Extracellular Polysaccharide-Degrading Proteome of <i>Butyrivibrio proteoclasticus</i>

Dunne, Jonathan C.; Liu, Dong; Kelly, William J.; Leahy, Sinead C.; Bond, J. J.; Attwood, Graeme T.; Jordan, T. William

doi:10.1021/pr200864j

Cited by 22 publications

(35 citation statements)

References 56 publications

(86 reference statements)

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“…Therefore, even if the different methods and different ruminant breeds were used, Butyrivibrio dominated the stomach of ruminants. Dunne et al demonstrated that Butyrivibrio could modulate the secretion of hemicelluloses-degrading enzymes [22], which supported the notion that this organism made an important contribution to polysaccharide degradation in the rumen. In addition, Butyrivibrio is an important butyrate producer, and promotes the stomach epithelium proliferation.…”

Section: Discussionmentioning

confidence: 91%

Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis

Wang

Fan

Han

et al. 2016

Asian-Australas J Anim Sci

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ObjectiveThe gastrointestinal tract of sheep contain complex microbial communities that influence numerous aspects of the sheep’s health and development. The objective of this study was to analyze the composition and diversity of the microbiota in the gastrointestinal tract sections (rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum, colon, and rectum) of sheep.MethodsThis analysis was performed by 454 pyrosequencing using the V3-V6 region of the 16S rRNA genes. Samples were collected from five healthy, small tailed Han sheep aged 10 months, obtained at market. The bacterial composition of sheep gastrointestinal microbiota was investigated at the phylum, class, order, family, genus, and species levels.ResultsThe dominant bacterial phyla in the entire gastrointestinal sections were Firmicutes, Bacteroidetes, and Proteobacteria. In the stomach, the three most dominant genera in the sheep were Prevotella, unclassified Lachnospiraceae, and Butyrivibrio. In the small intestine, the three most dominant genera in the sheep were Escherichia, unclassified Lachnospiraceae, and Ruminococcus. In the large intestine, the three most dominant genera in the sheep were Ruminococcus, unclassified Ruminococcaceae, and Prevotella. R. flavefaciens, B. fibrisolvens, and S. ruminantium were three most dominant species in the sheep gastrointestinal tract. Principal Coordinates Analysis showed that the microbial communities from each gastrointestinal section could be separated into three groups according to similarity of community composition: stomach (rumen, reticulum, omasum, and abomasum), small intestine (duodenum, jejunum, and ileum), and large intestine (cecum, colon, and rectum).ConclusionThis is the first study to characterize the entire gastrointestinal microbiota in sheep by use of 16S rRNA gene amplicon pyrosequencing, expanding our knowledge of the gastrointestinal bacterial community of sheep.

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Section: Discussionmentioning

confidence: 91%

Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis

Wang

Fan

Han

et al. 2016

Asian-Australas J Anim Sci

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“…The software TMHMM 2.0 and PSORT Version 3.0 were separately used to predict transmembrane domains and cellular localizations. Protein functions were assigned using Tigrfam and BLASTp [74]. …”

Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis

et al. 2014

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BackgroundHaemophilus parasuis is the causative agent of Glässer’s disease characterized by polyserositis, arthritis, and meningitis in pig, leading to serious economic loss. Despite many years of study, virulence factors and the mechanisms of the entire infection process remain largely unclear. So two-dimensional gel electrophoresis and mass spectrometry were used to search for distinctions at the membrane protein expression level between two H. parasuis isolates aimed at uncovering some proteins potentially involved in habitat adaption and pathogenesis.ResultsA comparative proteomic approach combining two-dimensional gel electrophoresis with mass spectrometry and tandem mass spectrometry was employed to explore the differences among membrane proteomes of a virulent Haemophilus parasuis strain isolated from the lung of a diseased pig and an avirulent strain isolated from the nasal swab of a healthy pig. Differentially expressed protein spots identified by mass spectrometry were annotated and analyzed by bioinformatic interpretation. The mRNA level was determined by quantitative real-time PCR. Proteins representing diverse functional activities were identified. Among them, the tonB-dependent siderophore receptor was a new discovery highlighted for its activity in iron uptake. In addition, periplasmic serine protease and putrescine/spermidine ABC transporter substrate-binding protein were given focus because of their virulence potential. This study revealed that the differentially expressed proteins were important in either the habitat adaption or pathogenesis of H. parasuis.ConclusionsThe outcome demonstrated the presence of some proteins which raise the speculation for their importance in helping in habitat adaption or pathogenesis within the host.

