Extracellular peptidases of insect-associated fungi and their possible use in biological control programs and as pathogenicity markers

Семенова, Т. А.; Dunaevsky, Yakov E.; Beljakova, Galina; Belozersky, M. A.

doi:10.1016/j.funbio.2019.11.005

Cited by 13 publications

(11 citation statements)

References 67 publications

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“…In the future, genetic engineering might also be applied to improve the resistance of entomopathogenic agents to abiotic stresses to modulate their host range or their sensitivity to chemical pesticides and fungicides (Bielza et al ., 2020 ). However, to achieve this, a better understanding of virulence factors (Gao et al ., 2020 ; Semenova et al ., 2020 ), fitness (Mou et al ., 2020 ) and host–pathogen interactions (Hussain, 2018 ; Yang et al ., 2014a ) is required.…”

Section: Harnessing Biocontrolmentioning

confidence: 99%

Insect pest management in the age of synthetic biology

Mateos-Fernández

Petek

Gerasymenko

et al. 2021

Plant Biotechnology Journal

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Summary Arthropod crop pests are responsible for 20% of global annual crop losses, a figure predicted to increase in a changing climate where the ranges of numerous species are projected to expand. At the same time, many insect species are beneficial, acting as pollinators and predators of pest species. For thousands of years, humans have used increasingly sophisticated chemical formulations to control insect pests but, as the scale of agriculture expanded to meet the needs of the global population, concerns about the negative impacts of agricultural practices on biodiversity have grown. While biological solutions, such as biological control agents and pheromones, have previously had relatively minor roles in pest management, biotechnology has opened the door to numerous new approaches for controlling insect pests. In this review, we look at how advances in synthetic biology and biotechnology are providing new options for pest control. We discuss emerging technologies for engineering resistant crops and insect populations and examine advances in biomanufacturing that are enabling the production of new products for pest control.

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Section: Harnessing Biocontrolmentioning

confidence: 99%

Insect pest management in the age of synthetic biology

Mateos-Fernández

Petek

Gerasymenko

et al. 2021

Plant Biotechnology Journal

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“…Lipolytic enzymes including lipases, act primarily on the epicuticle, followed by proteases and chitinases, according to the presence of polymeric substrates in the different portions of the cuticle (Beys da Silva et al, 2010a,b). Among the proteases that can act at this level are subtilisins, trypsins, chymotrypsins, metallopeptidases, aspartyl peptidases, and exopeptidases (Semenova et al, 2020); where their expression from fungi such as M. anisopliae will depend specifically on the composition of the cuticle and hemolymph (Freimoser et al, 2005). EPF such as M. anisopliae and B. bassiana can express up to 11 different subtilisins, one of the most important being the Pr1 subtilisin-like peptidases, which intervene in the arthropod pathogenesis, causing hydrolysis of the cuticle and providing nutrients to the fungus (Gao et al, 2020;Semenova et al, 2020).…”

Section: Penetration Through the Host's Cuticlementioning

confidence: 99%

“…Among the proteases that can act at this level are subtilisins, trypsins, chymotrypsins, metallopeptidases, aspartyl peptidases, and exopeptidases (Semenova et al, 2020); where their expression from fungi such as M. anisopliae will depend specifically on the composition of the cuticle and hemolymph (Freimoser et al, 2005). EPF such as M. anisopliae and B. bassiana can express up to 11 different subtilisins, one of the most important being the Pr1 subtilisin-like peptidases, which intervene in the arthropod pathogenesis, causing hydrolysis of the cuticle and providing nutrients to the fungus (Gao et al, 2020;Semenova et al, 2020). To our knowledge, there are few reports elucidating the participation of these proteases during the infection process of cattle ticks (R. microplus, R. annulatus and A. mixtum) by EPF.…”

Section: Penetration Through the Host's Cuticlementioning

confidence: 99%

Entomopathogenic Fungi for Tick Control in Cattle Livestock From Mexico

Alonso-Díaz

Fernández-Salas

2021

Front. Fungal Biol.

