Extracellular matrix synthesis is required for the movement of sclerotome and neural crest cells on collagen

Sanders, Esmond J.; Prasad, S.; Cheung, E.

doi:10.1111/j.1432-0436.1988.tb00078.x

Cited by 13 publications

(4 citation statements)

References 39 publications

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“…However, pre-treatment of neural crest cells with p-D-xylosides or LACA, known to be respective inhibitors of the proteoglycan and interstitial collagen synthesislsecretion, did not affect attachment and locomotion on Col VI (not shown). This preliminar finding is consistent with previous observations indicating that neither proteoglycans nor secreted interstitial collagens are involved in the interaction of neural crest cells with various collagen types (Sanders et al, 1988;Perris et al, 1991a).…”

supporting

confidence: 93%

Collagen type VI in neural crest development: Distribution in situ and interaction with cells in vitro

Perris

Kuo

Glanville

et al. 1993

Developmental Dynamics

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We have examined the spatiotemporal distribution of collagen type VI (Col VI) during neural crest development in vivo and its ability to promote neural crest cell attachment and migration in vitro. An affinity purified antiserum and chain-specific monoclonal antibodies against chicken Col VI were employed to immunolocalize the collagen in tissue sections and by immunoblotting. At stages of initial neural crest cell migration, the al(V1) and 012(VI) chains were immunolocalized in apposition with basement membranes of the neural tube, somites, notochord and ectoderm, whereas no immunoreactivity was seen for the cw3(VI) chain. Immunoblotting analysis confirmed the expression of cwl(V1) and a2(VI) chains and the lack of detectable immunoreactivity for the cw3(VI) chain at these early phases of neural crest development. Conversely, at advanced phases of migration and following gangliogenesis, expression of cw3(VI) chain coincided with that of cul(V1) and a2(VI) chains in apposition with basement membranes, around the dorsal root ganglia, and in fibrillar arrangements within the developing dermis and ventral sclerotome. The ability of Col VI to promote neural crest cell attachment and migration was tested in vitro using quantitative assays for these processes. Both native microfilaments and isolated tetramers of Col VI strongly promoted neural crest cell attachment and migration. Optimal stimulation of neural crest cell adhesion and migration was dependent upon structural integrity of Col VI since unfolded and disassembled (Y chains only weakly promoted cell attachment and were virtually inactive in supporting cell movement. The importance of a native macromolecular organization of Col VI further was analyzed in experiments in which dissociated tetramers were reassociated by Ca2+-and temperature-dependent self-aggregation. In contrast to native microfilaments, these oligomeric complexes were less effective in promoting neural crest cell movement, but still retained the ability to stimulate maximal cell attachment. The results indicate that Col VI is a primary component of the extracellular matrix deposited along neural crest migratory pathways, where it mayparticipate in the regulation of cell movement by functioning as a migratory substrate. The ability of Col VI to promote neural crest cell adhesion and motility is highly dependent upon maintainance of a native macromolecular arrangement.0 1993 Wiley-Liss, Inc.

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supporting

confidence: 93%

Collagen type VI in neural crest development: Distribution in situ and interaction with cells in vitro

Perris

Kuo

Glanville

et al. 1993

Developmental Dynamics

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“…Fischman and obtained from the Developmental Studies Hybridoma Bank), was used to characterize muscle fibres and myogenic cells. Foci of chondrogenic nodules were identified by staining with 0.5% Alcian blue in 0.1 N HC1, pH 1.0, and subsequent removal of background staining with 3% acetic acid [24].…”

Section: Methodsmentioning

confidence: 99%

Effects of extracellular matrix components on the differentiation of chick embryo tail bud mesenchyme in culture

Griffith

Sanders

1991

Differentiation

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“…The interaction of neural crest cells with collagen did not appear to be mediated by homophilic interaction of cell surfaceassociated collagen with that in the substratum, since no collagen immunoreactivity could be detected on the surface of migrating neural crest cells in vivo and in vitro. In addition, other investigators have reported inhibitors of collagen synthesis do not significantly affect neural crest cell movement through Col I gels (Sanders et al 1988). Attachment of fibroblastic cells to denatured collagen is potentiated by exogenous fibronectin contained in serum or secreted by the cells (Grinnell and Minter, 1978;Chiquet et al 1979).…”

Section: Discussionmentioning

confidence: 96%

Collagens in avian neural crest development: distribution in vivo and migration-promoting ability in vitro

1991

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This study examines the spatiotemporal distribution of collagen (Col) types I-V and IX during neural crest development in vivo and their ability to support neural crest cell movement in vitro. Col I, III and IV were widespread throughout the embryo, including the neural crest migratory pathways, whereas Col II, V and IX preferentially localized to regions from which migrating neural crest cells were absent. Col I-IV and IX occurred both in association with basement membranes and within interstitial matrices, whereas Col V only was detected in juxtaposition to basement membranes. Although initially distributed throughout the rostrocaudal extent of the somitic sclerotome, Col I and III rearranged to the caudal portion with progressive neural crest cell migration through the rostral portion of the sclerotome. This rearrangement does not occur in neural crest-ablated embryos, suggesting that it is a direct consequence of neural crest cell migration. The perinotochordal matrix, avoided by neural crest cells, contained a metameric Col II/IX immunoreactivity along the rostrocaudal axis which alternated with that of Col I and III. In contrast, Col IV and V were not observed in this matrix, but lined the basement membranes of the notochord and ventrolateral neural tube. To determine their functional significance for neural crest cell migration in vivo, purified collagens were tested for their ability to promote neural crest cell motility in vitro. Neural crest cell migration on isolated collagens was most pronounced on Col I and IV, whereas Col II, V and the triple-helical fragment of Col VII were unable to support cell motility. Substrata created by copolymerization of Col I and fibronectin, or Col I and laminin-nidogen, supported cell motility better than Col I alone, whereas both Col V and a cartilage-type chondroitin sulfate proteoglycan reduced cell movement on Col I. Fibronectin bound to pre-immobilized monomeric Col I, II or V had a reduced ability to support neural crest cell movement when compared to fibronectin alone. A similar reduction was seen for Col IV bound to the low density heparan sulfate proteoglycan from the EHS mouse tumor. The results demonstrate that Col I-IX are differentially distributed in the early avian embryo. During neural crest development several of these collagens undergo dynamic reorganizations that correlate with the migration of neural crest cells. Furthermore, various collagens possess distinct abilities to support neural crest cell migration in vitro, and their migration-promoting activity can be modulated by their conformation and/or association with other matrix components.

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Extracellular matrix synthesis is required for the movement of sclerotome and neural crest cells on collagen

Cited by 13 publications

References 39 publications

Collagen type VI in neural crest development: Distribution in situ and interaction with cells in vitro

Collagen type VI in neural crest development: Distribution in situ and interaction with cells in vitro

Effects of extracellular matrix components on the differentiation of chick embryo tail bud mesenchyme in culture

Collagens in avian neural crest development: distribution in vivo and migration-promoting ability in vitro

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