Extracellular Membranous Structures in a Stable L-form of Staphylococcus aureus

Eda, Tadahito; Kanda, Yuko; Mori, Claudio; Kimura, Sadao

doi:10.1099/00221287-103-1-189

Cited by 3 publications

(1 citation statement)

References 1 publication

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. aureus strain 209P, its protoplasts, and its stable L-form strain 209PL (6) were used in the present study. Cells of the parent strain were grown aerobically in brain heart infusion (BHI ; Difco Laboratories) at 37 C for 18 hr.…”

Section: Methodsmentioning

confidence: 99%

Morphological Detection of Filipin‐Sterol Complexes in the Cytoplasmic Membrane of Staphylococcal L‐Form

Nishiyama

Yamaguchi

1990

Microbiology and Immunology

View full text Add to dashboard Cite

Filipin, a sterol-specific antibiotic, and freeze-fracture electron microscopy were used to study the presence and distribution of sterol in the cytoplasmic membrane of stable staphylococcal L-form cells. Fixed cells were treated with filipin, and then observed by freeze-fracture electron microscopy.Freeze-fractured profiles of the L-form cells treated with filipin demonstrated irregular distribution of protuberances or pits of 25-30 nm, representing filipin-sterol complexes, on the protoplasmic fracture face (PF) and exoplasmic fracture face (EF) of the cytoplasmic membrane. In contrast, no such structure was detected in the filipin-treated parent cells or protoplasts.The results suggest that some sterol molecules, which are usually not found in staphylococcal or other bacterial cells, emerged on the cytoplasmic membrane after the cells were converted to the stable L-form.

show abstract

Section: Methodsmentioning

confidence: 99%