications

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“…The strain YP1 was mutagenized with 3.0% ethylmethane sulfonate (EMS, Aldrich, Milwaukee, WIS, USA) and spread on the YPAD plate. After a 3-day incubation, colonies were overlaid with YNBD agar containing 1ϫ10 7 cells/ml of KD115 and lipase, followed by further incubation for 18 h. Extracellular TG accumulator allows to grow the tester strain (KD115) and can be selected as a "halo-giving" colony (Yagi et al, 1994). Halo becomes visible because of the formation of tester microcolonies.…”

Section: Methodsmentioning

confidence: 99%

“…The extracts were recovered by centrifugation and collected as intracellular lipids. The qualities of extra-and intracellular lipids were analyzed by TLC (Yagi et al, 1994). The TG fraction separated on a TLC plate was then recovered from silica gel by extraction with chloroform : methanolϭ1 : 1 (v/v).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore processes for cell disruption and oil extraction were essential to recover such products as other oleaginous plants or animals. The development of extracellular accumulation of fats (triacylglycerols, TGs) must be promising for an improvement in the cost performance of microbial lipid fermentation.Previously we reported the breeding of mutant strains of yeast Candida lipolytica that secrete palmitic acid into glucose medium (Miyakawa et al, 1984), and of the yeast Trichosporon sp., which accumulates TGs in the ethylpalmitate (Yagi et al, 1994) or glucose (Nojima et al, 1995) medium. As far as we know, no other reports exist concerning the extracellular production of useful lipids by microorganisms.…”

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confidence: 99%

“…Previously we reported the breeding of mutant strains of yeast Candida lipolytica that secrete palmitic acid into glucose medium (Miyakawa et al, 1984), and of the yeast Trichosporon sp., which accumulates TGs in the ethylpalmitate (Yagi et al, 1994) or glucose (Nojima et al, 1995) medium. As far as we know, no other reports exist concerning the extracellular production of useful lipids by microorganisms.…”

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confidence: 99%

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Isolation and characterization of triacylglycerol-secreting mutant strain from yeast, Saccharomyces cerevisiae.

Nojima

Kibayashi

Matsuzaki

et al. 1999

J. Gen. Appl. Microbiol.

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In recent times, demands for bio-oils, such as biolipids and biofuels, have progressed, since they are very significant biomolecules for human health and for the ecological preservation of the earth's environment. Nevertheless, the supplements of bio-oils were not always stable because the harvests of oleaginous plants, animals, and fishes were influenced by climates and marine conditions. Various research and developments were carried out on microbial production of valuable oils, such as polyunsaturated fatty acids, from the viewpoint of factory-controllable fermentative formation of specified products. So far, it has been reported on the production of eicosapentaenoic acid (Shimizu et al., 1988), docosahexaenoic acid (Li and Ward, 1994), and g-linolenic acid (Hansson and Dostalek, 1988) using various oleaginous fungi. These products, however, were entirely localized at the inner space of cell. Therefore processes for cell disruption and oil extraction were essential to recover such products as other oleaginous plants or animals. The development of extracellular accumulation of fats (triacylglycerols, TGs) must be promising for an improvement in the cost performance of microbial lipid fermentation.Previously we reported the breeding of mutant strains of yeast Candida lipolytica that secrete palmitic acid into glucose medium (Miyakawa et al., 1984), and of the yeast Trichosporon sp., which accumulates TGs in the ethylpalmitate (Yagi et al., 1994) or glucose (Nojima et al., 1995) medium. As far as we know, no other reports exist concerning the extracellular production of useful lipids by microorganisms. We could exhibit the construction of lipid-secretable microorganisms, though their productivities were economically insufficient. For further improvement of the capacity to secrete lipid, it would be necessary to have not only a screening of a superior producer, but also an analysis of the molecular mechanism for the outward flow of lipids. We would advocate that the extracellular accumulation of hydrophobic biomaterials by the fermentation process has a higher probability to be realized if the equilibrium of membrane transport for hydrophobic compounds could be made up to incline toward pumping out. It is widely known that the yeast Saccharomyces cerevisiae is one of the most widely studied microorganisms in molecular genetics. Studies on lipid-excreting mechanisms using a mutant strain of S. cerevisiae should have advantages to breed useful microorganisms for lipid-accumulating fermentation. In this paper we describe isolation and characterization of the S. cerevisiae mutant strain that secretes TG into glucose medium. Moreover, we represent compleIsolation and characterization of triacylglycerol-secreting mutant strain from yeast, Saccharomyces cerevisiae Department of Bioscience and Biotechnology, Faculty of Engineering, Fukuyama University, Fukuyama 729-0292, Japan (Received September 11, 1998; Accepted January 7, 1999) To establish the molecular bases for development of a microbiological system approach...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation and characterization of triacylglycerol-secreting mutant strain from yeast, Saccharomyces cerevisiae.

Nojima

Kibayashi

Matsuzaki

et al. 1999

J. Gen. Appl. Microbiol.

Self Cite

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“…Cultivation was performed at 28°C with shaking (120 strokes/min). After a 4-day incubation, products accumulated in the intra-and extracellular space were extracted and analyzed by TLC and HPLC (Nojima et al, 1995;Yagi et al, 1994). Table 1 shows the growth of cells and the extracellular production of TGs on various substrates.…”

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confidence: 99%