Conversion of Staphylococcus aureus Cells to Stable L‐Forms in a Liquid Growth Medium

Effect of Nalidixic Acid on the Conversion of Staphylococcus aureus Cells to L‐Forms in a Liquid Medium with 6‐Aminopenicillanic Acid and Lysozyme

“…1 show that the addition of nalidixic acid concomitantly with 6-APA and lysozyme to the liquid medium promoted conversion of S. aureus cells to L-forms. This effect of nalidixic acid was not observed when lysozyme was replaced with lysostaphin or N-acetylmuramidase from Streptomyces globisporus, which served as muralytic enzymes in the massive induction of L-forms in the liquid medium (11,12). These findings, together with the results indicating that nalidixic acid affects cell lysis induced by lysozyme (Fig.…”

“…and muralytic enzyme were added as L-form inducers (11,12). With the addition of 6-APA and lysozyme to the liquid medium, an exponentially growing culture of L-forms was obtained after a lag period (12 (13).…”