Extracellular formation of triglycerides from glucose by a mutant strain of Trichosporon

Fatty acid ethyl esters production in aqueous phase by the oleaginous yeast Rhodosporidium toruloides

“…The neutral lipids included TAGs, diglycerides (DAGs), monoglycerides (MAGs), FFAs and FAEEs. Lipid fractions were also analyzed by thin layer chromatography (TLC) using a mixture of n-hexane, diethyl ether and acetic acid (80:20:1, vol/vol/vol) as the developing agent (Nojima et al, 1995).…”

Substrate specificities in triacylglycerol-secretion by the yeast, Trichosporon sp.

“…The accumulation of extra-and intracellular TGs has not arrested the cell growth of both strains. The middle chain fatty acids were not detectable in the constituents of TGs formed on glucose (Nojima et al, 1995). The activities of lipase, also, were hardly detected in either medium.…”

“…For an increased production of palmitoleic TGs from ethyl palmitate, we have further obtained the strain E228 by the screening, using an unsaturated fatty acid-auxotrophic yeast strain as a tester , after mutagenesis on E2. Furthermore, we have bred the novel mutant strain of Trichosporon sp., L-12, which excretes TGs from glucose as the substrate for growth (Nojima et al, 1995). In this communication, we studied substrate specificities in the excretive production of TGs by the mutant strains of yeast Trichosporon sp.…”

“…1, 2). The measurement of glucose in the medium was performed by HPLC (Nojima et al, 1995). The substrates were con- …”

Isolation and characterization of triacylglycerol-secreting mutant strain from yeast, Saccharomyces cerevisiae.

“…Therefore processes for cell disruption and oil extraction were essential to recover such products as other oleaginous plants or animals. The development of extracellular accumulation of fats (triacylglycerols, TGs) must be promising for an improvement in the cost performance of microbial lipid fermentation.Previously we reported the breeding of mutant strains of yeast Candida lipolytica that secrete palmitic acid into glucose medium (Miyakawa et al, 1984), and of the yeast Trichosporon sp., which accumulates TGs in the ethylpalmitate (Yagi et al, 1994) or glucose (Nojima et al, 1995) medium. As far as we know, no other reports exist concerning the extracellular production of useful lipids by microorganisms.…”