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“…Kelly et al [9] proposed a mechanism of extracellular polysaccharide breakdown whereby a group of nine cell-associated proteins that target xylan, pectin, and starch form the core of the extracellular catalytic potential. Recent examination by two-dimensional electrophoresis (2DE) of the extracellular polysaccharide-degrading proteome of B. proteoclasticus supported this mechanism [10], and demonstrated that at least four (Xyn10B, Xsa43J, Pme8B, and Pel1A) of the nine extracellular enzymes are secreted when cells are grown using xylan as the only hemicellulosic carbon source. The endo-1,4-β-xylanase Xyn10B was significantly more abundant in the culture medium of xylan-grown cells, which suggests it plays a primary role in B. proteoclasticus mediated hemicellulose degradation.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the secreted xylosidase/arabinofuranosidase Xsa43J is expected to hydrolyze xylobiose, arabinoxylans and arabinogalactans. Several ATP-binding cassette (ABC) transporter substrate-binding proteins were also found to be significantly more abundant in the xylan-grown cell culture medium [10], and in the membrane proteome [13], which implies they are important for uptake of oligosaccharides derived from xylan disassembly.…”

Section: Introductionmentioning

confidence: 99%

“…Based on these results the proposed model of hemicellulose degradation and assimilation by B. proteoclasticus suggests extracellular hydrolysis of the xylan backbone of hemicellulose followed by transport of substituted or un-substituted xylooligosaccharides into the cell for further metabolism [10]. This model necessitates that B. proteoclasticus possesses the intracellular catalytic machinery that allows cells to rapidly degrade and metabolize the internalized oligosaccharides.…”

Section: Introductionmentioning

confidence: 99%

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The Cytosolic Oligosaccharide-Degrading Proteome of Butyrivibrio Proteoclasticus

et al. 2015

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The growth and productivity of ruminants depends on a complex microbial community found in their fore-stomach (rumen), which is able to breakdown plant polysaccharides and ferment the released sugars. Butyrivibrio proteoclasticus B316T is a Gram-positive polysaccharide-degrading, butyrate-producing bacterium that is present at high numbers in the rumen of animals consuming pasture or grass silage based diets. B316T is one of a small number of rumen fibrolytic microbes capable of efficiently degrading and utilizing xylan, as well as being capable of utilizing arabinose, xylose, pectin and starch. We have therefore carried out a proteomic analysis of B316T to identify intracellular enzymes that are implicated in the metabolism of internalized xylan. Three hundred and ninety four proteins were identified including enzymes that have potential to metabolize assimilated products of extracellular xylan digestion. Identified enzymes included arabinosidases, esterases, an endoxylanase, and β-xylosidase. The presence of intracellular debranching enzymes indicated that some hemicellulosic side-chains may not be removed until oligosaccharides liberated by extracellular digestion have been assimilated by the cells. The results support a model of extracellular digestion of hemicellulose to oligosaccharides that are then transported to the cytoplasm for further digestion by intracellular enzymes.

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Extracellular Polysaccharide-Degrading Proteome of Butyrivibrio proteoclasticus

Cited by 22 publications

References 56 publications

Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis

Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis

Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis

The Cytosolic Oligosaccharide-Degrading Proteome of Butyrivibrio Proteoclasticus

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