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Ticks are one of the main economic threats to the cattle industry worldwide affecting productivity, health and welfare. The need for alternative methods to control tick populations is prompted by the high prevalence of multiresistant tick strains to the main chemical acaricides and their ecological consequences. Biological control using entomopathogenic fungi (EPF) is one of the most promising alternative options. The objective of this paper is to review the use of EPF as an alternative control method against cattle ticks in Mexico. Metarhizium anisopliae sensu lato (s.l.) and Beauveria bassiana s.l. are the most studied EPF for the biological control of ticks in the laboratory and in the field, mainly against Rhipicephalus microplus; however, evaluations against other important cattle ticks such as Amblyomma mixtum and R. annulatus, are needed. A transdisciplinary approach is required to incorporate different types of tools, such as genomics, transcriptomics and proteomics in order to better understand the pathogenicity/virulence mechanism in EPF against ticks. Laboratory tests have demonstrated the EPF efficacy to control susceptible and resistant/multiresistant tick populations; whereas, field tests have shown satisfactory control efficiency of M. anisopliae s.l. against different stages of R. microplus when applied both on pasture and on cattle. Epidemiological aspects of ticks and environmental factors are considered as components that influence the acaricidal behavior of the EPF. Finally, considering all these aspects, some recommendations are proposed for the use of EPF in integrated control schemes for livestock ticks.

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“…Extracellular peptidases play vital role in penetrating insect cuticle and contribute to pathogenicity of fungi (St. Leger, 1995;St. Leger et al, 1997;Yike, 2011;Semenova et al, 2020). Their vital roles in pathogenicity make them become markers of pathogenicity in fungi (Semenova et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Leger et al, 1997;Yike, 2011;Semenova et al, 2020). Their vital roles in pathogenicity make them become markers of pathogenicity in fungi (Semenova et al, 2020). In human fungal pathogens, secreted peptidase increased the fungal survival and virulence in Aspergillus fumigatus (Beauvais et al, 1997), Cryptococcus neoformans (Clarke et al, 2016) and Candida (Dutton et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Disruption of a C69-Family Cysteine Dipeptidase Gene Enhances Heat Shock and UV-B Tolerances in Metarhizium acridum

Guo

Cao

et al. 2020

Front. Microbiol.

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In fungi, peptidases play a crucial role in adaptability. At present, the roles of peptidases in ultraviolet (UV) and thermal tolerance are still unclear. In this study, a C69-family cysteine dipeptidase of the entomopathogenic fungus Metarhizium acridum, MaPepDA, was expressed in Escherichia coli. The purified enzyme had a molecular mass of 56-kDa, and displayed a high activity to dipeptide substrate with an optimal Ala-Gln hydrolytic activity at about pH 6.0 and 55 • C. It was demonstrated that MaPepDA is an intracellular dipeptidase localized in the cytosol, and that it is expressed during the whole fungal growth. Disruption of the MaPepDA gene increased conidial germination, growth rate, and significantly improved the tolerance to UV-B and heat stress in M. acridum. However, virulence and conidia production was largely unaffected in the MaPepDA mutant. Digital gene expression data revealed that the MaPepDA mutant had a higher UV-B and heat-shock tolerance compared to wild type by regulating transcription of sets of genes involved in cell surface component, cell growth, DNA repair, amino acid metabolism, sugar metabolism and some important signaling pathways of stimulation. Our results suggested that disruption of the MaPepDA could potentially improve the performance of fungal pesticides in the field application with no adverse effect on virulence and conidiation.

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Extracellular peptidases of insect-associated fungi and their possible use in biological control programs and as pathogenicity markers

Cited by 13 publications

References 67 publications

Insect pest management in the age of synthetic biology

Insect pest management in the age of synthetic biology

Entomopathogenic Fungi for Tick Control in Cattle Livestock From Mexico

Disruption of a C69-Family Cysteine Dipeptidase Gene Enhances Heat Shock and UV-B Tolerances in Metarhizium acridum

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